MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following are advantageous to be used for expression of eukaryotic cells? |
| A. | Prokaryotic systems |
| B. | Yeast cells |
| C. | Fungi cells |
| D. | Algae cells |
| Answer» C. Fungi cells | |
| 2. |
Promoters such as GAL1 can be used to direct synthesis of either unmodified or fusion proteins. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 3. |
Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
PROMOTERS_SUCH_AS_GAL1_CAN_BE_USED_TO_DIRECT_SYNTHESIS_OF_EITHER_UNMODIFIED_OR_FUSION_PROTEINS._IS_THE_GIVEN_STATEMENT_TRUE_OR_FALSE??$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
Which of the following are advantageous to be used for expression of eukaryotic cells?$ |
| A. | Prokaryotic systems |
| B. | Yeast cells |
| C. | Fungi cells |
| D. | Algae cells |
| Answer» C. Fungi cells | |
| 6. |
How many deletion variations of GAL1 promoter are there? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 7. |
Transcription of GAL1 and GAL10 is suppressed in the presence of _______ |
| A. | glucose |
| B. | galactose |
| C. | maltose |
| D. | fructose |
| Answer» B. galactose | |
| 8. |
GAL1 is a controllable promoter and is obtained from genes for galactose metabolism. It is known for encoding ______ |
| A. | UDP-galactose-4-empirase |
| B. | Galactokinase |
| C. | Both UDP-galactose-4-empirase and galactokinase |
| D. | Glucokinase |
| Answer» C. Both UDP-galactose-4-empirase and galactokinase | |
| 9. |
Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 10. |
If YAC DNA is digested with BamHI and ECoRI, how many fragments are generated? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 11. |
Choose the incorrect statement for YAC vectors. |
| A. | The YAC molecule is approximately 10 kb in size |
| B. | It contains both yeast origin of replication and prokaryotic origin of replication |
| C. | It doesn’t contains ampicillin resistant gene |
| D. | It contains TEL sequence |
| Answer» D. It contains TEL sequence | |
| 12. |
TEL is the ______ sequence derived from the ends of ribosomal RNA-encoding molecules from the macromolecules of the protozoan Tetrahyena. |
| A. | centromeric |
| B. | telomeric |
| C. | can be either centromeric or telomeric |
| D. | can be present anywhere on the chromosome |
| Answer» C. can be either centromeric or telomeric | |
| 13. |
When YAC is used to clone DNA. What is the size of the DNA that can be cloned? |
| A. | Large (upto megabases) |
| B. | Small (upto few hundred bases) |
| C. | No size restriction |
| D. | Medium (upto kilobases) |
| Answer» B. Small (upto few hundred bases) | |