MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
When lacUV5 control system is used, addition of IPTG _______ the expression of _________ |
| A. | activates, T7 RNA polymerase |
| B. | inactivates, T7 RNA polymerase |
| C. | activates, T7 DNA polymerase |
| D. | inactivates, T7 DNA polymerase |
| Answer» B. inactivates, T7 RNA polymerase | |
| 2. |
Expression of T7 promoter- lac operator hybrid requires ___________ |
| A. | T7 RNA polymerase |
| B. | An inducer such as IPTG |
| C. | Both T7 RNA polymerase and inducer such as IPTG |
| D. | T7 DNA polymerase |
| Answer» D. T7 DNA polymerase | |
| 3. |
The tac promoter includes the lac operator and is regulated by a repressor. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
The tac promoter is made by _____ region of trp promoter and _____ region of the lacUV5 promoter. |
| A. | 10, 35 |
| B. | 35, 10 |
| C. | 10, 10 |
| D. | 35, 35 |
| Answer» C. 10, 10 | |
| 5. |
The promoter can be controlled by a repressor which is temperature sensitive. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
WHEN_LACUV5_CONTROL_SYSTEM_IS_USED,_ADDITION_OF_IPTG_________THE_EXPRESSION_OF__________?$ |
| A. | activates, T7 RNA polymerase |
| B. | inactivates, T7 RNA polymerase |
| C. | activates, T7 DNA polymerase |
| D. | inactivates, T7 DNA polymerase |
| Answer» B. inactivates, T7 RNA polymerase | |
| 7. |
Expression of T7 promoter- lac operator hybrid requires? |
| A. | T7 RNA polymerase |
| B. | An inducer such as IPTG |
| C. | Both T7 RNA polymerase and inducer such as IPTG |
| D. | T7 DNA polymerase |
| Answer» D. T7 DNA polymerase | |
| 8. |
The tac promoter includes lac operator and is regulated by repressor. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
The tac promoter is made by _____ region of trp promoter and _____ region of lacUV5 promoter. |
| A. | 10, 35 |
| B. | 35, 10 |
| C. | 10, 10 |
| D. | 35, 35 |
| Answer» C. 10, 10 | |
| 10. |
tac promoter is an example of which type of promoter? |
| A. | hybrid promoter |
| B. | fusion promoter |
| C. | lacZ promoter |
| D. | araBAD promoter |
| Answer» B. fusion promoter | |
| 11. |
The ______ operon encodes proteins involved in arabinose metabolism. |
| A. | araBCD |
| B. | araABD |
| C. | araBAD |
| D. | araDBA |
| Answer» D. araDBA | |
| 12. |
Choose the correct statement for rifampcin. |
| A. | It inactivates both E. coli polymerase and T7 polymerase |
| B. | It activates both E. coli polymerase and T7 polymerase |
| C. | It inhibits T7 polymerase but doesn’t inhibits E. coli polymerase |
| D. | It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase |
| Answer» E. | |
| 13. |
The promoter can be controlled by a repressor which is temperature sensitive. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
Lambda PL promoter is used in which vectors? |
| A. | Cloning vectors |
| B. | Expression vectors |
| C. | Both cloning and expression vectors |
| D. | Bacteriophage Mu |
| Answer» C. Both cloning and expression vectors | |
| 15. |
Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency. |
| A. | point mutations, decreases |
| B. | point mutations, increases |
| C. | frameshift mutations, increases |
| D. | frameshift mutations, increases |
| Answer» C. frameshift mutations, increases | |