MCQOPTIONS
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MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Protein-protein interactions such as in electron transport lead to activation of the reporter gene. |
| A. | True |
| B. | False |
| Answer» C. | |
| 2. |
_____ should enter the cell in the case of activation of the reporter gene for two hybrid system. |
| A. | Bait protein |
| B. | Prey protein |
| C. | Both bait and prey protein |
| D. | Either one of them |
| Answer» D. Either one of them | |
| 3. |
For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein. |
| A. | prey |
| B. | bait |
| C. | binding |
| D. | activation |
| Answer» C. binding | |
| 4. |
Sequences that can function as origins of replication are called as ___________ |
| A. | partial replicating sequences |
| B. | self replicating sequences |
| C. | autonomously replicating sequences |
| D. | modified replicating sequences |
| Answer» D. modified replicating sequences | |
| 5. |
In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
______SHOULD_ENTER_THE_CELL_IN_THE_CASE_OF_ACTIVATION_OF_REPORTER_GENE_FOR_TWO_HYBRID_SYSTEM.?$ |
| A. | Bait protein |
| B. | Prey protein |
| C. | Both bait and prey protein |
| D. | Either one of them |
| Answer» D. Either one of them | |
| 7. |
Protein-protein interactions such as in electron transport lead to activation of reporter gene. Is the given statement true or false?$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature? |
| A. | deactivated |
| B. | activated |
| C. | destroyed |
| D. | may be activated or not |
| Answer» E. | |
| 9. |
For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein? |
| A. | prey |
| B. | bait |
| C. | binding |
| D. | activation |
| Answer» C. binding | |
| 10. |
In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription. |
| A. | DNA binding, activation |
| B. | Activation, DNA binding |
| C. | Activation, transcription |
| D. | DNA binding, transcription |
| Answer» B. Activation, DNA binding | |
| 11. |
Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host? |
| A. | Bacterial host |
| B. | Fungal host |
| C. | Parasitic host |
| D. | Yeast host |
| Answer» E. | |
| 12. |
Sequences that can function as origins of replication are called as: |
| A. | partial replicating sequences |
| B. | self replicating sequences |
| C. | autonomously replicating sequences |
| D. | modified replicating sequences |
| Answer» D. modified replicating sequences | |
| 13. |
In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
Luciferase genes are also used at times for detection. Choose the correct statement for them. |
| A. | They are obtained from fire flies only |
| B. | The detection requires provision of substrate which produces light |
| C. | Enzymes such as beta-galactosidase requires substrate X-gluc to produce light |
| D. | Lucifearse genes are preferred over fluorescent proteins |
| Answer» C. Enzymes such as beta-galactosidase requires substrate X-gluc to produce light | |
| 15. |
GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP. |
| A. | It stands for Green Fluorescent Protein |
| B. | It is obtained from a bio-luminescent jellyfish and produces a protein aequorin which emits blue light |
| C. | The blue light is produced because of binding of sodium ions |
| D. | The absorbed blue light produces green light which can be detected further |
| Answer» D. The absorbed blue light produces green light which can be detected further | |
| 16. |
Promoter-probe vectors are used often. Choose the correct statement for these vectors. |
| A. | They are used for identifying sequences that can function as promoter in vivo |
| B. | It contains a reporter gene which contains its promoter along |
| C. | The reporter gene is having a cloning site |
| D. | After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening |
| Answer» B. It contains a reporter gene which contains its promoter along | |