Explore topic-wise MCQs in Genetic Engineering.

This section includes 17 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Cyanogen bromide is used for cleavage of junctions. It cleaves after ________ residues.

A. methionine
B. tryptophan
C. cysteine
D. phenolic acid
Answer» B. tryptophan
Discussion
2.

Enterokinase is an intestinal enzyme that converts _______ to ________

A. pepsinogen, pepsin
B. pepsin, pepsinogen
C. trypsinogen, trypsin
D. trypsin, trypsinogen
Answer» D. trypsin, trypsinogen
Discussion
3.

His tagged proteins can be eluted using EDTA or a pH gradient from the matrix.

A. True
B. False
Answer» B. False
Discussion
4.

Pel B protein is produced in plants and helps in the degradation of ______

A. vacuole
B. plasma membrane
C. cell wall
D. mitochondria
Answer» D. mitochondria
Discussion
5.

Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms.

A. True
B. False
Answer» B. False
Discussion
6.

A short peptide region fused to a protein of interest is known as ___________

A. tag
B. oligonucleotide
C. fragment
D. dimer
Answer» B. oligonucleotide
Discussion
7.

There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation.

A. solubility, phosphodiester bond formation
B. insolubility, phosphodiester bond formation
C. solubility, disulphide bond formation
D. insolubility, disulphidebond formation
Answer» D. insolubility, disulphidebond formation
Discussion
8.

CYANOGEN_BROMIDE_IS_USED_FOR_CLEAVAGE_OF_JUNCTIONS._IT_CLEAVES_AFTER__________RESIDUES.?$

A. methionine
B. tryptophan
C. cysteine
D. phenolic acid
Answer» B. tryptophan
Discussion
9.

Enterokinase is an intestinal enzyme that converts _______ to _______?

A. pepsinogen, pepsin
B. pepsin, pepsinogen
C. trypsinogen, trypsin
D. trypsin, trypsinogen
Answer» D. trypsin, trypsinogen
Discussion
10.

His tagged proteins can be eluted using EDTA or a pH gradient from the matrix. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
11.

Pel B protein is produced in plants and helps in degradation of ______

A. vacuole
B. plasma membrane
C. cell wall
D. mitochondria
Answer» D. mitochondria
Discussion
12.

Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

A. calcium ions
B. nickel ions
C. iron ions
D. fluorine ions
Answer» C. iron ions
Discussion
13.

Thioredexin protein contains two _______ residues.

A. cysteine
B. cystine
C. adenine
D. guanine
Answer» B. cystine
Discussion
14.

Maltose binding protein is the product of ______ gene in E.coli and located in ______

A. malE, nucleus
B. malD, nucleus
C. malE, periplasmic space
D. malD, periplasmic space
Answer» D. malD, periplasmic space
Discussion
15.

Glutathione-S-Transferase (GST) enzyme is used for conjunction of Glutathione molecules and is having a protective function in many organisms. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
16.

A short peptide region fused to a protein of interest is known as:

A. tag
B. oligonucleotide
C. fragment
D. dimer
Answer» B. oligonucleotide
Discussion
17.

There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding, ______ and ______ formation.

A. solubility, phosphodiester bond formation
B. insolubility, phosphodiester bond formation
C. solubility, disulphide bond formation
D. insolubility, disulphidebond formation
Answer» D. insolubility, disulphidebond formation
Discussion