Explore topic-wise MCQs in Genetic Engineering.

This section includes 21 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Which of the statement is incorrect for jumping PCR?

A. It is used in the case when the DNA fragment is degraded
B. In this type of PCR, the molecules are not long enough to span between the two primer sites
C. At the end of first round of synthesis, the extension of the molecule from the primer site to the end of the fragmented molecule takes place
D. It doesn’t leads to the formation of chimeric product
Answer» E.
Discussion
2.

Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?

A. The error caused because of polymerase is biased for the first position in the codon
B. The error caused because of polymerase is evenly distributed on all the positions in the codon
C. The error caused by sequence heterogeneity is mainly because of first position in the codon
D. The error caused by sequence heterogeneity is evenly distributed on all the codon positions
Answer» C. The error caused by sequence heterogeneity is mainly because of first position in the codon
Discussion
3.

Which of the statement is correct for misincorporation?

A. If direct sequencing has carried this misincorporation is a great problem
B. The erroneous molecules give strong signals than genuine molecules in case of misincorporation
C. If the misincorporation in cloned PCR products it is a problem
D. Even if the error is induced at an early stage it is not incorporated in many sequences
Answer» D. Even if the error is induced at an early stage it is not incorporated in many sequences
Discussion
4.

Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?

A. Amination of bases
B. Chemical cross-linking between the strands
C. Chemical cross-linking within the strands
D. Slowing down the polymerase
Answer» B. Chemical cross-linking between the strands
Discussion
5.

If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as ____________

A. Heterzygosity
B. Product heterogeneity
C. Population heterogeneity
D. Template heterogeneity
Answer» D. Template heterogeneity
Discussion
6.

During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

A. Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus
B. Direct sequencing can be used if the template DNA is heterozygous at the locus
C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
D. In the case several recombinants are used, it can’t go undetected
Answer» C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
Discussion
7.

There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination.

A. True
B. False
Answer» C.
Discussion
8.

How can the specificity of primer annealing be increased?

A. Use of short primers
B. Raising temperature
C. Adjusting the concentration of sodium ions
D. Using polymerase with proof reading activity
Answer» C. Adjusting the concentration of sodium ions
Discussion
9.

Which of the following conditions don’t contribute to wrong annealing to primer?

A. Chance complementarity
B. Conditions of annealing
C. The original sequence of the primers
D. Both the conditions of annealing and the original sequence don’t play any role
Answer» E.
Discussion
10.

WHICH_OF_THE_STATEMENT_IS_CORRECT_FOR_MISINCORPORATION??$

A. If direct sequencing is carried this misincorporation is a great problem
B. The erroneous molecules give strong signals than genuine molecules in case of misincorporation
C. If the misincorporation in cloned PCR products it is a problem
D. Even if the error is induced at an early stage it is not incorporated in many sequences
Answer» D. Even if the error is induced at an early stage it is not incorporated in many sequences
Discussion
11.

Which of the statement is incorrect for jumping PCR?$

A. It is used in the case when the DNA fragment is degraded
B. In this type of PCR, the molecules are not long enough to span between the two primer sites
C. At the end of first round of synthesis, the extension of the molecule from the primer site to the end of the fragmented molecule takes place
D. It doesn’t leads to the formation of chimeric product
Answer» E.
Discussion
12.

Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?$

A. The error caused because of polymerase is biased for the first position in the codon
B. The error caused because of polymerase is evenly distributed on all the positions in the codon
C. The error caused by sequence heterogeneity is mainly because of first position in the codon
D. The error caused by sequence heterogeneity is evenly distributed on all the codon positions
Answer» C. The error caused by sequence heterogeneity is mainly because of first position in the codon
Discussion
13.

If cytosine is deaminated, which of the base is formed?

A. Thymine
B. Guanine
C. Adenine
D. Uracil
Answer» E.
Discussion
14.

Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?#

A. Amination of bases
B. Chemical cross-linking between the strands
C. Chemical cross-linking within the strands
D. Slowing down the polymerase
Answer» B. Chemical cross-linking between the strands
Discussion
15.

If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as:

A. Heterzygosity
B. Product heterogeneity
C. Population heterogeneity
D. Template heterogeneity
Answer» D. Template heterogeneity
Discussion
16.

During amplification, there are chances of having a product of mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

A. Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus
B. Direct sequencing can be used if the template DNA is heterozygous at the locus
C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
D. In the case several recombinants are used, it can’t go undetected
Answer» C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
Discussion
17.

Which can be used as a precaution in order to minimize contamination?

A. Careful use and design of pipettes
B. Placing the pre-PCR and post-PCR stages in same rooms
C. Extracting the DNA along with surface layers
D. Use of primers carefully is not very important
Answer» B. Placing the pre-PCR and post-PCR stages in same rooms
Discussion
18.

There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination. Is the given statement true or false?

A. True
B. False
Answer» C.
Discussion
19.

How can specificity of primer annealing be increased?

A. Use of short primers
B. Raising temperature
C. Adjusting the concentration of sodium ions
D. Using polymerase with proof reading activity
Answer» C. Adjusting the concentration of sodium ions
Discussion
20.

Which of the following conditions don’t contribute to wrong annealing to primer?$

A. Chance complementarity
B. Conditions of annealing
C. The original sequence of the primers
D. Both the conditions of annealing and the original sequence don’t play any role
Answer» E.
Discussion
21.

Which of the statement holds for long-range PCR and in its relation?

A. It is the PCR in which longer templates are used
B. DNA polymerases which don’t have proof-reading activity give larger products
C. DNA polymerases’ processivity is not a measure to have larger products
D. It is PCR in which a mixture of enzymes is used to have larger products
Answer» E.
Discussion