MCQOPTIONS
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MCQOPTIONS
This section includes 71 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted. |
| A. | N terminal |
| B. | C terminal |
| C. | Both N & C terminal |
| D. | Middle |
| Answer» B. C terminal | |
| 52. |
Treatment of reticulocyte cells is done with EGTA. It chelates the calcium ions which are required for functioning of micrococcal nuclease. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 53. |
Lambda PL promoter is used in which vectors? |
| A. | Cloning vectors |
| B. | Expression vectors |
| C. | Both cloning and expression vectors |
| D. | Bacteriophage Mu |
| Answer» C. Both cloning and expression vectors | |
| 54. |
Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency. |
| A. | point mutations, decreases |
| B. | point mutations, increases |
| C. | frameshift mutations, increases |
| D. | frameshift mutations, increases |
| Answer» C. frameshift mutations, increases | |
| 55. |
A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins. |
| A. | coding, prokaryotes |
| B. | coding, eukaryotes |
| C. | non-coding, prokaryotes |
| D. | non-coding, eukaryotes |
| Answer» C. non-coding, prokaryotes | |
| 56. |
Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector. |
| A. | origin, replication |
| B. | end, replication |
| C. | end, stability |
| D. | origin, stability |
| Answer» E. | |
| 57. |
Mutation in gale gene or cpsA-E gene cluster is used for ___________ |
| A. | suppression of mucoidy |
| B. | activation of mucoidy |
| C. | suppression of UV sensitivity |
| D. | both suppression of mucoidy and UV sensitivity |
| Answer» B. activation of mucoidy | |
| 58. |
How many types of reporter gene constructs are there? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 59. |
The promoter can be controlled by a repressor which is temperature sensitive. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 60. |
For studying processes such as splicing and cleavage, RNA is required. Choose the correct statement for this. |
| A. | A mixture of different types of RNA is required |
| B. | Two types of RNA are required |
| C. | A few contaminating proteins are required |
| D. | Sodium hydroxide is required |
| Answer» D. Sodium hydroxide is required | |
| 61. |
Enterokinase is an intestinal enzyme that converts _______ to ________ |
| A. | pepsinogen, pepsin |
| B. | pepsin, pepsinogen |
| C. | trypsinogen, trypsin |
| D. | trypsin, trypsinogen |
| Answer» D. trypsin, trypsinogen | |
| 62. |
RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated. |
| A. | origin of replication, translation |
| B. | promoter, transcription |
| C. | promoter, translation |
| D. | origin of replication, transcription |
| Answer» C. promoter, translation | |
| 63. |
By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______ |
| A. | transcription, regardless of orientation |
| B. | transcription, only in one orientation |
| C. | translation, regardless of orientation |
| D. | translation, only in one orientation |
| Answer» B. transcription, only in one orientation | |
| 64. |
Capping can be introduced by the use of cap analogue. Which of the statement is true? |
| A. | Cap analogue can be introduced at the end of the transcript |
| B. | Cap analogue can be introduced at the start of the transcript |
| C. | Cap analogue can be introduced anywhere in the transcript |
| D. | Cap analogue can be introduced both at the end and starting of the transcript |
| Answer» C. Cap analogue can be introduced anywhere in the transcript | |
| 65. |
Little quantities of radiolabelled proteins are required for which of the following? |
| A. | co or post translational targeting |
| B. | modification of proteins |
| C. | both co or post translational targeting and modification of proteins |
| D. | crystallization for structural studies |
| Answer» D. crystallization for structural studies | |
| 66. |
__________ quantities of _______ protein are required for determination of properties in biochemical and biological assays. |
| A. | Small, non-radiolabelled |
| B. | Small, radiolabelled |
| C. | Large, radiolablelled |
| D. | Large, non-radiolabelled |
| Answer» E. | |
| 67. |
Pel B protein is produced in plants and helps in the degradation of ______ |
| A. | vacuole |
| B. | plasma membrane |
| C. | cell wall |
| D. | mitochondria |
| Answer» D. mitochondria | |
| 68. |
His tagged proteins can be eluted using EDTA or a pH gradient from the matrix. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't Say |
| Answer» B. False | |
| 69. |
Thioredexin protein contains two _______ residues. |
| A. | cysteine |
| B. | cystine |
| C. | adenine |
| D. | guanine |
| Answer» B. cystine | |
| 70. |
Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used. |
| A. | calcium ions |
| B. | nickel ions |
| C. | iron ions |
| D. | fluorine ions |
| Answer» C. iron ions | |
| 71. |
Maltose binding protein is the product of ______ gene in E.coli and located in ______ |
| A. | malE, nucleus |
| B. | malD, nucleus |
| C. | malE, periplasmic space |
| D. | malD, periplasmic space |
| Answer» D. malD, periplasmic space | |