MCQOPTIONS
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MCQOPTIONS
This section includes 71 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What is the function of T7 lysozyme? |
| A. | Attacking the peptidoglycan in bacterial cell walls |
| B. | Inhibitor of T7 polymerase |
| C. | Activator of T7 polymerase |
| D. | Both attacking the peptidogylcan in bacterial cell walls and inhibitor of T7 polymerase |
| Answer» E. | |
| 2. |
The enhancer trap system will _____ in the ______ |
| A. | not replicate independently, host |
| B. | replicate independently, host |
| C. | not replicate independently, vector |
| D. | replicate independently, vector |
| Answer» B. replicate independently, host | |
| 3. |
Lon protease is used for degradation of foreign proteins in E.coli cells. It is active against which type of proteins? |
| A. | Partially denatured proteins |
| B. | Completely denatured proteins |
| C. | Both partially and completely denatured proteins |
| D. | Intact proteins |
| Answer» D. Intact proteins | |
| 4. |
The reporter gene in enhancer trap system is preceded by ______ |
| A. | intron |
| B. | exon |
| C. | promoter |
| D. | origin of replication |
| Answer» B. exon | |
| 5. |
For co-expression of proteins, it is possible to have several independent sets of promoters and other signals in a single vector. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 6. |
Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 7. |
If transcription should not be carried out beyond the insert in the vector, then it should be linearized. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 8. |
If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium? |
| A. | Single stranded DNA |
| B. | Double stranded DNA |
| C. | Both single and double stranded DNA |
| D. | Circular DNA |
| Answer» D. Circular DNA | |
| 9. |
Choose the correct statement for rifampcin. |
| A. | It inactivates both E. coli polymerase and T7 polymerase |
| B. | It activates both E. coli polymerase and T7 polymerase |
| C. | It inhibits T7 polymerase but doesn’t inhibits E. coli polymerase |
| D. | It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase |
| Answer» E. | |
| 10. |
tac promoter is an example of which type of promoter? |
| A. | hybrid promoter |
| B. | fusion promoter |
| C. | lacZ promoter |
| D. | araBAD promoter |
| Answer» B. fusion promoter | |
| 11. |
The ______ operon encodes proteins involved in arabinose metabolism. |
| A. | araBCD |
| B. | araABD |
| C. | araBAD |
| D. | araDBA |
| Answer» D. araDBA | |
| 12. |
The tac promoter is made by _____ region of trp promoter and _____ region of the lacUV5 promoter. |
| A. | 10, 35 |
| B. | 35, 10 |
| C. | 10, 10 |
| D. | 35, 35 |
| Answer» C. 10, 10 | |
| 13. |
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site. |
| A. | ACG |
| B. | AUG |
| C. | UAC |
| D. | CAG |
| Answer» C. UAC | |
| 14. |
Lon mutation can lead to ______ and _______ |
| A. | mucoidy, UV sensitivity |
| B. | mucoidy, UV insensitivity |
| C. | overproduction of fats, UV sensitivity |
| D. | overproduction of fats, UV insensitivity |
| Answer» B. mucoidy, UV insensitivity | |
| 15. |
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 16. |
If it is desirable to express two or more proteins simultaneously, then it is known as ___________ |
| A. | hybrid of proteins |
| B. | fusion of proteins |
| C. | co-expression of proteins |
| D. | combination of proteins |
| Answer» D. combination of proteins | |
| 17. |
Cyanogen bromide is used for cleavage of junctions. It cleaves after ________ residues. |
| A. | methionine |
| B. | tryptophan |
| C. | cysteine |
| D. | phenolic acid |
| Answer» B. tryptophan | |
| 18. |
Ribosome binding site in E.coli is composed of which sequence primarily? |
| A. | -GAGG- |
| B. | -CTCC- |
| C. | -ATGG- |
| D. | -CGTT- |
| Answer» B. -CTCC- | |
| 19. |
The tac promoter includes the lac operator and is regulated by a repressor. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 20. |
Cloning vectors designed for the purpose of synthesis of RNA and proteins are known as ___________ |
| A. | Cloning vectors |
| B. | RNA vectors |
| C. | Bacteriophage vectors |
| D. | Expression vectors |
| Answer» E. | |
| 21. |
Which of the following is present in E. coli rrnB operon for rRNA? |
| A. | Initiation codon |
| B. | Termination codon |
| C. | Both initiation and termination codon |
| D. | Both are absent |
| Answer» D. Both are absent | |
| 22. |
Promoters are generally used after isolation from ___________ |
| A. | bacteriophage T7 |
| B. | bacteriophage SP6 |
| C. | baceriophage Mu |
| D. | both bacteriophage T7 and SP6 |
| Answer» E. | |
| 23. |
Sequences can be cloned flanking the reporter genes using the transposon tagging approach. The use of reporter genes to identify sequences expressed in this way is called as ___________ |
| A. | trapping |
| B. | tagging |
| C. | identification |
| D. | blotting |
| Answer» B. tagging | |
| 24. |
Isolation of RNA can be carried out easily from bacterial cells. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 25. |
Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________ |
| A. | hybrid protein |
| B. | fusion protein |
| C. | combination protein |
| D. | insert protein |
| Answer» C. combination protein | |
| 26. |
How many methods are there, which are used for protein synthesis in vitro? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 27. |
Guanylyl transferase and GTP are used for? |
| A. | Transcription |
| B. | Translation |
| C. | Capping of the message |
| D. | Packaging |
| Answer» D. Packaging | |
| 28. |
In fusion proteins, the reading frame in a vector is out of phase with the insert. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 29. |
Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______ |
| A. | hybrid intron, exon |
| B. | hybrid vector, exon |
| C. | hybrid intron, target DNA sequence |
| D. | hybrid vector, target DNA sequence |
| Answer» D. hybrid vector, target DNA sequence | |
| 30. |
Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 31. |
Expression of T7 promoter- lac operator hybrid requires ___________ |
| A. | T7 RNA polymerase |
| B. | An inducer such as IPTG |
| C. | Both T7 RNA polymerase and inducer such as IPTG |
| D. | T7 DNA polymerase |
| Answer» D. T7 DNA polymerase | |
| 32. |
A short peptide region fused to a protein of interest is known as ___________ |
| A. | tag |
| B. | oligonucleotide |
| C. | fragment |
| D. | dimer |
| Answer» B. oligonucleotide | |
| 33. |
When lacUV5 control system is used, addition of IPTG _______ the expression of _________ |
| A. | activates, T7 RNA polymerase |
| B. | inactivates, T7 RNA polymerase |
| C. | activates, T7 DNA polymerase |
| D. | inactivates, T7 DNA polymerase |
| Answer» B. inactivates, T7 RNA polymerase | |
| 34. |
There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation. |
| A. | solubility, phosphodiester bond formation |
| B. | insolubility, phosphodiester bond formation |
| C. | solubility, disulphide bond formation |
| D. | insolubility, disulphidebond formation |
| Answer» D. insolubility, disulphidebond formation | |
| 35. |
The hybrid intron is spliced from the transcript using the splice acceptor site in the vector. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 36. |
What is the use of adding micrococcal nuclease in the reticulocyte cells? |
| A. | It degrades DNA |
| B. | It degrades mRNA |
| C. | It degrades proteins |
| D. | It degrades RNA and untranslated DNA |
| Answer» C. It degrades proteins | |
| 37. |
As the activity of T7 polymerase is reduced by lysosome, what is an effect on the rate of synthesis after induction? |
| A. | Increase |
| B. | Decrease |
| C. | May increase or decrease |
| D. | No effect |
| Answer» C. May increase or decrease | |
| 38. |
Strong secondary structure in a message may affect ________ |
| A. | translation |
| B. | transcription |
| C. | both translation and transcription |
| D. | none of translation and transcription |
| Answer» B. transcription | |
| 39. |
If detection of enhancers is done it is called as ______ and for detection of transcribed sequences, it is called as ______ |
| A. | enhancer trapping, promoter trapping |
| B. | enhancer trapping, gene trapping |
| C. | promoter trapping, gene trapping |
| D. | promoter trapping, enhancer trapping |
| Answer» C. promoter trapping, gene trapping | |
| 40. |
In which of the following systems, transcription and translation are carried out together? |
| A. | Reticuloycte lysate |
| B. | Wheat gram extract |
| C. | Both in reticulocyte lysate and wheat gram extract |
| D. | S-30 extract |
| Answer» E. | |
| 41. |
If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid. |
| A. | few, translation |
| B. | few, transformation |
| C. | no, transformation |
| D. | no, translation |
| Answer» D. no, translation | |
| 42. |
In ________ transcription and translation is coupled. |
| A. | prokaryotes |
| B. | eukaryotes |
| C. | both prokaryotes and eukaryotes |
| D. | both prokaryotes and eukaryotes, but favourable in eukaryotes |
| Answer» B. eukaryotes | |
| 43. |
Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration can be known by measuring the activity. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 44. |
______ fluorescent protein should be used for determination of _______ of subcellular components. |
| A. | One, age |
| B. | One, location |
| C. | More than one, location |
| D. | More than one, age |
| Answer» D. More than one, age | |
| 45. |
Reticulocytes are immature red cells, which are obtained from ___________ |
| A. | rabbit |
| B. | pig |
| C. | human |
| D. | cow |
| Answer» B. pig | |
| 46. |
It is not possible to solubilise proteins from aggregated mass of proteins. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 47. |
For gene trap vector system, reporter gene and _____ are present. Reporter gene is _____ |
| A. | selectable marker, having promoter |
| B. | selectable marker, promoterless |
| C. | helper phage, promoterless |
| D. | helper phage, having promoter |
| Answer» C. helper phage, promoterless | |
| 48. |
It is also possible to generate a fusion protein in which ______ is encoded by the vector. |
| A. | N terminal |
| B. | C terminal |
| C. | Both N and C terminal |
| D. | Middle region |
| Answer» C. Both N and C terminal | |
| 49. |
Proteins at times are not soluble in the cell and form aggregates known as ___________ |
| A. | coagulation |
| B. | aggregated mass |
| C. | inclusion bodies |
| D. | insoluble mass |
| Answer» D. insoluble mass | |
| 50. |
For the production of unlabelled and huge amount of proteins, which of the following is true? |
| A. | Transcription is carried out in vivo and translation in vitro |
| B. | Transcription and translation both are carried out in vivo |
| C. | Transcription and translation both are carried out in vitro |
| D. | Transcription is carried out in vitro and translation in vivo |
| Answer» C. Transcription and translation both are carried out in vitro | |