Explore topic-wise MCQs in Genetic Engineering.

This section includes 19 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

The correct statement with respect to lacIq is __________

A. It is a mutant of lac repressor gene and decreases the level of repressor
B. It doesn’t control the lacZ gene
C. It is important for the vectors to replicate in a low copy number
D. It doesn’t titrate out the repressor produced by wild type Lac-I gene
Answer» B. It doesn’t control the lacZ gene
Discussion
2.

lacZΔM15 represents __________

A. fully present lacZ gene
B. it is not required in alpha complementation
C. it represents the lacZ gene with M15 mutation
D. it is partially deleted lacZ gene which is needed for alpha complementation
Answer» E.
Discussion
3.

endA mutation is often induced in host molecules. Choose the correct statement.

A. It activates the gene for DNA-specific endonuclease
B. It enhances the yield of plasmid DNA preparations only
C. It enhances the quality of plasmid DNA preparations only
D. It enhances both the quality and yield of plasmid DNA preparations
Answer» E.
Discussion
4.

Disablement is also done in plasmids. Choose the correct statement.

A. The strains carrying out the recombinant plasmids should not escape outside
B. Preferred strains are having mutations which allow their growth in wild
C. Mutations confer prototrophy
D. If the recombinants escape outside, there is no such harm
Answer» B. Preferred strains are having mutations which allow their growth in wild
Discussion
5.

Choose the incorrect statement with respect to dam and dcm genes?

A. Their enzymes are responsible for methylation of DNA
B. Dam protein methylates cytosine in –GATC-
C. Dcm methylates cytosine in-CCAGG- and –CCTGG- sequences
D. The extent of methylation also has effects on the efficiency with which the other restriction enzymes cleave the DNA in vitro
Answer» C. Dcm methylates cytosine in-CCAGG- and –CCTGG- sequences
Discussion
6.

Endogenous DNA-degrading system is not essential in determining transformation efficiency.

A. True
B. False
Answer» C.
Discussion
7.

The cell in which the recombinant molecules are propagated is termed as __________

A. host
B. vector
C. plasmid
D. carrier
Answer» B. vector
Discussion
8.

DISABLEMENT_IS_ALSO_DONE_IN_PLASMIDS._CHOOSE_THE_CORRECT_STATEMENT.?$

A. The strains carrying out the recombinant plasmids should not escape outside
B. Preferred strains are having mutations which allow their growth in wild
C. Mutations confer prototrophy
D. If the recombinants escape outside, there is no such harm
Answer» B. Preferred strains are having mutations which allow their growth in wild
Discussion
9.

lacZΔM15 represents:$#

A. fully present lacZ gene
B. it is not required in alpha complementation
C. it represents lacZ gene with M15 mutation
D. it is partially deleted lacZ gene which is needed for alpha complementation
Answer» E.
Discussion
10.

endA_mutation_is_often_induced_in_host_molecules._Choose_the_correct_statement.$

A. It activates the gene for DNA-specific endonuclease
B. It enhances the yield of plasmid DNA preparations only
C. It enhances the quality of plasmid DNA preparations only
D. It enhances both the quality and yield of plasmid DNA preparations
Answer» E.
Discussion
11.

The_correct_statement_with_respect_to_lacIq_is:

A. It is mutant of lac repressor gene and decreases the level of repressor
B. It doesn’t controls the lacZ gene
C. It is important for the vectors to replicate in a low copy number
D. It doesn’t titrates out the repressor produced by wild type lac-I gene
Answer» B. It doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t controls the lacZ gene
Discussion
12.

How many recombination systems are present in E. coli?

A. 1
B. 2
C. 3
D. 4
Answer» D. 4
Discussion
13.

Choose the incorrect statement in respect to dam and dcm genes?

A. Their enzymes are responsible for methylation of DNA
B. Dam protein methylates cytosine in –GATC-
C. Dcm methylates cytosine in-CCAGG- and –CCTGG- sequences
D. The extent of methylation also has effects on the efficiency with which the other restriction enzymes cleave the DNA in vitro
Answer» C. Dcm methylates cytosine in-CCAGG- and ‚Äö√Ñ√∂‚àö√ë‚àö¬®CCTGG- sequences
Discussion
14.

Methylation dependent restriction system (MDRS) are included in which class of restriction enzymes?

A. Type I
B. Type II
C. Type III
D. They are the product of mcrA, mcrB or mrr loci
Answer» E.
Discussion
15.

Which of the following is true for K strain for E. coli?

A. It contains of restriction-modification system which is encoded by hsDMRS locus
B. hsDR encodes for endonuclease which is responsible for cleaving the DNA sequence containing –AACGCNNTGC-
C. Cleavage is carried out when the second A is non methylated
D. hsDR mutation can be used for cleavage of incoming unprotected DNA
Answer» B. hsDR encodes for endonuclease which is responsible for cleaving the DNA sequence containing ‚Äö√Ñ√∂‚àö√ë‚àö¬®AACGCNNTGC-
Discussion
16.

Endogenous DNA-degrading system is not essential in determining transformation efficiency. The given statement is true or false?

A. True
B. False
Answer» C.
Discussion
17.

Some mutations are present which assist the uptake of DNA. Which of the statement is incorrect for it?

A. deoR is such a mutation which assists the uptake of DNA
B. It is a transcriptional regulator
C. It is having DNA binding activity
D. It controls expression of a set of genes involved ribonucleoside catabolism
Answer» E.
Discussion
18.

The cell in which the recombinant molecules are propagated is termed as:

A. host
B. vector
C. plasmid
D. carrier
Answer» B. vector
Discussion
19.

How will we choose which transformation method is to be used?

A. It depends on the available conditions for the reaction to take place
B. It depends on the efficiency which is required to be obtained
C. All the transformation methods can be used equivalently
D. Each reaction has been assigned a particular transformation method
Answer» C. All the transformation methods can be used equivalently
Discussion