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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The particle gun method consists of which of the following steps? |
| A. | The DNA of interest is absorbed onto microprojectile beads |
| B. | These beads are often made of aluminium |
| C. | The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads |
| D. | The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it |
| Answer» D. The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it | |
| 2. |
Transformation carried out using a particle gun is known as biolistic transformation. It falls under which category of transformation? |
| A. | Physical |
| B. | Chemical |
| C. | Electroporation |
| D. | Natural |
| Answer» B. Chemical | |
| 3. |
Electroporation is also used for taking up the DNA by the cells. It constitutes of ____________ |
| A. | inserting the DNA into the cells via an electric shock |
| B. | increased efficiency than both natural and chemical methods |
| C. | causing the least amount of damage in comparison to other methods |
| D. | decreased efficiency than both natural and chemical methods |
| Answer» B. increased efficiency than both natural and chemical methods | |
| 4. |
Viral infection can also be used to take up the DNA by the cells. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
Introduction of DNA molecules into the recipient organism is termed as ____________ |
| A. | transformation |
| B. | translation |
| C. | transduction |
| D. | transcription |
| Answer» B. translation | |
| 6. |
TRANSFORMATION_CARRIED_OUT_USING_A_PARTICLE_GUN_IS_KNOWN_AS_BIOLISTIC_TRANSFORMATION._IT_FALLS_UNDER_WHICH_CATEGORY_OF_TRANSFORMATION??$ |
| A. | Physical |
| B. | Chemical |
| C. | Electrporation |
| D. | Natural |
| Answer» B. Chemical | |
| 7. |
The_particle_gun_method_consists_of_which_of_the_following_steps?$ |
| A. | The DNA of interest is absorbed onto microprojectile beads |
| B. | These beads are often made of aluminium |
| C. | The explosion in a gun propels a macroprojectile forward which in turn propels microprojectile beads |
| D. | The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it |
| Answer» D. The macroprojectiles and microprojectiles both cross the perforated plate and hit the target tissue behind it | |
| 8. |
Electroporation is also used for taking up the DNA by the cells. It constitutes of? |
| A. | inserting the DNA into the cells via an electric shock |
| B. | increased efficiency than both natural and chemical methods |
| C. | causing the least amount of damage in comparison to other methods |
| D. | decreased efficiency than both natural and chemical methods |
| Answer» B. increased efficiency than both natural and chemical methods | |
| 9. |
Chemical transformation refers to the methods which use chemicals in order to carry out transformation. Which of the following statements is true with respect to it? |
| A. | Chemical transformation decreases the efficiency of transformation as compared to natural transformation |
| B. | Ice cold calcium solution followed by heat shock is responsible for affecting the efficiency of DNA uptake |
| C. | The mechanism responsible for it named as ‘the heat shock model’ |
| D. | Other complex mixtures such as those containing Manganese and hexamine cobalt can’t be used to affect the efficiency |
| Answer» C. The mechanism responsible for it named as ‚Äö√Ñ√∂‚àö√ë‚àö‚â§the heat shock model‚Äö√Ñ√∂‚àö√ë‚àö¬• | |
| 10. |
Viral infection can also be used to take up the DNA by the cells. The statement is true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 11. |
Plasmids can be classified into how many types depending on the genes present for their transformation? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 12. |
F plasmid is often used in conjugation. The correct statement is? |
| A. | The F plasmid encodes the factor which is transferred from one cell to another |
| B. | The factor encoded by the F plasmid is called as Filamentous (F) factor |
| C. | It is transferred from one cell to another by filament |
| D. | The bacteria must belong to same species to carry out the conjugation |
| Answer» B. The factor encoded by the F plasmid is called as Filamentous (F) factor | |
| 13. |
Which of the statements hold true for conjugation? |
| A. | Conjugation is the natural process of transferring DNA from one species to another |
| B. | It is the artificial process in case the cells are not able to take them up naturally |
| C. | The plasmids are transferred from one cell to another by physical contact |
| D. | The plasmids are transferred from one cell to another by chemical means |
| Answer» C. The plasmids are transferred from one cell to another by physical contact | |
| 14. |
Many bacterial species are having a natural ability to take up the exogenous DNA material. Which of the statement is not correct in regard to it? |
| A. | This ability is termed as competence |
| B. | It is not limited to particular growth phases |
| C. | The bacteria may develop new biochemical abilities under special conditions such as nutrient deprivation |
| D. | Induction of specific set of bacterial proteins may take place |
| Answer» C. The bacteria may develop new biochemical abilities under special conditions such as nutrient deprivation | |
| 15. |
Introduction of DNA molecules into the recipient organism is termed as: |
| A. | transformation |
| B. | translation |
| C. | transduction |
| D. | transcription |
| Answer» B. translation | |