Explore topic-wise MCQs in Genetic Engineering.

This section includes 21 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________

A. nascent peptide display
B. mRNA display
C. ribosome display
D. ribozyme display
Answer» D. ribozyme display
Discussion
2.

Choose the incorrect statement for panning.

A. It is a variant of ligand binding approach
B. The ligand is immobilized on the solid support and the cells are passed over it
C. If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand
D. They are not useful for scanning libraries in cultured mammalian cells
Answer» E.
Discussion
3.

The ligand should bind to a single protein only for the screening process.

A. True
B. False
Answer» B. False
Discussion
4.

In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody.

A. secondary, primary
B. primary, secondary
C. primary, tertiary
D. secondary, tertiary
Answer» C. primary, tertiary
Discussion
5.

In the case of ligand binding by the expressed protein, the library can be screened by ______________

A. immunochemically, by the use of antibodies
B. ligands if the sequence we are looking for encodes a protein specific to a ligand
C. using a specific DNA sequence which can bind to the protein encoded by the sequence of interest
D. all of the mentioned
Answer» E.
Discussion
6.

Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

A. DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes
B. The RNA is translated within oocytes
C. Screening is carried out on the basis of DNA
D. The screening is helpful for the proteins whose function can be easily screened such as transport of ions
Answer» D. The screening is helpful for the proteins whose function can be easily screened such as transport of ions
Discussion
7.

If the library to be screened and the host belongs to different species, then it is called as ___________

A. homologous selection
B. heterologous selection
C. intraspecies selection
D. mixed selection
Answer» C. intraspecies selection
Discussion
8.

If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

A. True
B. False
Answer» B. False
Discussion
9.

Choose the incorrect statement with respect to host used.

A. The host must carry mutation for the gene of interest
B. The host should not contain restriction enzymes
C. The host if having restriction enzymes, then the incoming DNA should not be methylated
D. The host should be deficient in recombination
Answer» D. The host should be deficient in recombination
Discussion
10.

IN_THE_CASE_OF_IMMUNOCHEMICAL_SCREENING,_THE_POSITION_OF__________ANTIBODY_IS_DETECTED_BY_________ANTIBODY.?$

A. secondary, primary
B. primary, secondary
C. primary, tertiary
D. secondary, tertiary
Answer» C. primary, tertiary
Discussion
11.

Choose the incorrect statement for panning.$

A. It is a variant of ligand binding approach.
B. The ligand is immobilized on the solid support and the cells are passed over it
C. If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand
D. They are not useful for scanning libraries in cultured mammalian cells
Answer» E.
Discussion
12.

The_ligand_should_bind_to_a_single_protein_only_for_the_screening_process._Is_the_given_statement_true_or_false?$

A. True
B. False
Answer» B. False
Discussion
13.

If_screening_is_carried_out_by_using_a_combination_of_nascent_peptide_and_mRNA,_then_it_is_called_as:

A. nascent peptide display
B. mRNA display
C. ribosome display
D. ribozyme display
Answer» D. ribozyme display
Discussion
14.

In the case of ligand binding by the expressed protein, the library can be screened by?

A. immunochemically, by the use of antibodies
B. ligands if the sequence we are looking for encodes a protein specific to a ligand
C. using a specific DNA sequence which can bind to the protein encoded by the sequence of interest
D. using all the above methods given above
Answer» E.
Discussion
15.

Xenopus oocyte cells are also used at time to carry out screening. Choose the incorrect statement for this procedure.

A. DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes
B. The RNA is translated within oocytes
C. Screening is carried out on the basis of DNA
D. The screening is helpful for the proteins whose function can be easily screened such as transport of ions
Answer» D. The screening is helpful for the proteins whose function can be easily screened such as transport of ions
Discussion
16.

The direct selection allows _______ number of recombinants to be screened and _______

A. less, slowly
B. less, quickly
C. more, quickly
D. more, slowly
Answer» D. more, slowly
Discussion
17.

If the library to be screened and the host belong to different species, then it is called as:

A. homologous selection
B. heterologous selection
C. intraspecies selection
D. mixed selection
Answer» C. intraspecies selection
Discussion
18.

If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
19.

Choose the incorrect statement in respect to host used.

A. The host must carry mutation for the gene of interest
B. The host should not contain restriction enzymes
C. The host if having restriction enzymes, then the incoming DNA should not be methylated
D. The host should be deficient in recombination
Answer» D. The host should be deficient in recombination
Discussion
20.

Choose the correct statement if the screening is carried out by screening by expression in vivo.

A. The proportion of recombinants carrying gene of interest is small
B. The recombinants carrying gene of interest don’t complement the host mutation
C. Mutation should be affecting many genes
D. Most of the products selected would be result of complementation
Answer» B. The recombinants carrying gene of interest don‚Äö√Ñ√∂‚àö√ë‚àö¬•t complement the host mutation
Discussion
21.

At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

A. 1
B. 2
C. 3
D. 4
Answer» C. 3
Discussion