MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 22 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Purification of DNA by using silica derivatized groups by DEAE is termed as ____________ |
| A. | ion exchange resin based method. |
| B. | silica based purification |
| C. | atom based resin exchange method |
| D. | packed bed purification |
| Answer» B. silica based purification | |
| 2. |
Which of the following components bind to the solid column made of silica, under high salt concentration? |
| A. | Proteins |
| B. | Polysaccharides |
| C. | Both proteins and polysaccharides |
| D. | Plasmid DNA |
| Answer» E. | |
| 3. |
Adsorption onto a solid phase support followed by elution is used as an alternative for separation of which component? |
| A. | chromosomal DNA |
| B. | plasmid DNA |
| C. | RNA alone |
| D. | other impurities |
| Answer» C. RNA alone | |
| 4. |
Treatment with exonuclease leads to removal of ____________ |
| A. | remnants of chromosomal DNA |
| B. | RNase |
| C. | plasmid DNA which is circularized |
| D. | proteins |
| Answer» B. RNase | |
| 5. |
Nucleic acid precipitated constitutes of ____________ |
| A. | plasmid DNA |
| B. | plasmid DNA, along with RNA and chromosomal DNA |
| C. | rna alone |
| D. | chromosomal DNA only |
| Answer» C. rna alone | |
| 6. |
The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol. |
| A. | False |
| B. | True |
| Answer» C. | |
| 7. |
Chromosomal or genomic DNA is separated by ____________ |
| A. | Sedimentation |
| B. | Dissolution in water |
| C. | Centrifugation |
| D. | Distillation |
| Answer» D. Distillation | |
| 8. |
Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it? |
| A. | Cell lysis |
| B. | Removal of proteins |
| C. | Removal of chromosomal DNA |
| D. | Dissolving plasmid in water |
| Answer» E. | |
| 9. |
ADSORPTION_ONTO_A_SOLID_PHASE_SUPPORT_FOLLOWED_BY_ELUTION_IS_USED_AS_AN_ALTERNATIVE_FOR_SEPARATION_OF_WHICH_COMPONENT:?$ |
| A. | chromosomal DNA |
| B. | plasmid DNA |
| C. | RNA alone |
| D. | other impurities |
| Answer» C. RNA alone | |
| 10. |
Purification of DNA by using silica derivatized groups by DEAE is termed as:$ |
| A. | ion exchange resin based method. |
| B. | silica based purification |
| C. | atom based resin exchange method |
| D. | packed bed purification |
| Answer» B. silica based purification | |
| 11. |
Which_of_the_following_components_bind_to_the_solid_column_made_of_silica,_under_high_salt_concentration?$ |
| A. | Proteins |
| B. | Polysaccharides |
| C. | Both proteins and polysaccharides |
| D. | Plasmid DNA |
| Answer» E. | |
| 12. |
The location of plasmid DNA can be visualized by addition of: |
| A. | bromophenol blue |
| B. | ethidium bromide |
| C. | ortho xylene |
| D. | texas red |
| Answer» C. ortho xylene | |
| 13. |
Which of the following components settles at the bottom? |
| A. | RNA |
| B. | Proteins |
| C. | Nicked DNA |
| D. | Supercoiled DNA |
| Answer» B. Proteins | |
| 14. |
Which of the following is correct with respect to caesium chloride centrifugation? |
| A. | Caesium is light in weight |
| B. | The dissolution of caesium and nucleic acids leads to the formation of gradients |
| C. | According to amount of supercoiling and A+T content, the DNA settles |
| D. | Nicked DNA settles below than supercoiled DNA |
| Answer» C. According to amount of supercoiling and A+T content, the DNA settles | |
| 15. |
Treatment with exonuclease leads to removal of? |
| A. | remnants of chromosomal DNA |
| B. | RNase |
| C. | plasmid DNA which is circularized |
| D. | proteins |
| Answer» B. RNase | |
| 16. |
Nucleic acid precipitated constitutes of: |
| A. | plasmid DNA |
| B. | plasmid DNA, along with RNA and chromosomal DNA |
| C. | RNA alone |
| D. | chromosomal DNA only |
| Answer» C. RNA alone | |
| 17. |
The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol. Is the statement true or false? |
| A. | False |
| B. | True |
| Answer» C. | |
| 18. |
Proteins can be removed via treatment by? |
| A. | Phenol and chloroform treatment |
| B. | Treatment with sodium hydroxide |
| C. | Chloroform treatment alone |
| D. | Centrifuging |
| Answer» B. Treatment with sodium hydroxide | |
| 19. |
Chromosomal or genomic DNA is separated by: |
| A. | Sedimentation |
| B. | Dissolution in water |
| C. | Centrifugation |
| D. | Distillation |
| Answer» D. Distillation | |
| 20. |
Cell lysis is carried out by which substance? |
| A. | Lysozyme and detergents |
| B. | Water |
| C. | Sugar solution |
| D. | Suphuric Acid |
| Answer» B. Water | |
| 21. |
How many methods are there for obtaining the plasmid DNA from the bacteria? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 22. |
Isolation of genomic DNA follows same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it? |
| A. | Cell lysis |
| B. | Removal of proteins |
| C. | Removal of chromosomal DNA |
| D. | Dissolving plasmid in water |
| Answer» E. | |