Explore topic-wise MCQs in Genetic Engineering.

This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?

A. They only add a single nucleotide
B. They only add a string of nucleotides and not a single nucleotide
C. Terminal transferase adds a series of nucleotides at the 3’ end
D. Taq polymerase adds a single nucleotide at the 5’ end of the PCR product
Answer» D. Taq polymerase adds a single nucleotide at the 5’ end of the PCR product
Discussion
2.

Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription?

A. RNA dependent DNA polymerase
B. DNA dependent RNA polymerase
C. RNA dependent RNA polymerase
D. DNA dependent DNA polymerase
Answer» C. RNA dependent RNA polymerase
Discussion
3.

Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases?

A. Because they are not having 3’-5’ proofreading exonucleolytic activity
B. Because they are not having 5’-3’ proofreading exonucleolytic activity
C. Because they are having slow rates of exonucleolytic activity
D. It is difficult to synthesize DNA from RNA
Answer» B. Because they are not having 5’-3’ proofreading exonucleolytic activity
Discussion
4.

Which of the following enzyme is said as reverse transcriptase?

A. DNA dependent DNA polymerase
B. RNA dependent RNA polymerase
C. RNA dependent DNA polymerase
D. DNA dependent RNA polymerase
Answer» D. DNA dependent RNA polymerase
Discussion
5.

Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________

A. polishing and end filling respectively
B. end filling and polishing respectively
C. polishing in both the cases
D. end filling in both the cases
Answer» B. end filling and polishing respectively
Discussion
6.

Polymerase can be defined as ______________

A. an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand
B. an enzyme used for removal of nucleotides from the DNA or RNA strand
C. an enzyme which can synthesize only a new DNA strand, not an RNA strand
D. an enzyme which can synthesize either a new DNA or an RNA strand but only when a strand is there
Answer» B. an enzyme used for removal of nucleotides from the DNA or RNA strand
Discussion
7.

WHICH_POLYMERASE_CAN_BE_USED_IN_CONJUNCTION_WITH_APPROPRIATE_PHAGE_PROMOTERS_IN_ORDER_TO_HAVE_HIGH_LEVELS_OF_SPECIFIC_TRANSCRIPTION??$

A. RNA dependent DNA polymerase
B. DNA dependent RNA polymerase
C. RNA dependent RNA polymerase
D. DNA dependent DNA polymerase
Answer» C. RNA dependent RNA polymerase
Discussion
8.

Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?$

A. They only add a single nucleotide
B. They only add a string of nucleotides and not a single nucleotide
C. Terminal transferase adds a series of nucleotides at the 3’ end
D. Taq polymerase adds a single nucleotide at the 5’ end of the PCR product
Answer» D. Taq polymerase adds a single nucleotide at the 5‚Äö√Ñ√∂‚àö√ë‚àö¬• end of the PCR product
Discussion
9.

Why reverse transciptase enzymes are having comparatively high error rates than other polymerases?

A. Because they are not having 3’-5’ proofreading exonucleolytic activity
B. Because they are not having 5’-3’ proofreading exonucleolytic activity
C. Because they are having slow rates of exonucleolytic activity
D. It is difficult to synthesize DNA from RNA
Answer» B. Because they are not having 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• proofreading exonucleolytic activity
Discussion
10.

Which of the following enzyme is said as reverse transciptase?

A. DNA dependent DNA polymerase
B. RNA dependent RNA polymerase
C. RNA dependent DNA polymerase
D. DNA dependent RNA polymerase
Answer» D. DNA dependent RNA polymerase
Discussion
11.

Thermostable DNA polymerases are very important in PCR. How are they obtained?

A. They are obtained by heating the bacteria manually over high temperatures
B. They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents
C. They are found everywhere in the nature
D. They are obtained by genetically modifying the E. coli bacteria with thermal stability property
Answer» C. They are found everywhere in the nature
Discussion
12.

Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as:

A. polishing and end filling respectively
B. end filling and polishing respectively
C. polishing in both the cases
D. end filling in both the cases
Answer» B. end filling and polishing respectively
Discussion
13.

The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?

A. The smaller fragment is having C terminal and the larger fragment is having N terminal
B. The smaller fragment is named as Klenlow fragment and the intact molecule is called as Kornberg fragment
C. The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity
D. Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity
Answer» D. Both the fragments are having 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• polymerase and 3‚Äö√Ñ√∂‚àö√ë‚àö¬•-5‚Äö√Ñ√∂‚àö√ë‚àö¬• exonuclease activity
Discussion
14.

Which of the following activity is not possible in the case of DNA polymerase I?

A. 3’-5’ exonuclease
B. 5’-3’ exonuclease
C. 5’-3’ DNA synthesis
D. 3’-5’ DNA synthesis
Answer» E.
Discussion
15.

Polymerase can be defined as:

A. an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand
B. an enzyme used for removal of nucleotides from the DNA or RNA strand
C. an enzyme which can synthesize only a new DNA strand, not a RNA strand
D. an enzyme which can synthesize either a new DNA or a RNA strand but only when a strand is there
Answer» B. an enzyme used for removal of nucleotides from the DNA or RNA strand
Discussion
16.

Basic classification of polymerases includes how many types?

A. 2
B. 3
C. 4
D. 5
Answer» E.
Discussion