Explore topic-wise MCQs in Genetic Engineering.

This section includes 22 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

If linkers are combined with other features such as a selectable marker, it is called as __________

A. cassette
B. modified linker
C. adaptors
D. induced linker
Answer» B. modified linker
Discussion
2.

There is no effect if the insert itself contains the restriction site.

A. True
B. False
Answer» C.
Discussion
3.

Linkers are often used in cloning. Choose the incorrect statement for linkers.

A. These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence
B. They are blunt ended molecules
C. They are ligated to staggered ended insert molecules by T4 DNA ligase
D. After treatment with enzyme, both the ends of the linker are staggered
Answer» D. After treatment with enzyme, both the ends of the linker are staggered
Discussion
4.

Choose the incorrect statement in respect to topoisomerase I.

A. It is used to cleave only one DNA strand
B. It leaves at the recognition sequence –C/TCCTT- and is covalently attached to one end of the cleaved molecule
C. The supercoiling of the DNA strand is altered and then it is sealed again by the enzyme
D. It is slower than the normal DNA ligase
Answer» E.
Discussion
5.

TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

A. It is based on the fact that a T residue is incorporated at the end of the PCR product
B. ‘A’ residue is incorporated into the end of the vector
C. It gives a low yield
D. It is preferred over blunt end ligation
Answer» E.
Discussion
6.

When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

A. forced cloning
B. orientation cloning
C. correct cloning
D. restricted cloning
Answer» B. orientation cloning
Discussion
7.

ccdB gene is used at times. Choose the correct statement with respect to this gene.

A. It is a control of cell birth gene
B. It is activated in complex vectors to increase the fraction of recombinants
C. If intramolecular ligation takes place this protein is released
D. It is often regarded as control of cell death and birth gene
Answer» C. If intramolecular ligation takes place this protein is released
Discussion
8.

There are various methods to distinguish whether a colony contains a recombinant or not. One such method is __________

A. blue white screening
B. checking whether replication is taking place or not
C. checking the number of copies
D. looking for the multiple cloning site
Answer» B. checking whether replication is taking place or not
Discussion
9.

TA_CLONING_IS_A_METHOD_USED_FOR_CLONING_OF_PCR_PRODUCTS._WHICH_OF_THE_STATEMENT_IS_CORRECT_WITH_RESPECT_TO_IT??$

A. It is based on the fact that a T residue is incorporated at the end of the PCR product
B. ‘A’ residue is incorporated into the end of the vector
C. It gives low yield
D. It is preferred over blunt end ligation
Answer» E.
Discussion
10.

Linkers are often used in cloning. Choose the incorrect statement for linkers.$

A. These are short chemically synthesized molecules that contain a particular restriction enzyme site within the sequence
B. They are blunt ended molecules
C. They are ligated to staggered ended insert molecules by T4 DNA ligase
D. After treatment with enzyme, both the ends of the linker are staggered
Answer» D. After treatment with enzyme, both the ends of the linker are staggered
Discussion
11.

Choose the incorrect statement in respect to topoisomerase I.$

A. It is used to cleave only one DNA strand
B. It cleaves at the recognition sequence –C/TCCTT- and is covalently attached to one end of the cleaved molecule
C. The supercoiling of the DNA strand is altered and then it is sealed again by the enzyme
D. It is slower than the normal DNA ligase
Answer» E.
Discussion
12.

If linkers are combined with other features such as a selectable marker, it is called as:

A. cassette
B. modified linker
C. adaptors
D. induced linker
Answer» B. modified linker
Discussion
13.

Choose the correct statement for adaptors.

A. They are blunt ended at both the ends
B. They are single stranded at both the ends
C. They may be single stranded at one end and other end may be blunt
D. They don’t have extra restriction sites within their sequence
Answer» D. They don‚Äö√Ñ√∂‚àö√ë‚àö¬•t have extra restriction sites within their sequence
Discussion
14.

There is no effect if the insert itself contains the restriction site. The given statement is true or false?

A. True
B. False
Answer» C.
Discussion
15.

What is the disadvantage of amplification of using PCR over natural cloning?

A. In PCR, there is no proof reading activity
B. In PCR, small fragments can’t be amplified
C. There is an A incorporated in PCR products, which makes cloning difficult
D. PCR takes more time as compared to natural cloning
Answer» B. In PCR, small fragments can‚Äö√Ñ√∂‚àö√ë‚àö¬•t be amplified
Discussion
16.

What happens if insert DNA is cut with two different restriction enzymes at the ends?

A. It is difficult to insert the fragment
B. The insert can be ligated in any orientation
C. The insert can be ligated in one orientation only
D. The amount of product increases
Answer» D. The amount of product increases
Discussion
17.

When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as:

A. forced cloning
B. orientation cloning
C. correct cloning
D. restricted cloning
Answer» B. orientation cloning
Discussion
18.

ccdB gene is used at times. Choose the correct statement in respect to this gene.

A. It is a control of cell birth gene
B. It is activated in complex vectors to increase the fraction of recombinants
C. If intramolecular ligation takes place this protein is released
D. It is often regarded as control of cell death and birth gene
Answer» C. If intramolecular ligation takes place this protein is released
Discussion
19.

What is the correct time for carrying out the alkaline phosphatase treatment?

A. After the cutting of vector has been done
B. Before the insert DNA and the vector are mixed
C. After the cutting of vector has been done but before the insert DNA and vector are mixed
D. After the mixing of insert DNA and vector
Answer» D. After the mixing of insert DNA and vector
Discussion
20.

Alkaline Phosphatase is used at times and the vector is treated with it. Choose the incorrect statement.

A. It removes 5’ terminal phosphate group from nucleic acids
B. The 5’ phosphate group is required for the ligation to take place
C. Two phosphate bonds should be formed for the complete ligation to take place
D. The ligation between vector and insert won’t take place
Answer» E.
Discussion
21.

At times it is observed that non-recombinant plasmids are more than that of recombinant plasmids. Choose the correct statement in regard to it.

A. It makes easier to get the recombinant plasmids from the mixture
B. The ratio of insert DNA to vector DNA is reduced to get recombinant plasmids
C. Alkaline phosphatase is used to get recombinant plasmids
D. Alkaline phosphatase method is less reliable in comparison to the ratio method
Answer» D. Alkaline phosphatase method is less reliable in comparison to the ratio method
Discussion
22.

There are various methods to distinguish whether a colony contains a recombinant or not. One of such method is:

A. blue white screening
B. checking whether replication is taking place or not
C. checking the number of copies
D. looking for the multiple cloning site
Answer» B. checking whether replication is taking place or not
Discussion