MCQOPTIONS
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MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Ligation enzymes are used for ligating newly synthesized okazaki fragments. What holds true for okazaki fragments? |
| A. | Okazaki fragments are short fragments of DNA formed on the leading strand |
| B. | Okazaki fragments are large fragments of DNA formed on the lagging strand |
| C. | Okazaki fragments are short fragments of DNA formed on the lagging strand |
| D. | Okazaki fragments are large fragments of DNA formed on the leading strand |
| Answer» D. Okazaki fragments are large fragments of DNA formed on the leading strand | |
| 2. |
Bacteriophage lambda is having a phage recombination system. Following are the characteristics of this system ____________ |
| A. | It is used for inserting phage genome into the bacterium |
| B. | It is used for inserting bacterial genome into the phage |
| C. | The specific site in bacteria is attB and that in phage is attP |
| D. | The specific sites in both of them are called as attP |
| Answer» B. It is used for inserting bacterial genome into the phage | |
| 3. |
Phage based recombination systems are used for ____________ |
| A. | cleaving the molecules at specific sites |
| B. | adding the molecules at specific sites |
| C. | breakage and rejoining the molecules at specific sites |
| D. | breakage at random sites |
| Answer» D. breakage at random sites | |
| 4. |
Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is ____________ |
| A. | Ligase |
| B. | Transciptase |
| C. | Transposase |
| D. | Endonuclease |
| Answer» D. Endonuclease | |
| 5. |
E. coli DNA ligase doesn’t require a cofactor. |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
Enzyme commonly used for carrying out ligation reaction is ____________ |
| A. | Transferase |
| B. | Reverse transciptase |
| C. | Ligase |
| D. | DNase |
| Answer» D. DNase | |
| 7. |
BACTERIOPHAGE_LAMBDA_IS_HAVING_A_PHAGE_RECOMBINATION_SYSTEM._FOLLOWING_ARE_THE_CHARACTERISTICS_OF_THIS_SYSTEM:?$ |
| A. | It is used for inserting phage genome into the bacterium |
| B. | It is used for inserting bacterial genome into the phage |
| C. | The specific site in bacteria is attB and that in phage is attP |
| D. | The specific sites in both of them are called as attP |
| Answer» B. It is used for inserting bacterial genome into the phage | |
| 8. |
Ligation_enzymes_are_used_for_ligating_newly_synthesized_okazaki_fragments._What_holds_true_for_okazaki_fragments?$ |
| A. | Okazaki fragments are short fragments of DNA formed on leading strand |
| B. | Okazaki fragments are large fragments of DNA formed on lagging strand |
| C. | Okazaki fragments are short fragments of DNA formed on lagging strand |
| D. | Okazaki fragments are large fragments of DNA formed on leading strand |
| Answer» D. Okazaki fragments are large fragments of DNA formed on leading strand | |
| 9. |
Phage based recombination systems are used for? |
| A. | cleaving the molecules at specific sites |
| B. | adding the molecules at specific sites |
| C. | breakage and rejoining the molecules at specific sites |
| D. | breakage at random sites |
| Answer» D. breakage at random sites | |
| 10. |
Mobile genetic elements can be transferred from one DNA portion to another. The enzyme carrying out this is: |
| A. | Ligase |
| B. | Transciptase |
| C. | Transposase |
| D. | Endonuclease |
| Answer» D. Endonuclease | |
| 11. |
Topoisomerase is also an enzyme which is used for carrying out ligation. The correct statement for topoisomerase is? |
| A. | They act only on double stranded molecules |
| B. | They alter the degree of supercoiling of DNA molecules |
| C. | They are less effective than conventional DNA ligase |
| D. | There are three types of topoisomerases |
| Answer» C. They are less effective than conventional DNA ligase | |
| 12. |
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism? |
| A. | AMP is added to the 5’ phosphate of one of the DNA molecule |
| B. | It leads to liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP |
| C. | The AMP is further displaced by an electrophilic attack |
| D. | The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule |
| Answer» B. It leads to liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP | |
| 13. |
E. coli DNA ligase doesn’t requires a cofactor. The statement is true or false?$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 14. |
If blunt ended ligations are to be carried out. Which of the following enzymes can be used? |
| A. | E. coli DNA ligase |
| B. | T4 DNA ligase |
| C. | Both of these enzymes act equally in carrying out blunt ended ligations |
| D. | None of them is able to carry out blunt ended ligations |
| Answer» C. Both of these enzymes act equally in carrying out blunt ended ligations | |
| 15. |
Which of the following statements is correct with respect to T4 DNA ligase? |
| A. | It can carry out only blunt ended ligations |
| B. | It doesn’t requires ATP |
| C. | It requires a phosphate group at 3’ end and a hydroxyl group at 5’ end for the molecule to be joined |
| D. | It is obtained from T4 bacteriophage upon infection by E. coli |
| Answer» E. | |
| 16. |
Enzyme commonly used for carrying out ligation reaction is: |
| A. | Transferase |
| B. | Reverse transciptase |
| C. | Ligase |
| D. | DNase |
| Answer» D. DNase | |