MCQOPTIONS
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MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
It is easier to subclone a restriction fragment if it belongs to? |
| A. | small gene |
| B. | large gene |
| C. | prokaryotic organism |
| D. | eukaryotic organism |
| Answer» C. prokaryotic organism | |
| 2. |
If mixed oligonucleotides are used, it is regarded as ______________ |
| A. | mixed mutagenesis |
| B. | multiple mutagenesis |
| C. | cassette mutagenesis |
| D. | polymutagenesis |
| Answer» D. polymutagenesis | |
| 3. |
Several different mutations can be induced at one site. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 5. |
Which of the following statement is incorrect for the synthesis of the second strand? |
| A. | The oligonucleotide is acting as a primer for the synthesis of the second strand |
| B. | DNA polymerase and dNTPs are added for synthesis |
| C. | The polymerase should have 5’-3’ exonuclease activity |
| D. | A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence |
| Answer» D. A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence | |
| 6. |
If the template is double stranded, they need to be separated before annealing of oligonucleotide. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
IF_MIXED_OLIGONUCLEOTIDES_ARE_USED,_IT_IS_REGARDED_AS:?$ |
| A. | mixed mutagenesis |
| B. | multiple mutagenesis |
| C. | cassette mutagenesis |
| D. | polymutagenesis |
| Answer» D. polymutagenesis | |
| 8. |
It_is_easier_to_subclone_a_restriction_fragment_if_it_belongs_to?$ |
| A. | small gene |
| B. | large gene |
| C. | prokaryotic organism |
| D. | eukaryotic organism |
| Answer» C. prokaryotic organism | |
| 9. |
Several different mutations can be induced at one site. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 10. |
How many sites can be mutated at a time? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | Many |
| Answer» E. | |
| 11. |
Once the double stranded molecule with mutation is introduced into E. coli for replication, how many types of molecules are produced? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 12. |
Which of the following statement is incorrect for synthesis of second strand? |
| A. | The oligonucleotide is acting as a primer for the synthesis of second strand |
| B. | DNA polymerase and dNTPs are added for synthesis |
| C. | The polymerase should have 5’-3’ exonuclease activity |
| D. | A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence |
| Answer» D. A polymerase having 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• exonuclease activity would degrade the primer that carries the mutant sequence | |
| 13. |
If the template is double stranded, they need to be separated before annealing of oligonucleotide. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA. |
| A. | complementary |
| B. | non-complementary |
| C. | can either be complementary or non-complementary |
| D. | not related |
| Answer» B. non-complementary | |
| 15. |
For mutagenesis without PCR, which of the following can be used as a template? |
| A. | Single stranded DNA |
| B. | Double stranded DNA |
| C. | Circular DNA |
| D. | Both single and double stranded DNA |
| Answer» E. | |
| 16. |
Bisulphite ions are used to deaminate ________ residues in _______ DNA. |
| A. | C, double stranded |
| B. | C, single stranded |
| C. | U, double stranded |
| D. | U, single stranded |
| Answer» C. U, double stranded | |