Explore topic-wise MCQs in Genetic Engineering.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

MANY_MAMMALIAN_CELLS_CONTAIN_THYMIDINE_KINASE,_THE_MAMMALIAN_ENZYME_USES_THE_ANALOGUE_______THAN_DOES_THE_VIRAL_ENZYME.?$

A. more efficiently
B. less efficiently
C. with same efficiency
D. either with same or more efficiency
Answer» C. with same efficiency
Discussion
2.

DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______$

A. polysaccharide, negatively
B. polysaccharide, positively
C. monosaccharide, positively
D. monosaccharide, negatively
Answer» C. monosaccharide, positively
Discussion
3.

A_wide_range_of_host_cell_lines_are_available_and_commonly_used_human_cell_lines_are_obtained_from:$

A. kidney
B. liver
C. lymphoblast from leukaemia patient
D. both kidney and lymphoblast from a leukaemia patient
Answer» E.
Discussion
4.

Splicing of the transcripts is necessary for efficient expression. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
5.

SV40 is a virus and it produces how many transcripts?

A. 1
B. 2
C. 3
D. 4
Answer» C. 3
Discussion
6.

In _____ cells, virus replication doesn’t take place and viral DNA _____$

A. non-permissive, is also not expressed
B. permissive, is also not expressed
C. non-permissive, can be expressed
D. non-permissive, is always expressed
Answer» D. non-permissive, is always expressed
Discussion
7.

Cells_whose_contents_have_been_removed_and_replaced,_by_swelling_and_shrinking_in_solutions_of_suitable_osmotic_strength_are_called_as:

A. protoplast
B. ghosts
C. shrunken cells
D. vacuole
Answer» C. shrunken cells
Discussion
8.

Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
9.

Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as selectable marker. This enzyme is involved in synthesis of _____ carbon units and is required for _____ biosynthesis.

A. two, nucleoside
B. two, nucleotide
C. one, nucleotide
D. one, nucleoside
Answer» E.
Discussion
10.

Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is:

A. DNA damaging agent
B. DNA synthesis promoter
C. Inhibiting RNA synthesis
D. Activating RNA synthesis
Answer» B. DNA synthesis promoter
Discussion
11.

Puromycin, is a protein synthesis inhibitor. It is conferred by_______ gene:

A. streptococcal
B. bacilovirus
C. streptomyces
D. both streptococcal and streptomyces
Answer» D. both streptococcal and streptomyces
Discussion
12.

Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for:

A. initiating protein synthesis
B. inhibiting protein synthesis
C. initiating DNA binding process
D. inhibiting DNA binding process
Answer» C. initiating DNA binding process
Discussion
13.

Cells deficient in thymidine kinase (TK) are also killed in HAT medium. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
14.

Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation.$

A. lithium acetate
B. calcium phosphate
C. sodium chloride
D. aluminum sulphate
Answer» C. sodium chloride
Discussion
15.

Lesch-Nyhan syndrome is caused by deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?

A. Hypoxanthine and thymidine
B. Thymidine
C. Aminopterin and thymidine
D. Hypoxanthine, thymidine and aminopterin (HAT medium)
Answer» E.
Discussion