MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Splicing of the transcripts is necessary for efficient expression. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 2. |
SV40 is a virus and it produces how many transcripts? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 3. |
In ___________ cells, virus replication doesn’t take place and viral DNA ___________ |
| A. | non-permissive, is also not expressed |
| B. | permissive, is also not expressed |
| C. | non-permissive, can be expressed |
| D. | non-permissive, is always expressed |
| Answer» D. non-permissive, is always expressed | |
| 4. |
Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________ |
| A. | protoplast |
| B. | ghosts |
| C. | shrunken cells |
| D. | vacuole |
| Answer» C. shrunken cells | |
| 5. |
DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______ |
| A. | polysaccharide, negatively |
| B. | polysaccharide, positively |
| C. | monosaccharide, positively |
| D. | monosaccharide, negatively |
| Answer» C. monosaccharide, positively | |
| 6. |
A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________ |
| A. | kidney |
| B. | liver |
| C. | lymphoblast from leukaemia patient |
| D. | both kidney and lymphoblast from a leukaemia patient |
| Answer» E. | |
| 7. |
Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme. |
| A. | more efficiently |
| B. | less efficiently |
| C. | with same efficiency |
| D. | either with same or more efficiency |
| Answer» C. with same efficiency | |
| 8. |
Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis. |
| A. | two, nucleoside |
| B. | two, nucleotide |
| C. | one, nucleotide |
| D. | one, nucleoside |
| Answer» E. | |
| 10. |
Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________ |
| A. | DNA damaging agent |
| B. | DNA synthesis promoter |
| C. | Inhibiting RNA synthesis |
| D. | Activating RNA synthesis |
| Answer» B. DNA synthesis promoter | |
| 11. |
Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene. |
| A. | streptococcal |
| B. | bacilovirus |
| C. | streptomyces |
| D. | both streptococcal and streptomyces |
| Answer» D. both streptococcal and streptomyces | |
| 12. |
Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________ |
| A. | initiating protein synthesis |
| B. | inhibiting protein synthesis |
| C. | initiating DNA binding process |
| D. | inhibiting DNA binding process |
| Answer» C. initiating DNA binding process | |
| 13. |
Cells deficient in thymidine kinase (TK) are also killed in HAT medium. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation. |
| A. | lithium acetate |
| B. | calcium phosphate |
| C. | sodium chloride |
| D. | aluminum sulphate |
| Answer» C. sodium chloride | |
| 15. |
Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following? |
| A. | Hypoxanthine and thymidine |
| B. | Thymidine |
| C. | Aminopterin and thymidine |
| D. | Hypoxanthine, thymidine and aminopterin (HAT medium) |
| Answer» E. | |