MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Self cleavage reaction can take place in? |
| A. | DNA |
| B. | RNA |
| C. | Both DNA and RNA |
| D. | Can take place in both but is preferred in DNA |
| Answer» C. Both DNA and RNA | |
| 2. |
Choose the correct statement for self cleavage reaction. |
| A. | It is nucleophilic attack reaction by 2’ hydroxyl of RNA |
| B. | It is nucleophilic attack reaction by 3’ hydroxyl of RNA |
| C. | It is nucleophilic attack reaction by 2’ hydroxyl of DNA |
| D. | It is nucleophilic attack reaction by 3’ hydroxyl of DNA |
| Answer» B. It is nucleophilic attack reaction by 3’ hydroxyl of RNA | |
| 3. |
Hepatitis delta virus capable of self cleavage. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
How much effect is there on the surrounding genes by the loxP sequence which is left after recombination has taken place? |
| A. | Little effect |
| B. | No effect |
| C. | Huge effect |
| D. | It depends on the nature of the surrounding gene |
| Answer» C. Huge effect | |
| 5. |
Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
CHOOSE_THE_CORRECT_STATEMENT_FOR_SELF_CLEAVAGE_REACTION.?$ |
| A. | It is nucleophilic attack reaction by 2’ hydroxyl of RNA |
| B. | It is nucleophilic attack reaction by 3’ hydroxyl of RNA |
| C. | It is nucleophilic attack reaction by 2’ hydroxyl of DNA |
| D. | It is nucleophilic attack reaction by 3’ hydroxyl of DNA |
| Answer» B. It is nucleophilic attack reaction by 3‚Äö√Ñ√∂‚àö√ë‚àö¬• hydroxyl of RNA | |
| 7. |
Self_cleavage_reaction_can_take_place_in?$ |
| A. | DNA |
| B. | RNA |
| C. | Both DNA and RNA |
| D. | Can take place in both but is preferred in DNA |
| Answer» C. Both DNA and RNA | |
| 8. |
Hepatitis delta virus capable of self cleavage. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
RNA molecule with catalytic activity is termed as________ |
| A. | ribosomes |
| B. | catalytic RNA |
| C. | reactive RNA |
| D. | ribozyme |
| Answer» E. | |
| 10. |
Excision of DNA flanked by loxP sequences is also known as _________ |
| A. | subtle excision |
| B. | croxing |
| C. | floxing |
| D. | sequence specific excision |
| Answer» D. sequence specific excision | |
| 11. |
The ability to control the expression of cre allows controlling what? |
| A. | recombination |
| B. | replication |
| C. | excision |
| D. | packaging |
| Answer» D. packaging | |
| 12. |
How much effect is there on the surrounding genes by the loxP sequence which is left after recombination has take place? |
| A. | Little effect |
| B. | No effect |
| C. | Huge effect |
| D. | It depends on the nature of the surrounding gene |
| Answer» C. Huge effect | |
| 13. |
Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
Choose the incorrect statement for cre-lox excision. |
| A. | The chromosomal copy of the target gene replaces the target gene flanked by loxP sites |
| B. | The second step is supply of Cre recombinase |
| C. | Integration of cre takes place under a controllable promoter followed by induction of the promoter |
| D. | Induction results in expression of cre, recombination along loxP sites and excision of the sequence between |
| Answer» B. The second step is supply of Cre recombinase | |
| 15. |
The ________ protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP. |
| A. | cre recombinase |
| B. | gene II |
| C. | gene IV |
| D. | gene VIII |
| Answer» B. gene II | |