MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
After carrying out the cloning experiment, the cells are plated on agar. Along with agar, it also contains antibiotic resistant genes, X-gal and an inducer of lacZ gene. Which of the following would grow? |
| A. | Cells that have taken up plasmid DNA |
| B. | Cells that have taken up genomic DNA |
| C. | Cells having no insert |
| D. | Cells either having no insert or having genomic DNA |
| Answer» B. Cells that have taken up genomic DNA | |
| 2. |
Transformation efficiency is defined as __________ |
| A. | ratio of transformed colonies by microgram of sample DNA that is to be inserted |
| B. | ratio of transformed colonies by amount of sample DNA that is to be inserted |
| C. | ratio of transformed colonies by microgram of plasmid DNA |
| D. | ratio of transformed colonies by amount of plasmid DNA |
| Answer» E. | |
| 3. |
Insertion of DNA into lacZ gene may lead to disruption of the gene function. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Molecules having new combination of sequences that were not present before are called as __________ |
| A. | intermolecular ligants |
| B. | recombinants |
| C. | couple |
| D. | intramolecular ligants |
| Answer» C. couple | |
| 5. |
AFTER_CARRYING_OUT_THE_CLONING_EXPERIMENT,_THE_CELLS_ARE_PLATED_ON_AGAR._ALONG_WITH_AGAR,_IT_ALSO_CONTAINS_ANTIBIOTIC_RESISTANT_GENES,_X-GAL_AND_AN_INDUCER_OF_LACZ_GENE._WHICH_OF_THE_FOLLOWING_WOULD_GROW??$ |
| A. | Cells that have taken up plasmid DNA |
| B. | Cells that have taken up genomic DNA |
| C. | Cells having no insert |
| D. | Cells either having no insert or having genomic DNA |
| Answer» B. Cells that have taken up genomic DNA | |
| 6. |
Transformation efficiency is defined as? |
| A. | ratio of transformed colonies by microgram of sample DNA that is to be inserted |
| B. | ratio of transformed colonies by amount of sample DNA that is to be inserted |
| C. | ratio of transformed colonies by microgram of plasmid DNA |
| D. | ratio of transformed colonies by amount of plasmid DNA |
| Answer» E. | |
| 7. |
Insertion of DNA into lacZ gene may lead to disruption of the gene function. This given statement is true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Molecules having new combination of sequences that were not present before are called as: |
| A. | intermolecular ligants |
| B. | recombinants |
| C. | couple |
| D. | intramolecular ligants |
| Answer» C. couple | |
| 9. |
Which of the following orientation is not propagated to later stages? |
| A. | Circular plasmids having genomic DNA |
| B. | Intramolecular ligation of plasmids |
| C. | Linear molecules |
| D. | Head to head ligation of two DNA plasmids |
| Answer» D. Head to head ligation of two DNA plasmids | |
| 10. |
Which of the characteristics is present in lacZ gene? |
| A. | It encodes for beta galactosidase enzyme |
| B. | Beta galactosidase enzyme is responsible for cleaving monosaccharides into the constituent elements |
| C. | It doesn’t cleaves a substrate called as X-gal |
| D. | But if X-gal is cleaved, it liberates pink coloured dye |
| Answer» B. Beta galactosidase enzyme is responsible for cleaving monosaccharides into the constituent elements | |
| 11. |
bla is a gene, which is incorrect for it? |
| A. | It is an antibiotic resistance genes |
| B. | Antibiotic acts by blocking the cross-linking of the bacterial cell wall and thus leading to lysing of cells |
| C. | It encodes beta lactamase enzyme |
| D. | The beta lactam ring is activated |
| Answer» E. | |
| 12. |
Which of the following characteristic is not present in a plasmid on a general basis? |
| A. | Multiple cloning site (MCS) |
| B. | Origin of replication (ori) |
| C. | Antibiotic resistance gene |
| D. | Beta galactose genes |
| Answer» E. | |
| 13. |
Plasmids are used for carrying out the cloning procedure. Which of the statement is true for plasmids? |
| A. | Bacterial plasmids are linear in nature |
| B. | They are single stranded |
| C. | Insertion of DNA into plasmid allows it to be propagated in host cells and they are known as vectors because of their this property |
| D. | They are not capable of replication in bacteria |
| Answer» D. They are not capable of replication in bacteria | |