MCQOPTIONS
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MCQOPTIONS
This section includes 34 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is/are commonly used promoter of ? |
| A. | Galactokinase (GAL 1) |
| B. | Alcohol dehydrogenase II (ADH 2) |
| C. | Phosphoglycerate kinase (PGK) |
| D. | All of the above |
| Answer» E. | |
| 2. |
Yeast Replicating plasmids (YRp) carry |
| A. | centromere |
| B. | telomere |
| C. | autonomously replicating sequence |
| D. | both (a) and (c) |
| Answer» D. both (a) and (c) | |
| 3. |
Which of the following viruses are used as vectors for transferring genes to animal cells? |
| A. | SV 40 virus |
| B. | Bovine papilloma virus (BVP) |
| C. | Retroviruses |
| D. | All of these |
| Answer» E. | |
| 4. |
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are |
| A. | transcriptional regulators and are essential for viral replication |
| B. | not necessary for viral replication |
| C. | coding for virus coat protein |
| D. | important for packaging of viral DNA into viral head |
| Answer» B. not necessary for viral replication | |
| 5. |
Retroviruses have advantage for being used as vector for animal cells because |
| A. | they cover a wide host range |
| B. | infection does not lead to cell death, infected cells produce virus over an indefinite period |
| C. | viral gene expression is driven by strong promoters |
| D. | all of the above |
| Answer» E. | |
| 6. |
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA |
| A. | episomally only |
| B. | integrated only |
| C. | either episomally or integrated at high copy number |
| D. | either episomally or integrated at low copy number |
| Answer» D. either episomally or integrated at low copy number | |
| 7. |
Cloning genes in the yeast offer which of following advantage(s)? |
| A. | Over production of proteins of commercial value |
| B. | Ability to glycosylate proteins during secretion |
| C. | Ability to clone large piece of DNA |
| D. | All of the above |
| Answer» E. | |
| 8. |
SV 40 vectors, in which late region replacements are done, require |
| A. | COS cell line |
| B. | SV 40 helper virus |
| C. | pBR 322 vector |
| D. | none of these |
| Answer» C. pBR 322 vector | |
| 9. |
Which of the following are produced in high copy number? |
| A. | YEp |
| B. | YRp |
| C. | Both (a) and (b) |
| D. | YCp |
| Answer» D. YCp | |
| 10. |
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is |
| A. | His 3 |
| B. | Lys 2 |
| C. | Ura 3 |
| D. | Trp 1 |
| Answer» D. Trp 1 | |
| 11. |
Which of the following can be used for reducing the size of gene inserts containing introns? |
| A. | Vaccinia virus |
| B. | Baculo virus |
| C. | Retro virus |
| D. | none of these |
| Answer» D. none of these | |
| 12. |
Bovine papilloma virus has a circular double stranded DNA genome of about |
| A. | 2.5 kb |
| B. | 5.2 kb |
| C. | 7.9 kb |
| D. | 9.5 kb |
| Answer» D. 9.5 kb | |
| 13. |
Retroviruses consist of |
| A. | Ds DNA genome |
| B. | Ss DNA genome |
| C. | RNA genome |
| D. | cDNA genome |
| Answer» D. cDNA genome | |
| 14. |
Which of the following(s) is/are the most commonly used markers in yeast? |
| A. | His 3 |
| B. | Ura 3 |
| C. | Trp 1 |
| D. | All of these |
| Answer» E. | |
| 15. |
Maximum insert size in case of adenovirus vectors is |
| A. | 6-8 kb |
| B. | 4-6 kb |
| C. | 10-12 kb |
| D. | 20 kb |
| Answer» B. 4-6 kb | |
| 16. |
Lytic infection of SV 40 does not occur in |
| A. | permissive cells |
| B. | non permissive cells |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» C. both (a) and (b) | |
| 17. |
Moloney-Mu-leukemia virus is an example of |
| A. | Vaccinia virus |
| B. | Baculo virus |
| C. | Retro virus |
| D. | none of these |
| Answer» D. none of these | |
| 18. |
The size of the genome of SV 40 is |
| A. | 7.2 kb |
| B. | 69 kb |
| C. | 5.2 kb |
| D. | 110 kb |
| Answer» D. 110 kb | |
| 19. |
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors? |
| A. | Allows transformation by using crossing over |
| B. | Has a origin of replication and rep genes |
| C. | Carries autonomously replicating sequence |
| D. | Carries centromere and telomere sequences |
| Answer» C. Carries autonomously replicating sequence | |
| 20. |
Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of |
| A. | Vaccinia virus |
| B. | Baculo virus |
| C. | Papilloma virus |
| D. | Retrovirus |
| Answer» C. Papilloma virus | |
| 21. |
Polyhedrin protein is |
| A. | non essential for baculo virus replication |
| B. | major component of protein matrix in which viruses are embedded |
| C. | both (a) and (b) |
| D. | essential for baculo virus replication |
| Answer» D. essential for baculo virus replication | |
| 22. |
Maximum amount of foreign DNA introduced into SV 40 viruses is |
| A. | 10 kb |
| B. | 5 kb |
| C. | 2.5 kb |
| D. | 1 kb |
| Answer» D. 1 kb | |
| 23. |
Recombinant SV40 viruses introduce the foreign DNA into cells |
| A. | with the DNA mediated transfection |
| B. | without the DNA mediated transfection |
| C. | with the RNA mediated transfection |
| D. | without the RNA mediated transfection |
| Answer» C. with the RNA mediated transfection | |
| 24. |
Which one of the following is the most stable vector? |
| A. | YEp |
| B. | YRp |
| C. | YAC |
| D. | YIp |
| Answer» D. YIp | |
| 25. |
SV 40 vectors, in which early region replacements are carried out, require |
| A. | COS cell line |
| B. | SV 40 helper virus |
| C. | pBR 322 vector |
| D. | none of these |
| Answer» B. SV 40 helper virus | |
| 26. |
Autonomously replicating sequence is not present in |
| A. | YIp |
| B. | YRp |
| C. | YCp |
| D. | YAC |
| Answer» B. YRp | |
| 27. |
In case of Baculoviruses, the foreign gene |
| A. | is inserted downstream of polyhedrin promoter |
| B. | is inserted downstream of viral origin of replication |
| C. | is inserted between the polyhedrin promoter and downstream sequences |
| D. | is inserted randomly in viral genome |
| Answer» D. is inserted randomly in viral genome | |
| 28. |
Cos cell line is |
| A. | cohesive end site of phage |
| B. | derivative of permissive CV-1 monkey cell line |
| C. | cohesive initial site of phage |
| D. | derivative of non-permissive CV-1 monkey cell line |
| Answer» C. cohesive initial site of phage | |
| 29. |
Which of the following histone is not present in SV40 virus? |
| A. | H1 |
| B. | H2A |
| C. | H3 |
| D. | H4 |
| Answer» B. H2A | |
| 30. |
YAC vectors can be used to obtain artificial chromosomes because it carries |
| A. | centromere |
| B. | telomere |
| C. | autonomously replicating sequence |
| D. | all of these |
| Answer» E. | |
| 31. |
Which of the following promoters is repressed in presence of glucose? |
| A. | Galactokinase (GAL 1) |
| B. | Acid phosphatase (PHOS) |
| C. | Both (a) and (b) |
| D. | Phosphoglycerate kinase (PGK) |
| Answer» B. Acid phosphatase (PHOS) | |
| 32. |
-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is |
| A. | His 3 |
| B. | Lys 2 |
| C. | Ura 3 |
| D. | Trp 1 |
| Answer» C. Ura 3 | |
| 33. |
Which of the following is/are commonly used promoter of S. cerevisiae? |
| A. | Galactokinase (GAL 1) |
| B. | Alcohol dehydrogenase II (ADH 2) |
| C. | Phosphoglycerate kinase (PGK) |
| D. | All of the above |
| Answer» E. | |
| 34. |
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors? |
| A. | Contains centromeric sequences |
| B. | Segregates during meiosis and mitosis |
| C. | Functions as true chromosome |
| D. | All of the above |
| Answer» E. | |