MCQOPTIONS
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MCQOPTIONS
This section includes 45 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following event occurs during third phase of growth of ? |
| A. | Concentration of antibiotic increases in the medium |
| B. | Autolysis of the medium starts |
| C. | Slight rise in pH due to liberation of ammonia |
| D. | All of the above |
| Answer» E. | |
| 2. |
During which phase of growth of maximum antibiotic production takes place |
| A. | during the first phase |
| B. | during the second phase |
| C. | during the third phase |
| D. | same in all the phases |
| Answer» C. during the third phase | |
| 3. |
Which of the following event occurs during second phase of growth of ? |
| A. | Synthesis of penicillin is high |
| B. | Mycelial mass increases |
| C. | pH increase |
| D. | Both (a) and (b) |
| Answer» E. | |
| 4. |
Which of the following changes occur during first phase of growth of ? |
| A. | Growth of mycelia occurs |
| B. | Ammonia is liberated in the medium |
| C. | Lactic acid present in the corn steep liquor is utilized at the maximum rate |
| D. | All of the above |
| Answer» E. | |
| 5. |
Antibiotics are typically produced in fed batch reactors because |
| A. | antibiotic yields are generally higher when cells enter the stationary phase |
| B. | the precursors are often toxic to the cells |
| C. | antibiotic yields are generally higher when cell growth slows |
| D. | All of the above |
| Answer» E. | |
| 6. |
Which of the following precursor is added in the medium to get penicillin G? |
| A. | Phenyl carbamic acid |
| B. | Phenyl acetic acid |
| C. | Ammonium sulphate |
| D. | Ammonium chloride |
| Answer» C. Ammonium sulphate | |
| 7. |
At normal pH, penicillin remains in |
| A. | aqueous phase |
| B. | solvent phase |
| C. | both (a) and (b) |
| D. | precipitates |
| Answer» B. solvent phase | |
| 8. |
Penicillin is produced by |
| A. | aerobic fermentation |
| B. | anaerobic fermentation |
| C. | aerobic fermentation followed by anaerobic fermentation |
| D. | anaerobic fermentation followed by aerobic fermentation |
| Answer» B. anaerobic fermentation | |
| 9. |
Which of the following changes occur during first phase of growth of Penicillium chrysogenum? |
| A. | Growth of mycelia occurs |
| B. | Ammonia is liberated in the medium |
| C. | Lactic acid present in the corn steep liquor is utilized at the maximum rate |
| D. | All of the above |
| Answer» E. | |
| 10. |
Media composition for the production of streptomycin is |
| A. | soybean meal, glucose, peptone, malt extract, sodium chloride |
| B. | soybean meal, glucose, peptone, malt extract, ammonium chloride |
| C. | soybean meal, glucose, peptone, malt extract, calcium carbonate |
| D. | soybean meal, glucose, peptone, malt extract, ammonium sulphate |
| Answer» B. soybean meal, glucose, peptone, malt extract, ammonium chloride | |
| 11. |
In monoclonal antibody technology, tumor cells that can replicate endlessly are fused with mammalian cells that produce an antibody. The result of this cell fusion is a |
| A. | hybridoma |
| B. | myeloma |
| C. | natural killer cell |
| D. | lymphoblast |
| Answer» B. myeloma | |
| 12. |
Some cross reactions with monoclonal antibodies (MAbs) can occur. Unexpected cross reactions occur more frequently with |
| A. | Ig MAbs |
| B. | IgG |
| C. | IgA |
| D. | IgE |
| Answer» B. IgG | |
| 13. |
The cross linkage of antigens by antibodies is known as |
| A. | agglutination |
| B. | complement fixation |
| C. | a cross reaction |
| D. | all of these |
| Answer» B. complement fixation | |
| 14. |
Which of the following event occurs during second phase of growth of P. chrysogenum? |
| A. | Synthesis of penicillin is high |
| B. | Mycelial mass increases |
| C. | pH increase |
| D. | Both (a) and (b) |
| Answer» E. | |
| 15. |
Preliminary clinical results with a humanized antibody against the interleukin-2 receptor have suggested the |
| A. | absence of human immune response against murine proteins (HAMA) response |
| B. | presence of HAMA response |
| C. | poor recognition of immunoglobulin, Ig constant regions |
| D. | all of the above |
| Answer» B. presence of HAMA response | |
| 16. |
Penicillin is recovered after fermentation as |
| A. | potassium penicillin |
| B. | calcium penicillin |
| C. | sodium penicillin |
| D. | penicillin only |
| Answer» B. calcium penicillin | |
| 17. |
Chlorotetracyclin is soluble in |
| A. | organic solvents |
| B. | water |
| C. | ether |
| D. | all of these |
| Answer» C. ether | |
| 18. |
At acidic pH, penicillin remains in |
| A. | aqueous phase |
| B. | solvent phase |
| C. | both (a) and (b) |
| D. | precipitates |
| Answer» C. both (a) and (b) | |
| 19. |
The antigen-specific lymphocytes can be immortalized by which of the following method? |
| A. | Transfection with tumor derived DNA |
| B. | Hybridization with a suitable lymphoid tumor cell |
| C. | Transformation following infection by Epstein-Barr virus (EB V) |
| D. | All of the above |
| Answer» E. | |
| 20. |
Which type of cell actually secrets antibodies? |
| A. | plasma cells |
| B. | T cells |
| C. | macrophages |
| D. | dendritic cells |
| Answer» B. T cells | |
| 21. |
A cytokine that stimulates the activity of B and T cells is |
| A. | lymphotoxin |
| B. | interlukin-2 |
| C. | interlukin-1 |
| D. | all of these |
| Answer» C. interlukin-1 | |
| 22. |
Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as |
| A. | source of nutrients |
| B. | carbon source |
| C. | antifoam agent |
| D. | both (a) and (c) |
| Answer» D. both (a) and (c) | |
| 23. |
pH of the fermentation medium for chlorotetracyclins is |
| A. | 5-6 |
| B. | 6-7 |
| C. | 7-8 |
| D. | 8-9 |
| Answer» B. 6-7 | |
| 24. |
Which of the following event occurs during third phase of growth of P chrysogenum? |
| A. | Concentration of antibiotic increases in the medium |
| B. | Autolysis of the medium starts |
| C. | Slight rise in pH due to liberation of ammonia |
| D. | All of the above |
| Answer» E. | |
| 25. |
Inoculum preparation for the fermentation medium for penicillin takes place on |
| A. | wheat seeds |
| B. | barley seeds |
| C. | rice seeds |
| D. | all of these |
| Answer» C. rice seeds | |
| 26. |
The approach (s), which is/are currently followed to produce human monoclonal antibodies, is/are known as |
| A. | transformation of antigen specific B lymphocytes (EBV) |
| B. | hybridization of 6-thioguanine-resistant human plasmacytoma with immune human lymphocytes |
| C. | combination of EB Vand hybridoma techniques |
| D. | all of these |
| Answer» E. | |
| 27. |
Media used for the production of chlorotetracyclin consists of |
| A. | sugar, corn steep liquor, CaCO3, (NH4)2SO4 and NH4 Cl |
| B. | corn steep liquor, CaCO3 and (NH4)2SO4 |
| C. | sugar, CaCO3 and (NH4)2SO4 |
| D. | corn steep liquor, CaCO3, (NH4)2SO4 and NH4 Cl |
| Answer» B. corn steep liquor, CaCO3 and (NH4)2SO4 | |
| 28. |
Which of the following organism(s) produce(s) tetracycline? |
| A. | Steptomyces aureofaciens |
| B. | Steptomyces ramosus |
| C. | Nocardia sulphurea |
| D. | All of these |
| Answer» E. | |
| 29. |
The Ig locus is about |
| A. | 2/3rd of all hybridomas |
| B. | l/3rd of all hybridomas |
| C. | l/2nd of all hybridomas |
| D. | 1/4th of all hybridomas |
| Answer» B. l/3rd of all hybridomas | |
| 30. |
Streptomycin is produced by |
| A. | S. griseus |
| B. | S. griseoflavus |
| C. | S. aerofaciens |
| D. | S. ramosus |
| Answer» B. S. griseoflavus | |
| 31. |
High yield of chlorotetracyclin requires |
| A. | no aeration |
| B. | continuous aeration |
| C. | aeration which does not affect the yield |
| D. | controlled aeration |
| Answer» C. aeration which does not affect the yield | |
| 32. |
The yield of the antibiotic depends upon |
| A. | pH of the medium |
| B. | age of the inoculum |
| C. | composition of the medium |
| D. | all of these |
| Answer» E. | |
| 33. |
Fermentation medium for oxytetracyclin (terramycin) consist of |
| A. | CSL, starch, (NH4)2 SO4, sodium chloride and CaCO3 |
| B. | CSL, (NH4)2 SO4, sodium chloride and CaCO3 |
| C. | CSL, starch,(NH4)2 SO4, ammonium chloride and CaCO3 |
| D. | CSL,(NH4)2 SO4, ammonium chloride and CaCO3 |
| Answer» B. CSL, (NH4)2 SO4, sodium chloride and CaCO3 | |
| 34. |
During which phase of growth of Penicillium chrysogenum maximum antibiotic production takes place |
| A. | during the first phase |
| B. | during the second phase |
| C. | during the third phase |
| D. | same in all the phases |
| Answer» C. during the third phase | |
| 35. |
TC cells are important in controlling |
| A. | virus infections |
| B. | allergy |
| C. | autoimmunity |
| D. | all of these |
| Answer» E. | |
| 36. |
An example of mosaic antigen is |
| A. | virus |
| B. | bacteria |
| C. | a hapten |
| D. | protein |
| Answer» B. bacteria | |
| 37. |
The hybrid cells can be propagated |
| A. | in tissue culture |
| B. | as ascites in peritoneal cavity of mice |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» D. none of these | |
| 38. |
In human B cells and T cells are matured in the |
| A. | bone marrow and thymus respectively |
| B. | lymph nodes and spleen respectively |
| C. | bursa and thymus respectively |
| D. | none of these |
| Answer» B. lymph nodes and spleen respectively | |
| 39. |
The primary B cell receptor is |
| A. | IgD |
| B. | IgG |
| C. | IgA |
| D. | IgE |
| Answer» B. IgG | |
| 40. |
T cells are the source of |
| A. | interleukin |
| B. | interferon |
| C. | lymphotoxin |
| D. | all of these |
| Answer» E. | |
| 41. |
It is highly valued if the lymphocytes derived from the lymph node or tonsil tend to undergo fusion at |
| A. | high frequencies |
| B. | moderate frequencies |
| C. | low frequencies |
| D. | at no frequency |
| Answer» B. moderate frequencies | |
| 42. |
In immuno-inflammatory diseases such as hemolytic anaemia, eczema etc., |
| A. | T8 cells are greatly reduced |
| B. | T8 cells are greatly increased |
| C. | T4 cells are greatly reduced |
| D. | T4 cells are greatly increased |
| Answer» B. T8 cells are greatly increased | |
| 43. |
Helper T cells assist in the functions of |
| A. | certain B cells |
| B. | certain T cells |
| C. | certain B cells and other T cells |
| D. | none of the above |
| Answer» D. none of the above | |
| 44. |
The EBV-hybridoma technique |
| A. | immortalizes the donor Bcells |
| B. | facilitates the proliferation of antigen specific B cells |
| C. | gives much higher hybridization frequencies |
| D. | all of the above |
| Answer» E. | |
| 45. |
Small simple molecules are |
| A. | poor antigens |
| B. | rich antigens |
| C. | moderate antigens |
| D. | heterophilic antigens |
| Answer» B. rich antigens | |