MCQOPTIONS
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MCQOPTIONS
This section includes 40 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of |
| A. | genetic engineering |
| B. | population genetics |
| C. | microbiology |
| D. | protein engineering |
| Answer» B. population genetics | |
| 2. |
The two chains of insulin can be separated by detaching it from β-galactosidase using |
| A. | hydrogen chloride |
| B. | cyanogen bromide |
| C. | peptidase |
| D. | protease |
| Answer» C. peptidase | |
| 3. |
How many nucleotides are there in Ss RNA molecule of picorna virus causing FMD? |
| A. | 1000 |
| B. | 5000 |
| C. | 8000 |
| D. | 10000 |
| Answer» D. 10000 | |
| 4. |
In genetic engineering, a chimera is |
| A. | an enzyme that links DNA molecules |
| B. | a plasmid that contains foreign DNA |
| C. | a virus that infects bacteria |
| D. | a fungi |
| Answer» C. a virus that infects bacteria | |
| 5. |
The two chains are joined together to constitute native insulin using |
| A. | sodium dissulphonate and sodium sulphite |
| B. | sodium dissulphonate and sodium sulphate |
| C. | sodium sulphate and sodium sulphite |
| D. | sodium sulphate only |
| Answer» B. sodium dissulphonate and sodium sulphate | |
| 6. |
Human insulin formed by recombinant DNA technology is known as |
| A. | H insulin |
| B. | R insulin |
| C. | humulin |
| D. | huinsulin |
| Answer» D. huinsulin | |
| 7. |
Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid? |
| A. | DNA polymerase |
| B. | Restriction endonuclease |
| C. | DNA ligase |
| D. | DNA helicase |
| Answer» D. DNA helicase | |
| 8. |
Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of |
| A. | β-Galactosidase |
| B. | galactokinase |
| C. | acid phosphatase |
| D. | glucokinase |
| Answer» B. galactokinase | |
| 9. |
Bacterial cells protect their own DNA from degradation by restriction endonucleases by |
| A. | methylating the DNA at the sites that the enzyme recognizes |
| B. | deleting all recognition sites from the genome |
| C. | not producing any restriction endonucleases |
| D. | having anti restriction endonucleases |
| Answer» B. deleting all recognition sites from the genome | |
| 10. |
Recombinant vaccine for Hepatitis B virus has been synthesized against which of the following antigen? |
| A. | Viral core antigen (HBcAg) |
| B. | Viral surface antigen (HBsAg) |
| C. | e antigen (HBeAg) |
| D. | All of the above |
| Answer» C. e antigen (HBeAg) | |
| 11. |
How is human insulin synthesized using recombinant DNA technology? |
| A. | By using chemically synthesized DNA sequences for the two chains separately |
| B. | By isolating DNA from the islets of Langerhans of pancreas |
| C. | By using cDNA for insulin |
| D. | By using chemically synthesized DNA sequences for the complete insulin protein |
| Answer» B. By isolating DNA from the islets of Langerhans of pancreas | |
| 12. |
The promoter used for cloning HBV surface antigen is |
| A. | galactokinase |
| B. | alcohol dehydrogenase 1 |
| C. | β-galactosidase |
| D. | polyhedrin |
| Answer» C. β-galactosidase | |
| 13. |
The gene coding for VP1 is cloned in |
| A. | pMB 9 |
| B. | pBR 322 |
| C. | pUC 18 |
| D. | pUC 19 |
| Answer» C. pUC 18 | |
| 14. |
The advantage of using DNA polymerases from thermophilic organisms in PCR is that |
| A. | the DNA polymerases of these bacteria are much faster than those from other organisms |
| B. | the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands |
| C. | the DNA polymerases of these bacteria never make mistakes while replicating DNA |
| D. | all of the above |
| Answer» C. the DNA polymerases of these bacteria never make mistakes while replicating DNA | |
| 15. |
Foot and mouth disease (FMD) of animals is caused by |
| A. | RNA virus |
| B. | DNA virus |
| C. | bacteria |
| D. | protozoa |
| Answer» B. DNA virus | |
| 16. |
Which type of restriction endonuclease cuts the DNA within the recognition site? |
| A. | Type I |
| B. | Type II |
| C. | Type III |
| D. | All of the these |
| Answer» C. Type III | |
| 17. |
Which of the following vector can maintain the largest fragment of foreign DNA? |
| A. | YAC |
| B. | Cosmid |
| C. | Plasmid |
| D. | Phage |
| Answer» B. Cosmid | |
| 18. |
Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are |
| A. | DNA ligase |
| B. | helicases |
| C. | restriction endonucleases |
| D. | DNA gyrase |
| Answer» D. DNA gyrase | |
| 19. |
Agrobacterium tumefaciens is |
| A. | a disease in humans that causes loss of sight |
| B. | a bacterium that can be used to introduce DNA into plants |
| C. | a fungi that is used to produce antibiotics in large amounts |
| D. | a disease in humans that causes loss of weight |
| Answer» C. a fungi that is used to produce antibiotics in large amounts | |
| 20. |
Recombinant vaccine for HBV was produced by cloning viral genes in |
| A. | plasmids |
| B. | cosmids |
| C. | autonomously replicating plasmid of yeast |
| D. | phagemids |
| Answer» D. phagemids | |
| 21. |
A genomic library is |
| A. | a database where the sequence of an organism's genome is stored |
| B. | a collection of many clones possessing different DNA fragments from the same organisms bound to vectors |
| C. | a book that describes how to isolate DNA from a particular organism |
| D. | a place where the information of the genetic organization of organisms are kept. |
| Answer» C. a book that describes how to isolate DNA from a particular organism | |
| 22. |
The first therapeutic product formed by means of recombinant DNA technology is |
| A. | human growth hormone |
| B. | insulin |
| C. | hepatitis B vaccine |
| D. | vaccine for foot and mouth disease |
| Answer» C. hepatitis B vaccine | |
| 23. |
Human growth hormone (hGH) is secreted by |
| A. | pitutary gland |
| B. | hypothalamus |
| C. | pancreas |
| D. | none of these |
| Answer» B. hypothalamus | |
| 24. |
Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as |
| A. | photoreactivation |
| B. | gel electrophoresis |
| C. | autoradiography |
| D. | blotting |
| Answer» C. autoradiography | |
| 25. |
For gene probes to be useful they must |
| A. | be large enough to contain gene-specific sequences |
| B. | be labeled in some manner to allow detection |
| C. | both (a) and (b) |
| D. | none of the above |
| Answer» D. none of the above | |
| 26. |
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as |
| A. | southern blotting |
| B. | northern blotting |
| C. | polymerase chain reaction |
| D. | DNA replication |
| Answer» D. DNA replication | |
| 27. |
In India first genetically engineered vaccine against HBV was developed by |
| A. | Ranbaxy Pvt Ltd |
| B. | Shantha Biotechnics Pvt Ltd |
| C. | Dabur Pvt Ltd |
| D. | Glaxo Pvt Ltd |
| Answer» C. Dabur Pvt Ltd | |
| 28. |
Which of the following is not commonly used as vector? |
| A. | Artificial chromosome |
| B. | Cosmid |
| C. | Fungi |
| D. | Plasmid |
| Answer» D. Plasmid | |
| 29. |
The Southern blotting technique depends on |
| A. | similarities between the sequences of probe DNA and experimental DNA |
| B. | similarities between the sequences of probe RNA and experimental RNA |
| C. | similarities between the sequences of probe protein and experimental protein |
| D. | the molecular mass of proteins |
| Answer» B. similarities between the sequences of probe RNA and experimental RNA | |
| 30. |
The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as |
| A. | laser |
| B. | DNA probe |
| C. | gene gun |
| D. | inoculating needle |
| Answer» D. inoculating needle | |
| 31. |
Which one of the following capsid protein of picorna virus is immunogenic in nature? |
| A. | VP1 |
| B. | VP2 |
| C. | VP3 |
| D. | VP4 |
| Answer» B. VP2 | |
| 32. |
An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as |
| A. | a chimera |
| B. | a transgenic animal |
| C. | a vector |
| D. | an enzyme that links DNA molecules |
| Answer» C. a vector | |
| 33. |
The recombinant hGH lacks |
| A. | terminal glutamine |
| B. | terminal lysine |
| C. | terminal methionine |
| D. | terminal arginine |
| Answer» D. terminal arginine | |
| 34. |
Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using |
| A. | BACS |
| B. | expression vectors |
| C. | YACS |
| D. | all of these |
| Answer» C. YACS | |
| 35. |
A short molecule containing 2-20 nucleotide is |
| A. | plasmid |
| B. | vector |
| C. | oligonucleotide |
| D. | mononucleotide |
| Answer» D. mononucleotide | |
| 36. |
Vectors are |
| A. | molecules that degrade nucleic acids |
| B. | molecules that help in replication |
| C. | molecules that are able to covalently bond to and carry foreign DNA into cells |
| D. | molecules that protect host cells from invasion by foreign DNA |
| Answer» D. molecules that protect host cells from invasion by foreign DNA | |
| 37. |
What is the normal role of restriction endonucleases in bacterial cells? |
| A. | To degrade the bacterial chromosome into small pieces during replication |
| B. | To degrade invading phage DNA |
| C. | To produce RNA primers for replication |
| D. | All of the above |
| Answer» C. To produce RNA primers for replication | |
| 38. |
Which of the following is obtained using processed mRNA molecules as a template? |
| A. | rDNA |
| B. | mDNA |
| C. | cDNA |
| D. | tDNA |
| Answer» D. tDNA | |
| 39. |
The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of |
| A. | genetic engineering |
| B. | population genetics |
| C. | microbiology |
| D. | protein engineering |
| Answer» B. population genetics | |
| 40. |
Electroporation is |
| A. | the process of separating charged molecules through a gel maintained in an electric field |
| B. | the process of combining foreign DNA to an electrically charged vector molecule |
| C. | the application of high voltage pulses |
| D. | the process of multiplication of the cells |
| Answer» D. the process of multiplication of the cells | |