MCQOPTIONS
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MCQOPTIONS
This section includes 51 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Two genes from a bacterium () and a cotton gene produce |
| A. | a plastic |
| B. | a vaccine against the bacterium |
| C. | a pharmaceutical product |
| D. | herbicide resistance |
| Answer» B. a vaccine against the bacterium | |
| 2. |
Plants derived sexually from the same plant are____ while those derived from somatic tissue from the same plant are____. |
| A. | identical, different |
| B. | different, also different |
| C. | different, identical |
| D. | plants cannot be derived from somatic tissue |
| Answer» D. plants cannot be derived from somatic tissue | |
| 3. |
Transgenic plants with increased tolerance to aluminum have been produced by making plants that |
| A. | secrete phytosiderophores into the soil |
| B. | make more metal-binding peptides like phytochelatins |
| C. | bind aluminum to the cell wall |
| D. | secrete citrate into the soil |
| Answer» E. | |
| 4. |
Plants containing genes encoding cytokines and blood clotting factors are used in |
| A. | nutrition improvement |
| B. | pharmaceutical production |
| C. | vaccine production |
| D. | textile production |
| Answer» C. vaccine production | |
| 5. |
The first transgenic plants expressing engineered foreign genes were tobacco plants produced by the use of |
| A. | <i>Agrobacterium tumefaciens</i> |
| B. | <i>Bacillus thuringiensis</i> |
| C. | <i>Arabidopsis thaliana</i> |
| D. | <i>Streptomyces hygroscopicus</i> |
| Answer» B. <i>Bacillus thuringiensis</i> | |
| 6. |
Which of the agricultural challenges below cannot be solved with transgenic techniques? |
| A. | Crops are damaged by frost |
| B. | Crops are killed by a virus |
| C. | Public concern about safety of synthetic pesticides |
| D. | Public preference for organic vegetables |
| Answer» E. | |
| 7. |
Transplastomics |
| A. | targets genes in the chloroplast |
| B. | provides exceptionally low yields of protein products |
| C. | produces genes that are released in pollen |
| D. | offers little opportunity for practical use |
| Answer» B. provides exceptionally low yields of protein products | |
| 8. |
Which of the following statements is true regarding genomics? |
| A. | Plant genomics lags behind similar efforts in animals and microorganisms |
| B. | Researchers can make use of genomic information even if the entire genome of an organism is not known |
| C. | Researchers are busy in trying to determine the genome of the potato plant |
| D. | All of the above |
| Answer» E. | |
| 9. |
Which of the following gene is responsible for resistance against chilling? |
| A. | Glycerol 1 phosphate acyl transferase |
| B. | Polygalactouranase |
| C. | ACC deaminase |
| D. | Sucrose phosphate synthase gene |
| Answer» B. Polygalactouranase | |
| 10. |
Which of the following gene detoxifies herbicide phosphinothricin? |
| A. | Nitrilase |
| B. | Glutathione S-transferase (GST) |
| C. | Phosphinothricin acetyl transferase |
| D. | All of these |
| Answer» D. All of these | |
| 11. |
The controversy regarding the use of Bt corn is that it |
| A. | is potentially harmful to monarch butterflies |
| B. | is a potential allergen to humans |
| C. | both (a) and (b) |
| D. | can contaminate groundwater |
| Answer» D. can contaminate groundwater | |
| 12. |
Starch content of potatoes can be increased by using a bacterial gene, known as |
| A. | sucrose phosphate synthase gene |
| B. | ADP glucose pyrophosphorylase gene |
| C. | polygalactouranase gene |
| D. | none of the above |
| Answer» C. polygalactouranase gene | |
| 13. |
In a __________ protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area. |
| A. | microcosm establishment |
| B. | mibridization |
| C. | bioremediation |
| D. | rhizosecretion |
| Answer» D. rhizosecretion | |
| 14. |
Resistance to sulphonyl urea compounds in transgenic tobacco plant has been developed by the transfer of |
| A. | gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase) |
| B. | gene for ALS (acetolactate synthase) |
| C. | gene for GS (glutamine synthase) |
| D. | any of the above |
| Answer» C. gene for GS (glutamine synthase) | |
| 15. |
Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of |
| A. | gene for EPSPS(5-enol-pyruvyl shikimat 3 phosphate synthase) |
| B. | gene for ALS (acetolactate synthase) |
| C. | gene for GS (glutamine synthase) |
| D. | any of the above |
| Answer» B. gene for ALS (acetolactate synthase) | |
| 16. |
Which of the following compounds has been produced in transgenic plants to improve tolerance to salt stress and water deficit? |
| A. | Sucrose |
| B. | Mannitol |
| C. | Nicotine |
| D. | Octopine |
| Answer» C. Nicotine | |
| 17. |
Expression of antisense RNA in transgenic plants is a general method used to |
| A. | activate the expression of all genes in a biochemical pathway |
| B. | eliminate the expression of all genes in a biochemical pathway |
| C. | block the expression of virus coat protein genes |
| D. | reduce or eliminate the expression of individual genes |
| Answer» E. | |
| 18. |
Resistance to L-phosphinothrium in tobacco plants is developed by the transfer of |
| A. | gene for EPSPS (5-enol-pyruvyl shikimat 3 phosphate synthase) |
| B. | gene for ALS (acetolactate synthase) |
| C. | gene for GS (glutamine synthase) |
| D. | any of the above |
| Answer» D. any of the above | |
| 19. |
A __________ is a small laboratory set up to simulate conditions of a particular environment. |
| A. | microcosm |
| B. | mibridization |
| C. | bioremediation |
| D. | rhizosecretion |
| Answer» B. mibridization | |
| 20. |
The roots of a plant are converted into drug-producing structures in a process called |
| A. | microcosm establishment |
| B. | mibridization |
| C. | bioremediation |
| D. | rhizosecretion |
| Answer» E. | |
| 21. |
Which cell-based technology endows a cell with increased ability to harness energy? |
| A. | Chlybridization |
| B. | Cybridization |
| C. | Mibridization |
| D. | Protoplast fusion |
| Answer» B. Cybridization | |
| 22. |
The delayed ripening tomato was created by a biotechnologist who __________ a gene. |
| A. | altered |
| B. | silenced |
| C. | replaced |
| D. | relocated |
| Answer» C. replaced | |
| 23. |
Antisense technology |
| A. | selectively blocks expression of a gene |
| B. | combines genetic material from different species |
| C. | combines organelles and cells |
| D. | alters or transfers cells |
| Answer» B. combines genetic material from different species | |
| 24. |
Antisense transgenic plants produced fruit that softened |
| A. | more slowly than the normal fruit |
| B. | more rapidly than the normal fruit |
| C. | as the normal fruits |
| D. | none of these |
| Answer» B. more rapidly than the normal fruit | |
| 25. |
ACC demainase gene is responsible for |
| A. | synthesis for ethylene |
| B. | degradation of ACC an immediate precursor to ethylene |
| C. | both (a) and (b) |
| D. | synthesis of polygalactouranase |
| Answer» C. both (a) and (b) | |
| 26. |
Which of the following genes can be used for making resistances against viral infection? |
| A. | Genes for capsid protein |
| B. | Gene for nucleocapsid protein |
| C. | Satellite RNA |
| D. | All of these |
| Answer» E. | |
| 27. |
Cross protection against viruses in transgenic plants can be obtained by |
| A. | inoculating the susceptible strain of a crop with a mild strain of a virus that helps in developing resistance against more virulent strain |
| B. | inoculating the susceptible strain with the coat proteins of virulent strain |
| C. | inoculating the susceptible strain with genes of nucleocapsid |
| D. | any of the above |
| Answer» B. inoculating the susceptible strain with the coat proteins of virulent strain | |
| 28. |
The first field tests were conducted with which of the following genetically altered organism? |
| A. | bt Corn |
| B. | Vaccinia virus containing a gene from the rabies virus |
| C. | The flavrsavr tomato |
| D. | Strawberry seedlings sprayed with ice-minus bacteria |
| Answer» E. | |
| 29. |
Which of the following self-pollinating plant/(s) tend to be homozygous? |
| A. | Peas |
| B. | Tomato |
| C. | Peach |
| D. | All of these |
| Answer» E. | |
| 30. |
Which cell-based plant technology involves the combining of two cells without cell walls from different species? |
| A. | Clonal propagation |
| B. | Cybridization |
| C. | Protoplast fusion |
| D. | Mutant selection |
| Answer» D. Mutant selection | |
| 31. |
A naturally occurring variant, possessing characteristics of interest, is identified. This plant is selectively bred. This is an example of |
| A. | traditional plant breeding |
| B. | transgenic technology |
| C. | mutant selection |
| D. | none of these |
| Answer» B. transgenic technology | |
| 32. |
Transgenic plants |
| A. | contain foreign genes in their cells |
| B. | are used to produce human antibodies |
| C. | both (a) and (b) |
| D. | are plants that differ in geographical locations |
| Answer» D. are plants that differ in geographical locations | |
| 33. |
Low temperatures induce the expression of many cold-induced genes. Transgenic plants with improved cold tolerance have been produced by |
| A. | expressing the protein that activates expression of these genes all the time in plants |
| B. | cooling plants using the Peltier effect |
| C. | expressing a gene for production of antifreeze (ethylene glycol) in plants |
| D. | increasing evaporative cooling from leaf surfaces |
| Answer» B. cooling plants using the Peltier effect | |
| 34. |
Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule? |
| A. | AUUAAGGC |
| B. | UAAUUCCG |
| C. | CGGAATTA |
| D. | TAATTCCG |
| Answer» B. UAAUUCCG | |
| 35. |
If the goal were to create a plant resistant to an insecticide, which cell-based plant technology would be most effective? |
| A. | Clonal propagation |
| B. | Cybridization |
| C. | Protoplast fusion |
| D. | Mutant selection |
| Answer» E. | |
| 36. |
What are the various disadvantages of cross protection? |
| A. | Possibility of mutations in inducing mild virus strain |
| B. | Possibility of synergism between inducing virus and other unrelated virus |
| C. | Possibility of unnecessary spread of mild virus |
| D. | All of the above |
| Answer» E. | |
| 37. |
In transgenic plants that have been manipulated to express patho-genesis-related (PR) proteins all the time it has been shown that |
| A. | these provide no protection at all against pathogens |
| B. | expression of one PR protein provides high levels of protection against many pathogens |
| C. | expression of two PR proteins provides some protection against a few pathogens |
| D. | these proteins trigger the production of other antimicrobial defenses |
| Answer» E. | |
| 38. |
Which of the following gene detoxify herbicide bronoxynil? |
| A. | Nitrilase |
| B. | Glutathione S-transferase (GST) |
| C. | Phosphinothricin acetyl transferase |
| D. | All of these |
| Answer» B. Glutathione S-transferase (GST) | |
| 39. |
For developing herbicide resistance in transgenic plants which of the following approach/(es) is/are used? |
| A. | Target molecule is made insensitive to herbicide |
| B. | Target protein is overproduced |
| C. | A pathway should be introduced that detoxify the herbicide |
| D. | All of the above |
| Answer» E. | |
| 40. |
Which of the following is not a characteristic of a transgenic crop? |
| A. | herbicide resistance |
| B. | Bt insect resistance toxin |
| C. | increased methionine content |
| D. | none of these |
| Answer» E. | |
| 41. |
Reducing the expression of pectin methylesterase (PME) has resulted in |
| A. | plants with resistance to bacteria and fungi |
| B. | tomato juice that has higher viscosity |
| C. | tomato fruit that last longer before they become rotten |
| D. | tomato fruit that will not ripen |
| Answer» C. tomato fruit that last longer before they become rotten | |
| 42. |
Which of the following gene is transferred to plants that detoxify the herbicide atrazine? |
| A. | Nitrilase |
| B. | Glutathione S-transferase (GST) |
| C. | Phosphinothrium acetyl transferase |
| D. | All of these |
| Answer» C. Phosphinothrium acetyl transferase | |
| 43. |
Two genes from a bacterium (Alcaligenes eutrophus) and a cotton gene produce |
| A. | a plastic |
| B. | a vaccine against the bacterium |
| C. | a pharmaceutical product |
| D. | herbicide resistance |
| Answer» B. a vaccine against the bacterium | |
| 44. |
Bruise resistant tomatoes have been developed by the expression of antisense RNA against |
| A. | glycerol 1 phosphate acyl transferase |
| B. | polygalactouranase |
| C. | ACC deaminase |
| D. | Sucrose phosphate synthase gene |
| Answer» C. ACC deaminase | |
| 45. |
Which of the following has been widely used to provide resistance against plant viruses? |
| A. | Virus resistance genes from bacteria |
| B. | Expression of virus coat protein genes in transgenic plants |
| C. | Expression of anti-virus genes in vectors that transmit viruses |
| D. | Expression of ribonuclease (RNase) genes in host plants |
| Answer» C. Expression of anti-virus genes in vectors that transmit viruses | |
| 46. |
Insect resistance in the transgenic plant has been achieved by |
| A. | transferring genes for Bt toxins |
| B. | transferring genes for protease inhibitors |
| C. | transferring genes for other insecticidal secondary metabolities |
| D. | all of the above |
| Answer» E. | |
| 47. |
Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato? |
| A. | ACC (1-aminocyclopropane-1-carboxylic acid) |
| B. | SAM (S-adenosylmethionine) |
| C. | Both (a) and (b) |
| D. | AOA (aminooxyacetic acid) |
| Answer» D. AOA (aminooxyacetic acid) | |
| 48. |
Which of the following metabolites are implicated in stress tolerance? |
| A. | Proline |
| B. | Betaines |
| C. | Both (a) and (b) |
| D. | Citrate |
| Answer» D. Citrate | |
| 49. |
Which of the following dies from Ti plasmid infection? |
| A. | Rice |
| B. | Corn |
| C. | Sorghum |
| D. | All of these |
| Answer» E. | |
| 50. |
Tomatoes exhibiting delayed ripening express antisense RNA against |
| A. | glycerol 1 phosphate acyl transferase |
| B. | polygalactouranase |
| C. | ACC deaminase |
| D. | sucrose phosphate synthase gene |
| Answer» D. sucrose phosphate synthase gene | |