23 + Mcqs in Recombinant DNA Page 1 McqOptions

1.	A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
A.	southern blotting
B.	northern blotting
C.	polymerase chain reaction
D.	DNA replication
Answer» D. DNA replication

Discussion

2.	A genomic library is
A.	a database where the sequence of an organism's genome is stored
B.	a collection of many clones possessing different DNA fragments from the same organisms bound to vectors
C.	a book that describes how to isolate DNA from a particular organism
D.	a place where the information of the genetic organization of organisms are kept.
Answer» C. a book that describes how to isolate DNA from a particular organism

Discussion

3.	The advantage of using DNA polymerases from thermophilic organisms in PCR is that
A.	the DNA polymerases of these bacteria are much faster than those from other organisms
B.	the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
C.	the DNA polymerases of these bacteria never make mistakes while replicating DNA
D.	all of the above
Answer» C. the DNA polymerases of these bacteria never make mistakes while replicating DNA

Discussion

4.	An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as
A.	a chimera
B.	a transgenic animal
C.	a vector
D.	an enzyme that links DNA molecules
Answer» C. a vector

Discussion

5.	What is the normal role of restriction endonucleases in bacterial cells?
A.	To degrade the bacterial chromosome into small pieces during replication
B.	To degrade invading phage DNA
C.	To produce RNA primers for replication
D.	All of the above
Answer» C. To produce RNA primers for replication

Discussion

6.	Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
A.	DNA polymerase
B.	Restriction endonuclease
C.	DNA ligase
D.	DNA helicase
Answer» D. DNA helicase

Discussion

7.	Which of the following is not commonly used as vector?
A.	Artificial chromosome
B.	Cosmid
C.	Fungi
D.	Plasmid
Answer» D. Plasmid

Discussion

8.	Bacterial cells protect their own DNA from degradation by restriction endonucleases by
A.	methylating the DNA at the sites that the enzyme recognizes
B.	deleting all recognition sites from the genome
C.	not producing any restriction endonucleases
D.	having anti restriction endonucleases
Answer» B. deleting all recognition sites from the genome

Discussion

9.	Which type of restriction endonuclease cuts the DNA within the recognition site?
A.	Type I
B.	Type II
C.	Type III
D.	All of the these
Answer» C. Type III

Discussion

10.	The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of
A.	genetic engineering
B.	population genetics
C.	microbiology
D.	protein engineering
Answer» B. population genetics

Discussion

11.	A short molecule containing 2-20 nucleotide is
A.	plasmid
B.	vector
C.	oligonucleotide
D.	mononucleotide
Answer» D. mononucleotide

Discussion

12.	Electroporation is
A.	the process of separating charged molecules through a gel maintained in an electric field
B.	the process of combining foreign DNA to an electrically charged vector molecule
C.	the application of high voltage pulses
D.	the process of multiplication of the cells
Answer» D. the process of multiplication of the cells

Discussion

13.	Which of the following is obtained using processed mRNA molecules as a template?
A.	rDNA
B.	mDNA
C.	cDNA
D.	tDNA
Answer» D. tDNA

Discussion

14.	The Southern blotting technique depends on
A.	similarities between the sequences of probe DNA and experimental DNA
B.	similarities between the sequences of probe RNA and experimental RNA
C.	similarities between the sequences of probe protein and experimental protein
D.	the molecular mass of proteins
Answer» B. similarities between the sequences of probe RNA and experimental RNA

Discussion

15.	Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using
A.	BACS
B.	expression vectors
C.	YACS
D.	all of these
Answer» C. YACS

Discussion

16.	Agrobacterium tumefaciens is
A.	a disease in humans that causes loss of sight
B.	a bacterium that can be used to introduce DNA into plants
C.	a fungi that is used to produce antibiotics in large amounts
D.	a disease in humans that causes loss of weight
Answer» C. a fungi that is used to produce antibiotics in large amounts

Discussion

17.	In genetic engineering, a chimera is
A.	an enzyme that links DNA molecules
B.	a plasmid that contains foreign DNA
C.	a virus that infects bacteria
D.	a fungi
Answer» C. a virus that infects bacteria

Discussion

18.	Which of the following vector can maintain the largest fragment of foreign DNA?
A.	YAC
B.	Cosmid
C.	Plasmid
D.	Phage
Answer» B. Cosmid

Discussion

19.	For gene probes to be useful they must
A.	be large enough to contain gene-specific sequences
B.	be labeled in some manner to allow detection
C.	both (a) and (b)
D.	none of the above
Answer» D. none of the above

Discussion

20.	Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as
A.	photoreactivation
B.	gel electrophoresis
C.	autoradiography
D.	blotting
Answer» C. autoradiography

Discussion

21.	Vectors are
A.	molecules that degrade nucleic acids
B.	molecules that help in replication
C.	molecules that are able to covalently bond to and carry foreign DNA into cells
D.	molecules that protect host cells from invasion by foreign DNA
Answer» D. molecules that protect host cells from invasion by foreign DNA

Discussion

22.	Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are
A.	DNA ligase
B.	helicases
C.	restriction endonucleases
D.	DNA gyrase
Answer» D. DNA gyrase

Discussion

23.	The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as
A.	laser
B.	DNA probe
C.	gene gun
D.	inoculating needle
Answer» D. inoculating needle

Discussion

Explore topic-wise MCQs in Biotechnology.

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

A genomic library is

The advantage of using DNA polymerases from thermophilic organisms in PCR is that

An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

What is the normal role of restriction endonucleases in bacterial cells?

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

Which of the following is not commonly used as vector?

Bacterial cells protect their own DNA from degradation by restriction endonucleases by

Which type of restriction endonuclease cuts the DNA within the recognition site?

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

A short molecule containing 2-20 nucleotide is

Electroporation is

Which of the following is obtained using processed mRNA molecules as a template?

The Southern blotting technique depends on

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

Agrobacterium tumefaciens is

In genetic engineering, a chimera is

Which of the following vector can maintain the largest fragment of foreign DNA?

For gene probes to be useful they must

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

Vectors are

Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as