MCQOPTIONS

Explore topic-wise MCQs in Biotechnology.

This section includes 19 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.

1.	A() __________ is an excised piece of leaf or stem tissue used in micropropagation.
A.	microshoot
B.	medium
C.	explant
D.	scion
Answer» D. scion

2.	Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
A.	cellulotyic enzymes
B.	pectolytic enzymes
C.	both cellulotyic and pectolytic enzymes
D.	proteolytic enzymes
Answer» D. proteolytic enzymes

3.	Subculturing is similar to propagation by cuttings because
A.	it separates multiple microshoots and places them in a medium
B.	it uses scions to produce new microshoots
C.	they both use in vitro growing conditions
D.	all of the above
Answer» B. it uses scions to produce new microshoots

4.	Organogenesis is
A.	formation of callus tissue
B.	formation of root and shoots on callus tissue
C.	both (a) and (b)
D.	genesis of organs
Answer» C. both (a) and (b)

5.	Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
A.	Nurse medium
B.	Nurse or feeder culture
C.	Both (a) and (b)
D.	None of these
Answer» D. None of these

6.	Agrobacterium based gene transfer is efficient
A.	only with dicots
B.	only with monocots
C.	with both monocots and dicots
D.	with majority monocots and few dicots
Answer» B. only with monocots

7.	The ability of the component cells of callus to form a whole plant is known as
A.	redifferentiation
B.	dedifferentiation
C.	either (a) or (b)
D.	none of these
Answer» B. dedifferentiation

8.	What is/are the benefit(s) of micropropagation or clonal propagation?
A.	Rapid multiplication of superior clones
B.	Multiplication of disease free plants
C.	Multiplication of sexually derived sterile hybrids
D.	All of the above
Answer» E.

9.	Cell fusion method includes the preparation of large number of
A.	plant cells stripped of their cell wall
B.	single plant cell stripped of their cell wall
C.	plant cells with cell wall
D.	cells from different species
Answer» C. plant cells with cell wall

10.	Which breeding method uses a chemical to strip the cell wall of plant cells of two sexually incompatible species?
A.	Mass selection
B.	Protoplast fusion
C.	Transformation
D.	Transpiration
Answer» C. Transformation

11.	Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
A.	Multiplication of sexually derived sterile hybrids
B.	Less multiplication of disease free plants
C.	Storage and transportation of propogates
D.	Both (b) and (c)
Answer» D. Both (b) and (c)

12.	Which method of plant propagation involves the use of girdling?
A.	Grafting
B.	Cuttings
C.	Layering
D.	Micropropagation
Answer» D. Micropropagation

13.	A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.
A.	microshoot
B.	medium
C.	explant
D.	scion
Answer» D. scion

14.	The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as
A.	redifferentiation
B.	dedifferentiation
C.	either (a) or (b)
D.	none of these
Answer» C. either (a) or (b)

15.	When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?
A.	2-5 days
B.	5-10 days
C.	10-15 days
D.	15-17 days
Answer» C. 10-15 days

16.	What is meant by 'Organ culture' ?
A.	Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
B.	Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
C.	Cultivation of organs in a laboratory through the synthesis of tissues
D.	The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
Answer» B. Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal

17.	Protoplasts are the cells devoid of
A.	cell membrane
B.	cell wall
C.	both cell wall and cell membrane
D.	none of these
Answer» C. both cell wall and cell membrane

18.	In a callus culture
A.	increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation
B.	increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
C.	auxins and cytokinins are not required
D.	only auxin is required for root and shoot formation
Answer» B. increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation

19.	Cellular totipotency is the property of
A.	plants
B.	animals
C.	bacteria
D.	all of these
Answer» B. animals