MCQOPTIONS
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MCQOPTIONS
This section includes 20 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which is the most common carbon source used in the plant cell culture media? |
| A. | Sucrose |
| B. | Glucose |
| C. | Fructose |
| D. | Maltose |
| Answer» B. Glucose | |
| 2. |
Which of the following is an ethylene biosynthesis inhibitor? |
| A. | Citric acid |
| B. | Succinic acid |
| C. | Activated charcoal |
| D. | Silver thiosulphate |
| Answer» E. | |
| 3. |
Which of the following growth regulator is added for short initiation during plant regeneration from callus? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» C. Gibberellins | |
| 4. |
To maintain the pH of the culture |
| A. | organic acid such as citric, fumaric, malic and succinic acid is used |
| B. | synthetic buffers such as Tris, MES or HEPS are used |
| C. | both (a) and (b) |
| D. | ammonium salts are used |
| Answer» D. ammonium salts are used | |
| 5. |
Which of the following growth regulator is used to stimulate embryo or shoot development? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» D. Brassinosteroids | |
| 6. |
Which of the following growth regulator cause plant cells to grow? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» B. Cytokinins | |
| 7. |
In plant cell culture media, auxins and cytokinins are used in the range of |
| A. | l-50 M |
| B. | 50-100 M |
| C. | 100-125 M |
| D. | more than 125 M |
| Answer» B. 50-100 M | |
| 8. |
Which of the following is not true about nurse or conditioned medium? |
| A. | It is liquid removed from the suspension of fast growing cells |
| B. | It contains uncharacterized growth factor released by growing cells |
| C. | It is used in the culture of regenerating protoplast |
| D. | it is removed aseptically from the culture and is autoclaved before use |
| Answer» E. | |
| 9. |
What is 'nurse' or conditioned medium? |
| A. | It is the media full of growth factors used for the growth of cells |
| B. | It is the medium added to nurse the callus culture |
| C. | Both (a) and (b) |
| D. | It is the liquid medium removed from the suspension of fast growing cells |
| Answer» E. | |
| 10. |
What are the macronutrients used in plant cell culture medium? |
| A. | N, P, K, S, Na |
| B. | N, P, K, Ca, Cl |
| C. | N, P, K, S, Ca |
| D. | N, P, Ca, Na, Cl |
| Answer» D. N, P, Ca, Na, Cl | |
| 11. |
Which of the following is not a cytokinin? |
| A. | 2,4-dichlorophenoxyacetic acid |
| B. | 6 benzylaminopurine |
| C. | Zeatin |
| D. | Kinetin |
| Answer» B. 6 benzylaminopurine | |
| 12. |
Which of the following is not an auxin? |
| A. | Indole acetic acid (IAA) |
| B. | Naphthalenacetic acid (NAA) |
| C. | zeatin |
| D. | Indole butyric acid |
| Answer» D. Indole butyric acid | |
| 13. |
Concentration of sucrose generally used in plant cell culture media is |
| A. | 10-15 g/l |
| B. | 20-30 g/l |
| C. | 40-50 g/l |
| D. | 60-70 g/l |
| Answer» C. 40-50 g/l | |
| 14. |
Which is/are the naturally occurring plant auxins? |
| A. | Indole acetic acid (IAA) |
| B. | Naphthalenacetic acid (NAA) |
| C. | 2,4-dichlorophenoxyacetic acid |
| D. | All of the above |
| Answer» B. Naphthalenacetic acid (NAA) | |
| 15. |
Very high sugar concentration (40-100 g/l) have been used |
| A. | in specialized secondary metabolite production |
| B. | to adjust the osmotic potential of the media in short term treatment for regeneration |
| C. | Both (a) and (b) |
| D. | none of these |
| Answer» D. none of these | |
| 16. |
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media |
| A. | utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise |
| B. | utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise |
| C. | utilization of both ammonium and nitrate result in rise in pH |
| D. | utilization of both ammonium and nitrate result in drop in pH |
| Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | |
| 17. |
Which is/are the disadvantage/(s) of using IAA in plant cell culture media? |
| A. | It is unstable in solution |
| B. | Gets easily oxidized |
| C. | Conjugated to inactive form by plant cells |
| D. | All of the above |
| Answer» E. | |
| 18. |
Which of the following growth regulator promote cell division? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» C. Gibberellins | |
| 19. |
Neutralized activated charcoal is occasionally added to young regenerating cultures to |
| A. | remove toxic phenolics produced by the stressed plant cell |
| B. | help to remove plants growth regulators introduced at an earlier stage |
| C. | both (a) and (b) |
| D. | maintain the pH of the medium |
| Answer» D. maintain the pH of the medium | |
| 20. |
Silver thiosulphate is added to culture medium as it helps to |
| A. | maintain the pH |
| B. | remove toxic phenolics from plant cells |
| C. | prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. |
| D. | all of the above |
| Answer» D. all of the above | |