MCQOPTIONS
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MCQOPTIONS
This section includes 33 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco? |
| A. | Acetosyringone |
| B. | -hydroxy syringone |
| C. | Both (a) and (b) |
| D. | None of these |
| Answer» D. None of these | |
| 2. |
Liposomes mediated gene transfer in plants involves |
| A. | plasmid DNA enclosed in a lipid bag |
| B. | fusion of liposomes with protoplast |
| C. | use of polyethylene glycol (PEG) |
| D. | all of the above |
| Answer» E. | |
| 3. |
Which of the following are used as selection marker for the cells transformed with Agrobacterium? |
| A. | Neomycin phosphotransferase |
| B. | Streptomycin phosphotransferase |
| C. | Hygromycin phosphotransferase |
| D. | any of the above |
| Answer» E. | |
| 4. |
Vir genes required for the T-DNA transfer and processing are located |
| A. | on the T-DNA |
| B. | outside the T-DNA region |
| C. | on the plant genome |
| D. | none of these |
| Answer» C. on the plant genome | |
| 5. |
Microprojectile method of gene transfer in plants involves delivery of DNA |
| A. | with the help of micromanipulator |
| B. | with the help of bolistics |
| C. | with the help of needles |
| D. | any of the above |
| Answer» C. with the help of needles | |
| 6. |
Integrated octopine T DNA occurs as |
| A. | single segment |
| B. | two segments |
| C. | three segments |
| D. | four segments |
| Answer» C. three segments | |
| 7. |
The transfer of intermediate vectors into Agrobacterium are brought about by |
| A. | transformation |
| B. | biparental mating |
| C. | triparental mating |
| D. | transduction |
| Answer» D. transduction | |
| 8. |
Microinjection involves |
| A. | injection of large amount of DNA |
| B. | injection of DNA into bigger cells |
| C. | injection with needle having diameter greater than cell diameter |
| D. | all of the above |
| Answer» D. all of the above | |
| 9. |
The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as |
| A. | cos site |
| B. | flanking sequences |
| C. | border sequences |
| D. | transfer sequences |
| Answer» D. transfer sequences | |
| 10. |
The left segment of octopine T-DNA (TL) is necessary for |
| A. | enzymes for agropine biosynthesis |
| B. | tumour formation |
| C. | conjugative transfer |
| D. | all of these |
| Answer» C. conjugative transfer | |
| 11. |
In the liposome mediated gene transfer in plants, nucleic acids are |
| A. | protected from nuclease digestion |
| B. | stable in liposomes |
| C. | both (a) and (b) |
| D. | not stable in liposomes |
| Answer» D. not stable in liposomes | |
| 12. |
Co-integrating transformation vectors must include a region of homology in |
| A. | the vector plasmid |
| B. | the Ti-plasmid |
| C. | between vector plasmid and Ti-plasmid |
| D. | none of these |
| Answer» D. none of these | |
| 13. |
Crown gall tissue |
| A. | can be cultivated in vitro in absence of bacteria |
| B. | retains tumorous properties when cultivated |
| C. | both (a) and (b) |
| D. | shows tumorous properties only in presence of bacteria |
| Answer» D. shows tumorous properties only in presence of bacteria | |
| 14. |
On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of |
| A. | 12 bp |
| B. | 20 bp |
| C. | 25 bp |
| D. | 30 bp |
| Answer» D. 30 bp | |
| 15. |
Agrobacterium tumefaciens is a |
| A. | gram (+) bacteria |
| B. | gram (-) bacteria |
| C. | a fungi |
| D. | a yeast |
| Answer» C. a fungi | |
| 16. |
Opines are |
| A. | amino acid derivatives found in tumor tissues |
| B. | amino acid derivatives found in normal tissues |
| C. | amino acid derivatives found in both normal as well as tumor tissues |
| D. | none of the above |
| Answer» B. amino acid derivatives found in normal tissues | |
| 17. |
Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into |
| A. | pBR 322 based plasmid vector |
| B. | pRK 2013 |
| C. | pRN 3 |
| D. | all of these |
| Answer» B. pRK 2013 | |
| 18. |
Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast? |
| A. | It is carried out with the help of micromanipulator |
| B. | The recipient cells are immobilized on artificial support or artificially bound to substarate |
| C. | It employs needle with diameter greater than cell diameter |
| D. | all of the above |
| Answer» D. all of the above | |
| 19. |
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain |
| A. | one set of sequence necessary for gene transfer |
| B. | two sets of sequence necessary for gene transfer |
| C. | three sets of sequence necessary for gene transfer |
| D. | four sets of sequence necessary for gene transfer |
| Answer» C. three sets of sequence necessary for gene transfer | |
| 20. |
Opines that are present in crown gall tumour include |
| A. | octopine |
| B. | nopaline |
| C. | agropine |
| D. | all of these |
| Answer» E. | |
| 21. |
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid? |
| A. | pRK 2013 |
| B. | pRN 3 |
| C. | either (a) or (b) |
| D. | none of these |
| Answer» D. none of these | |
| 22. |
Which of the following is not true about the direct repeats flanking T-DNA? |
| A. | They are conserved between nopaline and octopine Ti-plasmids |
| B. | These repeats are transferred intact to the plant genome |
| C. | These are important in integration mechanism |
| D. | all of the above |
| Answer» C. These are important in integration mechanism | |
| 23. |
Virulence trait of Agrobacterium tumefaciens is borne on |
| A. | chromosomal DNA |
| B. | tumour inducing plasmid DNA |
| C. | both chromosomal and plasmid DNA |
| D. | cryptic plasmid DNA |
| Answer» C. both chromosomal and plasmid DNA | |
| 24. |
The size of the virulent plasmid of Agrobacterium tumefaciens is |
| A. | 40-80 kb |
| B. | 80-120 kb |
| C. | 140-235 kb |
| D. | >235 kb |
| Answer» D. >235 kb | |
| 25. |
Which of the following is true about T DNA? |
| A. | Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA |
| B. | Integration of T DNA occurs only at one specific sites in the plant nuclear DNA |
| C. | Integration of T DNA occurs at two specific sites in the plant nuclear DNA |
| D. | Integration of T DNA occurs at one site that may be random in the plant nuclear DNA |
| Answer» B. Integration of T DNA occurs only at one specific sites in the plant nuclear DNA | |
| 26. |
Which technique is used to introduce genes into dicots? |
| A. | electroporation |
| B. | particle acceleration |
| C. | microinjection |
| D. | Ti plasmid infection |
| Answer» E. | |
| 27. |
Integrated nopaline T-DNA occurs as |
| A. | single segment |
| B. | two segments |
| C. | three segments |
| D. | four segments |
| Answer» B. two segments | |
| 28. |
Advantage of microprojectile method over microinjection method for gene transfer in plants include |
| A. | intact cells are used |
| B. | method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall |
| C. | gene can be transferred to many cells simultaneously |
| D. | all of the above |
| Answer» E. | |
| 29. |
The right segment of octopine T-DNA (TR) is necessary for |
| A. | enzymes for agropine biosynthesis |
| B. | tumour formation |
| C. | conjugative transfer |
| D. | all of these |
| Answer» B. tumour formation | |
| 30. |
Direct DNA uptake by protoplasts can be stimulated by |
| A. | polyethylene glycol (PEG) |
| B. | decanal |
| C. | luciferin |
| D. | all of these |
| Answer» B. decanal | |
| 31. |
Plant transformation vectors based on Agrobacterium can generally be divided into |
| A. | two vectors |
| B. | four vectors |
| C. | six vectors |
| D. | eight vectors |
| Answer» B. four vectors | |
| 32. |
In a plant tumour cell |
| A. | complete Ti plasmid is incorporated in plant nuclear DNA |
| B. | different parts of the Ti-plasmid are incorporated |
| C. | only a small specific segment of callus T DNA is incorporated |
| D. | may vary from plant to plant |
| Answer» D. may vary from plant to plant | |
| 33. |
Which of the following is true about Agrobacterium tumefaciens? |
| A. | It causes crown gall disease of plants |
| B. | It infects gymosperms |
| C. | It infects dicotyledonous angiosperms |
| D. | All of the above |
| Answer» E. | |