MCQOPTIONS
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MCQOPTIONS
This section includes 29 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Cryptic plasmids |
| A. | do not exhibit any phenotypic trait |
| B. | exhibit many phenotypic traits |
| C. | exhibit one phenotypic traits |
| D. | exhibit antibiotic resistance |
| Answer» B. exhibit many phenotypic traits | |
| 2. |
Phagemid consist of |
| A. | plasmid vector carrying phage's cos site |
| B. | plasmid vector carrying attachment ( att) site |
| C. | plasmid vector carrying origin of replication of phage only |
| D. | plasmid vector carrying origin of replication of plasmid only |
| Answer» C. plasmid vector carrying origin of replication of phage only | |
| 3. |
Maximum size of foreign DNA that can be inserted into an insertion vector is |
| A. | 35 kb |
| B. | 18 kb |
| C. | 50 kb |
| D. | 27 kb |
| Answer» C. 50 kb | |
| 4. |
Plasmids which are maintained as multiple copy number per cell are known as |
| A. | stringent plasmids |
| B. | relaxed plasmids |
| C. | cryptic plasmids |
| D. | none of these |
| Answer» C. cryptic plasmids | |
| 5. |
EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to |
| A. | 6-7 kb |
| B. | 15-25 kb |
| C. | 40-44 kb |
| D. | 1-2 kb |
| Answer» C. 40-44 kb | |
| 6. |
Size of the DNA that can be packaged into a phage is |
| A. | 50 kb |
| B. | 35-53 kb |
| C. | 40-50 kb |
| D. | any size |
| Answer» C. 40-50 kb | |
| 7. |
Cosmid vectors are used for |
| A. | cloning small fragments of DNA |
| B. | cloning large fragments of DNA |
| C. | cloning prokaryotic DNA only |
| D. | cloning eukaryotic DNA only |
| Answer» C. cloning prokaryotic DNA only | |
| 8. |
Plasmids which are maintained as limited number of copies per cell are known as |
| A. | stringent plasmids |
| B. | relaxed plasmids |
| C. | cryptic plasmids |
| D. | all of these |
| Answer» B. relaxed plasmids | |
| 9. |
M 13 is an example of |
| A. | filamentous phage |
| B. | single stranded DNA vector |
| C. | both (a) and (b) |
| D. | plasmid |
| Answer» D. plasmid | |
| 10. |
Phagemid vectors are |
| A. | combination of plasmid and phage |
| B. | combination of phages and cosmid |
| C. | phages carrying properties of plasmids |
| D. | all of the above |
| Answer» B. combination of phages and cosmid | |
| 11. |
Cosmid vectors are |
| A. | plasmids that contain fragment of DNA including the cos site |
| B. | phages that lack cos site |
| C. | plasmids that have no selection marker |
| D. | cryptic plasmids |
| Answer» B. phages that lack cos site | |
| 12. |
P1 cloning vector is the example of |
| A. | plasmid |
| B. | cosmid |
| C. | bacteriophage |
| D. | phagemid |
| Answer» D. phagemid | |
| 13. |
ZAP vector is an example of |
| A. | phage |
| B. | phagemid |
| C. | cosmid |
| D. | plasmid |
| Answer» C. cosmid | |
| 14. |
Stuffer is |
| A. | the right arm of the vector DNA |
| B. | the left arm of the vector DNA |
| C. | central fragment of the vector DNA |
| D. | none of the above |
| Answer» D. none of the above | |
| 15. |
Cosmids lack |
| A. | genes coding for viral proteins |
| B. | origin of replication |
| C. | marker genes coding for replication |
| D. | cleavage site for the insertion of foreign DNA |
| Answer» B. origin of replication | |
| 16. |
Maximum size of foreign DNA that can be inserted into a replacement vector is |
| A. | 25-30 kb |
| B. | 18-20 kb |
| C. | 20-25 kb |
| D. | 40-50 kb |
| Answer» D. 40-50 kb | |
| 17. |
A plasmid can be considered as a suitable cloning vector if |
| A. | it can be readily isolated from the cells |
| B. | it possesses a single restriction site for one or more restriction enzymes |
| C. | insertion of foreign DNA does not alter its replication properties |
| D. | All of the above |
| Answer» E. | |
| 18. |
Inserted DNA in gt 11 can be expressed as |
| A. | -galactosidase fused protein |
| B. | free protein in the cytoplasm |
| C. | free protein that is secreted out |
| D. | all of the above |
| Answer» B. free protein in the cytoplasm | |
| 19. |
P1 cloning vector allow cloning of DNA of the length of |
| A. | 100 kbp |
| B. | 50 kbp |
| C. | 20 kbp |
| D. | 10 kbp |
| Answer» B. 50 kbp | |
| 20. |
Difference between gt 10 and gt 11 vectors is that |
| A. | gt 11 is an expression vector |
| B. | gt 10 is an expression vector |
| C. | gt 10 is a replacement vector |
| D. | gt 11 is a replacement vector |
| Answer» B. gt 10 is an expression vector | |
| 21. |
Charon 34 and Charon 35 are the examples of |
| A. | plasmid vector |
| B. | cosmid vector |
| C. | phage vector |
| D. | phagemid vector |
| Answer» D. phagemid vector | |
| 22. |
Which of the following is not true about phagemid? |
| A. | Contain functional origin of replication of the plasmid and phage |
| B. | May be propagated as a plasmid or as phage in appropriate strain |
| C. | Contain att site |
| D. | Can only be propagated as phage |
| Answer» E. | |
| 23. |
Plasmid incompatibility is |
| A. | inability of a plasmid to grow in the host |
| B. | inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. |
| C. | both (a) and (b) |
| D. | none of the above |
| Answer» C. both (a) and (b) | |
| 24. |
gt 10 and gt 11 vectors can propagate cloned fragments up to |
| A. | 6-7 kb |
| B. | 1-2 kb |
| C. | 40-44 kb |
| D. | 20-23 kb |
| Answer» B. 1-2 kb | |
| 25. |
Single stranded vectors are useful |
| A. | for sequencing of cloned DNA |
| B. | for oligonucleotide directed mutagenesis |
| C. | for probe preparation |
| D. | all of the above |
| Answer» E. | |
| 26. |
Cos site of the cosmids |
| A. | consists of 12 bases |
| B. | helps whole genome in circularization and ligation |
| C. | both (a) and (b) |
| D. | contains cleavage site |
| Answer» D. contains cleavage site | |
| 27. |
Select the wrong statement about plasmids? |
| A. | It is extrachromosomal |
| B. | It is double stranded |
| C. | Its replication depends upon host cell |
| D. | It is closed and circular DNA |
| Answer» D. It is closed and circular DNA | |
| 28. |
Charon 34 and Charon 35 can clone DNA upto |
| A. | 1-2 kb |
| B. | 6-7 kb |
| C. | 9-20 kb |
| D. | 30-35 kb |
| Answer» D. 30-35 kb | |
| 29. |
Charon vectors are different from EMBL vectors because |
| A. | they have more extensive range of restriction targets with in their polylinkers. |
| B. | physical separation of lambda arm from central fragment is required |
| C. | both (a) and (b) |
| D. | physical separation of lambda arm from central fragment is not required |
| Answer» D. physical separation of lambda arm from central fragment is not required | |