MCQOPTIONS

Explore topic-wise MCQs in Biotechnology.

This section includes 19 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.

1.	During which phase of growth of Penicillium chrysogenum maximum antibiotic production takes place
A.	during the first phase
B.	during the second phase
C.	during the third phase
D.	same in all the phases
Answer» C. during the third phase

2.	The yield of the antibiotic depends upon
A.	pH of the medium
B.	age of the inoculum
C.	composition of the medium
D.	all of these
Answer» E.

3.	Which of the following event occurs during second phase of growth of P. chrysogenum?
A.	Synthesis of penicillin is high
B.	Mycelial mass increases
C.	pH increase
D.	Both (a) and (b)
Answer» E.

4.	Inoculum preparation for the fermentation medium for penicillin takes place on
A.	wheat seeds
B.	barley seeds
C.	rice seeds
D.	all of these
Answer» C. rice seeds

5.	Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as
A.	source of nutrients
B.	carbon source
C.	antifoam agent
D.	both (a) and (c)
Answer» D. both (a) and (c)

6.	Which of the following organism(s) produce(s) tetracycline?
A.	<i>Steptomyces aureofaciens</i>
B.	<i>Steptomyces ramosus</i>
C.	<i>Nocardia sulphurea</i>
D.	All of these
Answer» E.

7.	High yield of chlorotetracyclin requires
A.	no aeration
B.	continuous aeration
C.	aeration which does not affect the yield
D.	controlled aeration
Answer» C. aeration which does not affect the yield

8.	At acidic pH, penicillin remains in
A.	aqueous phase
B.	solvent phase
C.	both (a) and (b)
D.	precipitates
Answer» C. both (a) and (b)

9.	Chlorotetracyclin is soluble in
A.	organic solvents
B.	water
C.	ether
D.	all of these
Answer» C. ether

10.	Penicillin is recovered after fermentation as
A.	potassium penicillin
B.	calcium penicillin
C.	sodium penicillin
D.	penicillin only
Answer» B. calcium penicillin

11.	pH of the fermentation medium for chlorotetracyclins is
A.	5-6
B.	6-7
C.	7-8
D.	8-9
Answer» B. 6-7

12.	Antibiotics are typically produced in fed batch reactors because
A.	antibiotic yields are generally higher when cells enter the stationary phase
B.	the precursors are often toxic to the cells
C.	antibiotic yields are generally higher when cell growth slows
D.	All of the above
Answer» E.

13.	Which of the following event occurs during third phase of growth of P chrysogenum?
A.	Concentration of antibiotic increases in the medium
B.	Autolysis of the medium starts
C.	Slight rise in pH due to liberation of ammonia
D.	All of the above
Answer» E.

14.	At normal pH, penicillin remains in
A.	aqueous phase
B.	solvent phase
C.	both (a) and (b)
D.	precipitates
Answer» B. solvent phase

15.	Which of the following precursor is added in the medium to get penicillin G?
A.	Phenyl carbamic acid
B.	Phenyl acetic acid
C.	Ammonium sulphate
D.	Ammonium chloride
Answer» C. Ammonium sulphate

16.	Which of the following changes occur during first phase of growth of Penicillium chrysogenum?
A.	Growth of mycelia occurs
B.	Ammonia is liberated in the medium
C.	Lactic acid present in the corn steep liquor is utilized at the maximum rate
D.	All of the above
Answer» E.

17.	Penicillin is produced by
A.	aerobic fermentation
B.	anaerobic fermentation
C.	aerobic fermentation followed by anaerobic fermentation
D.	anaerobic fermentation followed by aerobic fermentation
Answer» B. anaerobic fermentation

18.	Media composition for the production of streptomycin is
A.	soybean meal, glucose, peptone, malt extract, sodium chloride
B.	soybean meal, glucose, peptone, malt extract, ammonium chloride
C.	soybean meal, glucose, peptone, malt extract, calcium carbonate
D.	soybean meal, glucose, peptone, malt extract, ammonium sulphate
Answer» B. soybean meal, glucose, peptone, malt extract, ammonium chloride

19.	Streptomycin is produced by
A.	<i>S. griseus</i>
B.	<i>S. griseoflavus</i>
C.	<i>S. aerofaciens</i>
D.	<i>S. ramosus</i>
Answer» B. <i>S. griseoflavus</i>