34 + Mcqs in Animal Cell Culture and Regulation Page 1 McqOptions

1.	Excess CO suppress cell growth and productivity by
A.	inhibiting respiration
B.	altering intracellular pH by diffusing across cell membrane
C.	both (a) and (b)
D.	altering pH of the medium
Answer» D. altering pH of the medium

Discussion

2.	How does CO help in the cell metabolism during cell culture?
A.	It participates in the de novo synthesis of purines and pyrimidines
B.	Helps in the cells respiration
C.	For monitoring pH of the culture
D.	All of the above
Answer» B. Helps in the cells respiration

Discussion

3.	Range of osmolarity tolerated/accepted in mOsm/Kg of HO by mammalian cells is
A.	150-300
B.	280-360
C.	300-325
D.	360-400
Answer» C. 300-325

Discussion

4.	What is the concentration of CO required for culturing animal cells?
A.	2-5%
B.	1-10%
C.	10-15%
D.	15-20%
Answer» C. 10-15%

Discussion

5.	Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
A.	established cell lines
B.	primary cell lines
C.	secondary cell lines
D.	propagated cell lines
Answer» B. primary cell lines

Discussion

6.	In animal cell culture, particularly mammalian cell culture, transformation means
A.	uptake of new genetic material
B.	phenotypic modifications of cells in culture
C.	both (a) and (b)
D.	release of genetic information
Answer» C. both (a) and (b)

Discussion

7.	During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
A.	Ethyl alcohol is being produced in excess
B.	The cells have too much oxygen
C.	Glycolysis is being inhibited
D.	The cells do not have enough oxygen
Answer» E.

Discussion

8.	Range of optimum glucose concentration present in the culture media is
A.	5.5 - 55 mmol/litre
B.	55 - 75 mmol/litre
C.	75-105 mmol/litre
D.	105-150 mmol/litre
Answer» B. 55 - 75 mmol/litre

Discussion

9.	Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
A.	Down's syndrome
B.	Turner's syndrome
C.	Klinefelter's syndrome
D.	all of these
Answer» E.

Discussion

10.	An established cell line can be called where it has been subcultured at least
A.	70 times at an interval of 3 days between subcultures
B.	40 times at an interval of 3 days between subcultures
C.	70 times at an interval of 1 day between subcultures
D.	50 times at an interval of 3 days between subcultures
Answer» B. 40 times at an interval of 3 days between subcultures

Discussion

11.	Which of the following is not a source of energy in active muscle cells?
A.	Creatine phosphate
B.	ATP
C.	Lactic acid
D.	Glucose
Answer» D. Glucose

Discussion

12.	Which of the following is not the explantation technique?
A.	Slide culture
B.	Carrel flask culture
C.	Roller test tube culture
D.	Adherent primary culture
Answer» E.

Discussion

13.	Cells which have undergone transformation frequently become
A.	anchorage independent
B.	anchorage dependent
C.	stable
D.	unstable
Answer» B. anchorage dependent

Discussion

14.	pH of culture medium is initially controlled by
A.	presence of CO
B.	<sub>2</sub>
C.	presence of bicarbonate buffer
D.	addition of bases
E.	none of these
Answer» C. presence of bicarbonate buffer

Discussion

15.	What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?
A.	They act as growth promoters
B.	They act as growth inhibitors
C.	Have no effect on cells
D.	Lactate helps in the growth while ammonium inhibits the growth
Answer» E.

Discussion

16.	What are the main constituents of culture for animal cell growth?
A.	Glucose and Glutamine
B.	Growth factors
C.	Cytokines
D.	All of these
Answer» B. Growth factors

Discussion

17.	To prevent the accumulation of lactate
A.	low glutamine concentration is required
B.	high glutamine concentration is required
C.	low glucose concentration is required
D.	high glucose concentration is required
Answer» D. high glucose concentration is required

Discussion

18.	According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
A.	6 amino acids
B.	8 amino acids
C.	10 amino acids
D.	12 amino acids
Answer» E.

Discussion

19.	What is the concentration of CO2 required for culturing animal cells?
A.	2-5%
B.	1-10%
C.	10-15%
D.	15-20%
Answer» C. 10-15%

Discussion

20.	When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
A.	incomplete glutamine oxidation
B.	increase in specific lactate production from glucose
C.	both (a) and (b)
D.	accumulation of ammonia
Answer» D. accumulation of ammonia

Discussion

21.	The human fibroplast is a classical example of
A.	stable primary cell lines
B.	established cell lines
C.	cell transformations
D.	none of these
Answer» B. established cell lines

Discussion

22.	Range of optimum glutamine concentration present in the culture media is
A.	1-2 mmol/litre
B.	2-7 mmol/litre
C.	7-15 mmol/litre
D.	15 - 20 mmol/litre
Answer» C. 7-15 mmol/litre

Discussion

23.	For culturing, plasma from the adult chicken is preferred to mammalian plasma because
A.	it forms a clear and solid coagulum even after dilution
B.	it is too opaque
C.	it doesn't produce solid clots
D.	it forms a semi solid coagulum
Answer» B. it is too opaque

Discussion

24.	Disaggregating of cells can be achieved by
A.	physical disruption
B.	enzymatic digestion
C.	treating with chelating agents
D.	all of the above
Answer» E.

Discussion

25.	Toxicity due to accumulation of ammonia can be overcome
A.	by substituting glutamine by glutamate
B.	by controlled addition of glutamine at low level
C.	by removal of ammonia or ammonium from culture medium
D.	all of the above
Answer» E.

Discussion

26.	The major problem associated with the isolation of free cells and cell aggregates from organs is that of
A.	releasing the cells from their supporting matrix
B.	inhibiting the cells from their supporting matrix
C.	disintegrating the cells from their supporting matrix
D.	none of the above
Answer» B. inhibiting the cells from their supporting matrix

Discussion

27.	Accumulation of lactate leads to
A.	increase in pH
B.	no change in pH
C.	reduction in the pH of culture hence loss of cell viability
D.	no loss of cell viability
Answer» D. no loss of cell viability

Discussion

28.	Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
A.	150-300
B.	280-360
C.	300-325
D.	360-400
Answer» C. 300-325

Discussion

29.	Excess CO2 suppress cell growth and productivity by
A.	inhibiting respiration
B.	altering intracellular pH by diffusing across cell membrane
C.	both (a) and (b)
D.	altering pH of the medium
Answer» D. altering pH of the medium

Discussion

30.	Which of the following is the technique used for the embryo culture?
A.	Organ cultures on plasma clots
B.	Organ cultures on agar
C.	Whole embryo cultures
D.	All of these
Answer» E.

Discussion

31.	How does CO2 help in the cell metabolism during cell culture?
A.	It participates in the de novo synthesis of purines and pyrimidines
B.	Helps in the cells respiration
C.	For monitoring pH of the culture
D.	All of the above
Answer» B. Helps in the cells respiration

Discussion

32.	The technique of organ culture may be divided on the basis of employing
A.	solid medium
B.	liquid medium
C.	both (a) and (b)
D.	semi-solid medium
Answer» D. semi-solid medium

Discussion

33.	At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
A.	oxidative pathway
B.	anaerobic pathway
C.	both (a) and (b)
D.	none of these
Answer» B. anaerobic pathway

Discussion

34.	Higher dissolved oxygen concentration in the culture media are toxic and lead to
A.	DNA degradation
B.	lipid peroxidation
C.	metabolism of nutrients in culture media at a rate greater than that required for consumption
D.	all of the above
Answer» E.

Discussion

Explore topic-wise MCQs in Biotechnology.

Excess CO suppress cell growth and productivity by

How does CO help in the cell metabolism during cell culture?

Range of osmolarity tolerated/accepted in mOsm/Kg of HO by mammalian cells is

What is the concentration of CO required for culturing animal cells?

Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

In animal cell culture, particularly mammalian cell culture, transformation means

During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

Range of optimum glucose concentration present in the culture media is

Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

An established cell line can be called where it has been subcultured at least

Which of the following is not a source of energy in active muscle cells?

Which of the following is not the explantation technique?

Cells which have undergone transformation frequently become

pH of culture medium is initially controlled by

What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

What are the main constituents of culture for animal cell growth?

To prevent the accumulation of lactate

According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

What is the concentration of CO2 required for culturing animal cells?

When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

The human fibroplast is a classical example of

Range of optimum glutamine concentration present in the culture media is

For culturing, plasma from the adult chicken is preferred to mammalian plasma because

Disaggregating of cells can be achieved by

Toxicity due to accumulation of ammonia can be overcome

The major problem associated with the isolation of free cells and cell aggregates from organs is that of

Accumulation of lactate leads to

Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

Excess CO2 suppress cell growth and productivity by

Which of the following is the technique used for the embryo culture?

How does CO2 help in the cell metabolism during cell culture?

The technique of organ culture may be divided on the basis of employing

At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

Higher dissolved oxygen concentration in the culture media are toxic and lead to