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This section includes 38 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The nucleous of mature unfertilized ovum may be removed by |
| A. | irradiation |
| B. | surgery |
| C. | both (a) and (b) |
| D. | neutralization and homogenization |
| Answer» D. neutralization and homogenization | |
| 2. |
Karyoplast is |
| A. | cells devoid of cell wall |
| B. | nuclei |
| C. | nuclei with only some residual plasma membrane |
| D. | cell with nucleus |
| Answer» D. cell with nucleus | |
| 3. |
The success of embryo transplantation depends upon the ability to obtain an increased number of embryos from animals of superior genetic merit, which can be achieved by the use of |
| A. | exogenous gonadatrophic hormones |
| B. | endogenous gonadatrophic hormones |
| C. | both (a) and (b) |
| D. | FSH (Follicle Stimulating Hormone) |
| Answer» B. endogenous gonadatrophic hormones | |
| 4. |
Multiple ovulation and embryo transfer (MOET) |
| A. | can increase the rate of progress in dairy cattle |
| B. | allows progeny testing of males |
| C. | allows progeny testing of females |
| D. | all of the above |
| Answer» E. | |
| 5. |
The technique, mainly used for the diagnosing birth defects in the fetus by means of needle, is called |
| A. | amniocentesis |
| B. | ectogensis |
| C. | transplantation |
| D. | all of the above |
| Answer» B. ectogensis | |
| 6. |
DNA is microinfected into the fertilized egg |
| A. | after the fusion of male and female nuclei |
| B. | before the fusion of male and female nuclei |
| C. | at the time of fusion of male and female nuclei |
| D. | any time, it can be infected |
| Answer» C. at the time of fusion of male and female nuclei | |
| 7. |
In which year, Dolly the first mammalian clone was born? |
| A. | 1995 |
| B. | 1997 |
| C. | 1996 |
| D. | 1998 |
| Answer» D. 1998 | |
| 8. |
Embryo transfer (ET) refers to the technique by which fertilized ova are collected from the reproductive tract of a genetically |
| A. | superior female and transferred to the inferior |
| B. | inferior female and transferred to the superior |
| C. | balanced female |
| D. | none of the above |
| Answer» B. inferior female and transferred to the superior | |
| 9. |
The number of follicles at any particular stage is governed by |
| A. | the rate of entry of dormant follicles |
| B. | the rate of growth of follicles |
| C. | the rate of loss of follicles |
| D. | all of the above |
| Answer» E. | |
| 10. |
Production of transgenic animals require transfections of |
| A. | eggs or embryos |
| B. | stem cells |
| C. | red blood cells |
| D. | all of these |
| Answer» B. stem cells | |
| 11. |
Which of the following gene have been introduced into the transgenic fish? |
| A. | Human or rat gene for growth hormone |
| B. | Chicken gene for delta crystalline protein |
| C. | <i>E. coli</i> |
| D. | gene for -galactosidase |
| E. | All of the above |
| Answer» E. All of the above | |
| 12. |
Enucleation of the cells can be achieved by treating the cells with |
| A. | polyethylene glycol |
| B. | cytochalasin B |
| C. | both (a) and (b) |
| D. | alcohol |
| Answer» C. both (a) and (b) | |
| 13. |
When the cells are enucleated and centrifuged, nuclei |
| A. | detach from cell and settle at the bottom |
| B. | detach from cell and remain in the supernatant |
| C. | nuclei remain attached to the cell |
| D. | none of the above |
| Answer» B. detach from cell and remain in the supernatant | |
| 14. |
Which of the following statements best describes a clone? |
| A. | An artificial life form |
| B. | An offspring where all of the genetic material in every cell is identical to that of both parents |
| C. | An offspring where all of the genetic material in every cell is identical to that of one of its parents |
| D. | A type of sheep |
| Answer» D. A type of sheep | |
| 15. |
Which of these carries the thousands of genes needed to allow animal and plant cells to pass on inherited characteristics? |
| A. | Chromosomes |
| B. | Chromatogram |
| C. | Chromatography |
| D. | Chromium |
| Answer» B. Chromatogram | |
| 16. |
In transgenic fish, the genes are introduced by |
| A. | microinjection in fish |
| B. | viruses |
| C. | transfer of whole nuclei |
| D. | all of these |
| Answer» B. viruses | |
| 17. |
Animal pharming can be defined as |
| A. | growing animals for farming |
| B. | programming animals to produce novel products |
| C. | generating transgenic animals for farming |
| D. | none of the above |
| Answer» C. generating transgenic animals for farming | |
| 18. |
When a fertilized egg cell develops into an embryo, the entropy of the living system |
| A. | decreases |
| B. | increases |
| C. | remains constant |
| D. | none of these |
| Answer» B. increases | |
| 19. |
For the transfer of whole individual chromosomes, they are isolated from the cells at |
| A. | prophase |
| B. | metaphase |
| C. | telophase |
| D. | anaphase |
| Answer» C. telophase | |
| 20. |
Fusion of karyoplast with the enucleated cell is achieved in presence of |
| A. | cytochalasin B |
| B. | polyethylene glycol |
| C. | both (a) and (b) |
| D. | alcohol |
| Answer» C. both (a) and (b) | |
| 21. |
Transfection refers to which of the following? |
| A. | Synthesis of mRNA from DNA template |
| B. | Synthesis of protein based on mRNA sequence |
| C. | Introduction of foreign gene in to a cell |
| D. | The process by which a cell become malignant |
| Answer» D. The process by which a cell become malignant | |
| 22. |
Chromosomes may be isolated from metaphase cells by |
| A. | hypertonic lysis |
| B. | hypotonic lysis |
| C. | either (a) or (b) |
| D. | isotonic lysis |
| Answer» C. either (a) or (b) | |
| 23. |
Superovulation is primarily the result of |
| A. | decreased atresia in medium and large follicles (>1.7 mm) |
| B. | increased atresia in medium and large follicles (>1.7 mm) |
| C. | increased atresia in the large follicles (<1.7 mm) |
| D. | decreased atresia in small follicles (<1.7 mm) |
| Answer» B. increased atresia in medium and large follicles (>1.7 mm) | |
| 24. |
Genetic change can occur only in |
| A. | one way |
| B. | two ways |
| C. | three ways |
| D. | four ways |
| Answer» E. | |
| 25. |
Superovulation is an |
| A. | increased ovulatory response by external hormonal therapy |
| B. | decreased ovulatory response by external hormonal therapy |
| C. | decreased ovulatory response by internal hormonal therapy |
| D. | increased ovulatory response by internal hormonal therapy |
| Answer» B. decreased ovulatory response by external hormonal therapy | |
| 26. |
Which of the following best describes artificial insemination? |
| A. | Transplanting an embryo into the uterus |
| B. | Fertilization of an egg in a test tube |
| C. | Selectively breeding healthy animals |
| D. | Taking the sperm and placing it directly |
| Answer» E. | |
| 27. |
Which part of a cell carries the information that is passed from one generation to the next? |
| A. | Cell |
| B. | Nucleus |
| C. | Cytoplasm |
| D. | Chloroplast |
| Answer» C. Cytoplasm | |
| 28. |
Transgenic goats produce a variant of human tissue type plasminogen activator protein in |
| A. | blood |
| B. | urine |
| C. | milk |
| D. | muscles |
| Answer» D. muscles | |
| 29. |
For the rapid genetic progress, embryo transfer (ET) |
| A. | will be as potent a tool as AI (artificial insemination) |
| B. | will not be as potent a tool as AI (artificial insemination) |
| C. | ET can't be used at all |
| D. | will be a superior tool than AI |
| Answer» C. ET can't be used at all | |
| 30. |
Cloning is a method by which numbers of genetically identical organisms are derived from a single organism by |
| A. | vegetative propagation |
| B. | vegetative initiation |
| C. | vegetative termination |
| D. | none of these |
| Answer» B. vegetative initiation | |
| 31. |
Which of the following is/are the method of transfection for making transgenic animals? |
| A. | Transfer of whole nuclei |
| B. | Transfer of whole individual chromosomes or fragment |
| C. | Transfer of DNA |
| D. | All of the above |
| Answer» E. | |
| 32. |
DNA microinjection into the egg has been used to produce which of the following transgenic animals? |
| A. | Mice |
| B. | Chicken |
| C. | Pigs |
| D. | All of these |
| Answer» E. | |
| 33. |
Which of these established cell lines originate from a mouse embryo? |
| A. | 3T3 |
| B. | BHK |
| C. | HeLa |
| D. | BTK |
| Answer» B. BHK | |
| 34. |
Some types of variation are due to changes in the genetic material. What is this type of change called? |
| A. | Fertilisation |
| B. | Mutation |
| C. | Radiation |
| D. | Sterilisation |
| Answer» C. Radiation | |
| 35. |
Which of the following is the process of choosing parent organisms for the characteristics that is wanted in their offspring? |
| A. | Active selection |
| B. | Reproductive selection |
| C. | Selective breeding |
| D. | Breeding selection |
| Answer» D. Breeding selection | |
| 36. |
Which protein has been produced generating a transgenic sheep that is used for replacement therapy for individuals at risk from emphysema? |
| A. | Plasminogen activator (tPA) |
| B. | -anti trypsin (AAT) |
| C. | Casein |
| D. | Amyloid precursor proteins |
| Answer» C. Casein | |
| 37. |
Which of the following is the word used to describe what happens when the nucleus of a sperm joins with the nucleus of an egg cell? |
| A. | Sex |
| B. | Fertilisation |
| C. | Intercourse |
| D. | Reproduction |
| Answer» C. Intercourse | |
| 38. |
DNA into fish is injected into |
| A. | pronuclei |
| B. | cytoplasm |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» C. both (a) and (b) | |