MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following statements is true about two-dimensional electrophoresis? |
| A. | Separates proteins of identical molecular weight, same pI but different charge |
| B. | Separates proteins of different molecular weight and different pI |
| C. | Separates proteins of identical molecular weight that differ in pI |
| D. | Isoelectric focusing is also termed as two-dimensional electrophoresis |
| Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis | |
| 2. |
Which of the following statements is true about affinity chromatography? |
| A. | During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first |
| B. | During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last |
| C. | During the separation of a mixture of proteins, the protein which binds to ligand is eluted first |
| D. | Unwanted proteins are eluted by ligand solution |
| Answer» B. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last | |
| 3. |
The pattern on paper in chromatography is called __________ |
| A. | Chroming |
| B. | Chroma |
| C. | Chromatograph |
| D. | Chromatogram |
| Answer» E. | |
| 4. |
Mobile phase can be ___________ |
| A. | Only solid |
| B. | Only gas |
| C. | Solid or liquid |
| D. | Liquid or gas |
| Answer» E. | |
| 5. |
The salt which produces salting out effect during extraction of proteins is? |
| A. | NH4 SO4 |
| B. | (NH4)2 SO4 |
| C. | (NH4)3 SO4 |
| D. | NaCl |
| Answer» C. (NH4)3 SO4 | |
| 6. |
WHICH_OF_THE_FOLLOWING_STATEMENTS_IS_TRUE_ABOUT_TWO-DIMENSIONAL_ELECTROPHORESIS??$ |
| A. | Separates proteins of identical molecular weight, same pI but different charge |
| B. | Separates proteins of different molecular weight and different pI |
| C. | Separates proteins of identical molecular weight that differ in pI |
| D. | Isoelectric focusing is also termed as two-dimensional electrophoresis |
| Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis | |
| 7. |
Which_of_the_following_statements_is_false?$ |
| A. | The term activity refers to the total units of enzyme in a solution |
| B. | The specific activity is a measure of enzyme purity |
| C. | Specific activity increases during purification of an enzyme and becomes maximal and constant when the enzyme is pure |
| D. | Specific activity decreases during purification of an enzyme and becomes maximal and constant when the enzyme is pure |
| Answer» E. | |
| 8. |
Which of the following statements is true about SDS polyacrylamide chromatography? |
| A. | SDS polyacrylamide gel electrophoresis separates proteins on the basis of size |
| B. | SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge |
| C. | SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids |
| D. | SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per one amino acid |
| Answer» C. SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids | |
| 9. |
Which of the following statements is true about ion-exchange chromatography? |
| A. | It separates proteins according to their size |
| B. | The column matrix with bound anionic groups is called cationic exchanger |
| C. | The column matrix with bound anionic groups is called anionic exchanger |
| D. | The column matrix with bound cationic groups is called cationic exchanger |
| Answer» C. The column matrix with bound anionic groups is called anionic exchanger | |
| 10. |
Which of the following statements is true about size-exclusion chromatography? |
| A. | During the separation of a mixture of proteins, protein with smallest molecular weight is eluted first |
| B. | During the separation of a mixture of proteins, protein with largest molecular weight is eluted first |
| C. | During the separation of a mixture of proteins, protein with largest molecular weight is eluted last |
| D. | During the separation of a mixture of proteins, protein with largest molecular weight flow around the beads |
| Answer» C. During the separation of a mixture of proteins, protein with largest molecular weight is eluted last | |
| 11. |
Which of the following statements about column chromatography is correct? |
| A. | Resolution increases as the length of the column increases |
| B. | Mobile phase is a porous solid material with appropriate chemical properties held in the column |
| C. | Stationary phase is a buffered solution that percolates through mobile phase |
| D. | Large proteins emerge from the column sooner than small ones |
| Answer» B. Mobile phase is a porous solid material with appropriate chemical properties held in the column | |
| 12. |
The pattern on paper in chromatography is called |
| A. | Chroming |
| B. | Chroma |
| C. | Chromatograph |
| D. | Chromatogram |
| Answer» E. | |
| 13. |
Mobile phase can be |
| A. | Only solid |
| B. | Only gas |
| C. | Solid or liquid |
| D. | Liquid or gas |
| Answer» E. | |
| 14. |
The salt which produces salting out effect during extraction of proteins is |
| A. | NH<sub>4</sub> SO<sub>4</sub> |
| B. | (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub> |
| C. | (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub> |
| D. | NaCl |
| Answer» C. (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub> | |