MCQOPTIONS
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MCQOPTIONS
This section includes 96 Mcqs, each offering curated multiple-choice questions to sharpen your Cpp Interview knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Choose the incorrect statement with respect to rolling circle replication. |
| A. | It is for a single replication fork and yields a concatemeric molecule |
| B. | Staggered cleavage of DNA takes place at the cos sites |
| C. | The cleavage generates 10 nucleotide long overhangs |
| D. | Without the cos sites, the packaging won’t be possible |
| Answer» D. Without the cos sites, the packaging won’t be possible | |
| 52. |
What is the function of red and gam gene products? |
| A. | It promotes the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P1 |
| B. | It inhibits the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P2 |
| C. | It inhibits the growth of the phage in the E. coli cells which are lytic for bacteriophage P1 |
| D. | It activates the growth of the phage in the E. coli cells which are lytic for bacteriophage P2 |
| Answer» C. It inhibits the growth of the phage in the E. coli cells which are lytic for bacteriophage P1 | |
| 53. |
Bacteriophage Mu is also a phage, choose the correct statement for it. |
| A. | It is an example of temperate phage |
| B. | It is packaged into heads containing 50 kbp of DNA |
| C. | When the phage replicates it goes transposition only a few times |
| D. | Mu can’t be used for cloning in vivo |
| Answer» B. It is packaged into heads containing 50 kbp of DNA | |
| 54. |
Choose the correct statement for Sanger’s method of sequencing. |
| A. | They are also called as chain termination methods |
| B. | Use of M13 vector is made for getting double stranded DNA |
| C. | Use of dideoxynucleotide phosphate (ddNTP) is made |
| D. | The use of M13 vectors is more preferred these days |
| Answer» B. Use of M13 vector is made for getting double stranded DNA | |
| 55. |
Circularization must take place in the bacterial cells before the replication starts. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't Say |
| Answer» B. False | |
| 56. |
Choose the correct statement for BAC vector system. |
| A. | BAC vector system stands for bacteria and chromosome |
| B. | It usually accepts insert of size approximately 1000kbp |
| C. | The repE and oriS sequences are required for controlling the copy number and par A-C sequences are required for replication |
| D. | A selectable marker is there for chloramphenicol resistance |
| Answer» E. | |
| 57. |
Which of the following is used for blocking the phage-encoded recombination? |
| A. | Mutation in D gene |
| B. | Mutation in E gene |
| C. | Red mutation |
| D. | Mutation in S gene |
| Answer» D. Mutation in S gene | |
| 58. |
Choose incorrect statement for the substitution vector EMBL4. |
| A. | It is having two single multiple cloning sites |
| B. | The region to be removed is on one side of the multiple cloning sites |
| C. | If a digestion within the multiple cloning sites, it generates central portion and right and left arms |
| D. | The right and left portions are called as arms |
| Answer» C. If a digestion within the multiple cloning sites, it generates central portion and right and left arms | |
| 59. |
The main advantage of phagemid is that it can be used for the generation of single or double stranded products without recloning. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 60. |
What is the function of the pac site in the bacteriophage PI? |
| A. | It is responsible for the initiation of packaging |
| B. | It is responsible for the termination of packaging |
| C. | It is responsible for the initiation of replication |
| D. | It is responsible for the termination of replication |
| Answer» B. It is responsible for the termination of packaging | |
| 61. |
Choose the incorrect statement for lysogenic mode. |
| A. | PRE forms cI gene product which is responsible for activation of promoters |
| B. | The cII protein is responsible for interfering with cro and Q proteins and they are required for lytic life cycle |
| C. | The cII protein activates the promoter PINT |
| D. | The Int protein with host protein is responsible for site specific recombination |
| Answer» B. The cII protein is responsible for interfering with cro and Q proteins and they are required for lytic life cycle | |
| 62. |
Choose the correct statement for the infection process of M13. |
| A. | The infectious particle is double stranded |
| B. | It is contained in a protein coat which is made up of products of gene III or gene VIII |
| C. | The phage infects only female cells |
| D. | The phage enters the bacterial cells by the filament which is meant for movement |
| Answer» C. The phage infects only female cells | |
| 63. |
Which of the following is a restriction for cloning in bacteriophage Mu? |
| A. | It is limited to handling only small number of phage particles |
| B. | It relies on whether the DNA is flanked by two mini Mu genes or not |
| C. | Recombination between the elements in the second strain is not a restriction |
| D. | Fragments of any genomic DNA can be cloned irrespective of the fact whether or not the phage can replicate in it or not |
| Answer» C. Recombination between the elements in the second strain is not a restriction | |
| 64. |
Choose the correct statement for the cI gene. |
| A. | It represses only cII gene |
| B. | It activates cII gene and represses cIII gene |
| C. | It represses both cII and cIII gene |
| D. | It activates both cII and cIII gene |
| Answer» D. It activates both cII and cIII gene | |
| 65. |
If a single system is used for packaging, there are increased chances of endogenous material being packed. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 66. |
Choose the incorrect statement for the replication process. |
| A. | The initiator site is nicked and replication of one strand is started |
| B. | Replication takes place in both the directions |
| C. | Replication continues through the bluescript |
| D. | It is stopped at the terminator and then again a nick is made |
| Answer» C. Replication continues through the bluescript | |
| 67. |
How many origins of replication are there once the cyclization is carried out by loxP sites? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 68. |
Choose the correct statement for the lambda ZAP vector. |
| A. | It is not based on bacteriophage lambda |
| B. | It contains a region that can be excised in vivo |
| C. | The excision leads to the formation of bluescript plasmids and it contains an initiator region only |
| D. | The multiple cloning site is not flanked by the initiator and the terminator region |
| Answer» C. The excision leads to the formation of bluescript plasmids and it contains an initiator region only | |
| 69. |
Choose the incorrect statement with respect to the collection of single stranded molecules. |
| A. | An infected culture is set up and after a while the cells are removed by centrifugation |
| B. | The phage is in the supernatant and is precipitated by addition of glycol and sodium chloride |
| C. | The phage pellet is resuspended and phenol is used for removal of protein |
| D. | The single stranded molecules sediment at the bottom |
| Answer» E. | |
| 70. |
Choose the incorrect statement for ‘Mini Mu’? |
| A. | It is produced by cloning of bacteriophage Mu in vivo |
| B. | It contains reduced phage genome |
| C. | It doesn’t contain replication of origin of plasmid |
| D. | A selectable marker is added |
| Answer» D. A selectable marker is added | |
| 71. |
Which of the following is not a consequence of deletion in the b region? |
| A. | Excision of the prophage on induction is prevented |
| B. | It reduces the amount of endogenous phage DNA in the packaging mix |
| C. | It reduces the amount of exogenous phage DNA in the packaging mix |
| D. | It reduces the level of background non-recombinant phage |
| Answer» D. It reduces the level of background non-recombinant phage | |
| 72. |
The bacteriophage M13 vectors belong to a group of phages called as __________ |
| A. | skinny or filamentous phage |
| B. | M phage group |
| C. | single stranded phage |
| D. | double stranded phage |
| Answer» B. M phage group | |
| 73. |
The low copy number of F plasmid is essential for the stability of these plasmids. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 74. |
Choose the correct statement for the infectious particle of lambda phage. |
| A. | It is single stranded genome |
| B. | It is circular double stranded genome |
| C. | The ends are blunt with cos (cohesive) sequences |
| D. | The ends are created by cleavage at cos sites during phage packaging |
| Answer» E. | |
| 75. |
What is the function of the N gene? |
| A. | It promotes the function of terminators |
| B. | It overrides the function of terminators |
| C. | It promotes the production of the cro gene |
| D. | It is responsible for making the infectious particle linear |
| Answer» C. It promotes the production of the cro gene | |
| 76. |
There are some phages which don’t preferentially transduce some special regions of the phage genome. These phages are known as __________ |
| A. | transducing phages |
| B. | specialized transducing phages |
| C. | generalized transducing phages |
| D. | transforming phages |
| Answer» D. transforming phages | |
| 77. |
What is the function of loxP sites? |
| A. | It is responsible for linearization of DNA in the host bacterium |
| B. | It is responsible for circularization of DNA in the host bacterium |
| C. | It is responsible for conversion of single stranded DNA into double stranded DNA |
| D. | It is responsible for conversion of double stranded DNA into single stranded DNA |
| Answer» C. It is responsible for conversion of single stranded DNA into double stranded DNA | |
| 78. |
Some changes are encountered in the infectious particle as it infects the cell. Choose the incorrect statement. |
| A. | The infectious particle circularizes upon causing infection by annealing at cos sites |
| B. | There are two promoters present, PR and PL |
| C. | Upon infection only PR is activated |
| D. | Promoters give rise to immediate early transcripts |
| Answer» D. Promoters give rise to immediate early transcripts | |
| 79. |
Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 80. |
Gene V blocks the production of which gene? |
| A. | Gene III |
| B. | Gene II |
| C. | Gene VIII |
| D. | Gene IV |
| Answer» C. Gene VIII | |
| 81. |
Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 82. |
Phages which are designated as spi-are __________ |
| A. | red+ gam+ |
| B. | red+ gam- |
| C. | red- gam+ |
| D. | red- gam- |
| Answer» E. | |
| 83. |
Choose the correct statement with respect to lysogen and prophage. |
| A. | The integrated phage is called as lysogen |
| B. | The infected bacterial cell is called a prophage |
| C. | If there is only growth of few lysogens, the E. coli lawn would be turbid |
| D. | If lysogens won’t be formed, the plaques won’t be clear |
| Answer» D. If lysogens won’t be formed, the plaques won’t be clear | |
| 84. |
It is very important to study lambda biology as lambda phages are used for cloning purposes. Which of the statement is true for lambda phage? |
| A. | It is an example of temperate phage |
| B. | The fate of the phage is decided before it infects the cell |
| C. | The lysis fate is that where the phage inserts its genome into the bacterial genome and the replication goes on |
| D. | The lysogenic fate is that where the phage infects the cell and lysis is carried out |
| Answer» B. The fate of the phage is decided before it infects the cell | |
| 85. |
The presence of insert leads to inactivation of which gene? |
| A. | cII |
| B. | cI |
| C. | cIII |
| D. | both cII and cIII |
| Answer» C. cIII | |
| 86. |
Choose the correct statement for the synthesis of RNA probe? |
| A. | Double stranded DNA is only used for probe synthesis |
| B. | Single stranded DNA can be used for probe synthesis |
| C. | Probes are prepared for RNA transcripts which are specific for only particular strand |
| D. | Probes are prepared for RNA transcripts which are specific for both the strands |
| Answer» C. Probes are prepared for RNA transcripts which are specific for only particular strand | |
| 87. |
Which of the following is not included as a step for cloning mini Mu in vivo? |
| A. | A culture of target strain, whose DNA we want to clone is infected with mini Mu |
| B. | The phage is inserted at multiple sites in the genome |
| C. | Some of the packaged strain is flanked by two mini Mu genes |
| D. | The packaged phage is introduced into a second strain and Mu would replicate as a phage |
| Answer» E. | |
| 88. |
Physical separation is also used at times. Choose the correct statement with respect to this method. |
| A. | Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient |
| B. | In sugar density gradient, the molecules are separated on the basis of density |
| C. | The central portion is recovered from the gel or the gradient |
| D. | Gel is more efficient than the sugar density gradient |
| Answer» B. In sugar density gradient, the molecules are separated on the basis of density | |
| 89. |
At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process. |
| A. | It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there |
| B. | In this method, the central portion is cut with another enzyme after being separated from the arms |
| C. | The arms are also cut with this second enzyme |
| D. | It is not necessary to remove the fragments generated |
| Answer» D. It is not necessary to remove the fragments generated | |
| 90. |
Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________ |
| A. | A gene |
| B. | D gene |
| C. | E and D gene |
| D. | D and A gene |
| Answer» D. D and A gene | |
| 91. |
Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it. |
| A. | Packaging in phage coat is a more efficient process than the normal transformation process |
| B. | The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration |
| C. | Lysate constitutes of lambda proteins needed for phage assembly |
| D. | The amount of non-recombinant phage in the background is less |
| Answer» E. | |
| 92. |
Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it. |
| A. | Separate strains with chain-termination mutations in different genes for coat components are used |
| B. | Temperature sensitive cI repressor is used |
| C. | Due to heat shock packaging proteins are induced |
| D. | Packaging is carried out in each strain |
| Answer» E. | |
| 93. |
Size of a 0 |
| A. | |
| B. | Size of a 3 |
| Answer» B. Size of a 3 | |
| 94. |
500 |
| A. | 600 |
| B. | 700 |
| C. | Error |
| Answer» B. 700 | |
| 95. |
How many vector container properties are there in c++? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» B. 2 | |
| 96. |
In which type of storage location are the vector members stored? |
| A. | Contiguous storage locations |
| B. | Non-contiguous storage locations |
| C. | Contiguous & Non-contiguous storage locations |
| D. | None of the mentioned |
| Answer» C. Contiguous & Non-contiguous storage locations | |