MCQOPTIONS
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MCQOPTIONS
This section includes 96 Mcqs, each offering curated multiple-choice questions to sharpen your Cpp Interview knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which is optional in the declaration of vector? |
| A. | Type |
| B. | Name |
| C. | Vector |
| D. | Number_of_elements |
| Answer» E. | |
| 2. |
Pick out the correct statement about vector. |
| A. | vector<int> values (5) |
| B. | vector values (5) |
| C. | vector<int> (5) |
| D. | vector<5> |
| Answer» B. vector values (5) | |
| 3. |
What do vectors represent? |
| A. | Static arrays |
| B. | Dynamic arrays |
| C. | Stack |
| D. | Queue |
| Answer» C. Stack | |
| 4. |
The fragment inserted in the place of the central portion of the genome is known as __________ |
| A. | insertion fragment |
| B. | substitution fragment |
| C. | stuffer fragment |
| D. | displacement fragment |
| Answer» D. displacement fragment | |
| 5. |
Choose the incorrect statement for the central portion of the lambda genome. |
| A. | The central portion is not required for lytic growth |
| B. | The central portion includes repressor gene |
| C. | The repressor gene is not required for lysogenic growth |
| D. | It is approximately 10kbp long |
| Answer» E. | |
| 6. |
Insertion vectors are a type of vectors. Choose the correct statement for this type of vector. |
| A. | These are complex vectors |
| B. | DNA is inserted through many restriction sites |
| C. | There is an upper limit on the size of the DNA to be packaged |
| D. | Lambda gt09 is an example of this type of vector |
| Answer» D. Lambda gt09 is an example of this type of vector | |
| 7. |
The initiator is recognized by which gene? |
| A. | Gene I |
| B. | Gene I and II |
| C. | Only gene II |
| D. | Gene III |
| Answer» D. Gene III | |
| 8. |
The phase generating additional circular DNA molecules in the first phase does it by __________ |
| A. | bidirectional theta mode |
| B. | rolling circle mode |
| C. | separation of the two strands |
| D. | copying only one strand |
| Answer» B. rolling circle mode | |
| 9. |
There is a limit on upper size of the DNA to be packed. Choose the correct statement with respect to phages in this context. |
| A. | There is some phage DNA lost in this process |
| B. | The phages are known as transformed phages |
| C. | These type of phages can’t be selected and harvested |
| D. | Lambda is not a special attachment site |
| Answer» B. The phages are known as transformed phages | |
| 10. |
A portion of phage is removed and in place of it, the DNA of interest is inserted. This type of vector is called as __________ |
| A. | displacement vector |
| B. | insertion vector |
| C. | substitution vector |
| D. | transposition vector |
| Answer» D. transposition vector | |
| 11. |
Choose the correct statement for lambda gt10. |
| A. | It is having an EcoRI site |
| B. | It is having cII gene |
| C. | It accepts an insert of size greater than that of 8 kbp |
| D. | This size is less than that the size which is generally accepted by wild type phage |
| Answer» B. It is having cII gene | |
| 12. |
What happens once the cosmid enters the E. coli cells? |
| A. | There is strict size selection inside E. coli cells |
| B. | Partial deletion may take place |
| C. | The tendency of deletion may is increased by using low copy number |
| D. | The tendency of deletion can’t be altered |
| Answer» C. The tendency of deletion may is increased by using low copy number | |
| 13. |
Which of the following doesn’t takes place after replication? |
| A. | The single stranded sequence is generated |
| B. | It is circularized to form closed single stranded molecule |
| C. | It may circularize or remain linear |
| D. | The double stranded molecule can be synthesized by cellular DNA synthesis |
| Answer» D. The double stranded molecule can be synthesized by cellular DNA synthesis | |
| 14. |
If recombination takes place between elements in the second strain, it would replicate by the origin of replication of mini Mu. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 15. |
If colEI derived origin of replication is replaced by the origin of replication of F plasmid, it is called as: |
| A. | phagemid |
| B. | F cosmid |
| C. | plasmid |
| D. | fosmid |
| Answer» E. | |
| 16. |
How many phases are there for replication of lambda DNA to take place? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 17. |
Choose the correct statement for cosmids. |
| A. | It can be regarded as lambda substitution vector |
| B. | Less amount of phage DNA is deleted |
| C. | Only cos packaging sites are left |
| D. | It doesn’t contains a origin of replication |
| Answer» D. It doesn’t contains a origin of replication | |
| 18. |
Which of the phage genes are responsible for phage excision? |
| A. | xis gene |
| B. | int gene |
| C. | recA protein |
| D. | both xis and int gene |
| Answer» E. | |
| 19. |
The cI function can be scanned in order to check whether the recombinants are present or not. Which of the following doesn’t hold true? |
| A. | The cI protein is required for the formation of lysogen |
| B. | The plaques formed are turbid in the case if the cI gene is active |
| C. | The plaques formed are clear in the case if the cI gene is inactive |
| D. | hflA mutant host reduces the amount of cII stability |
| Answer» E. | |
| 20. |
Replication can also be carried out by rolling circle replication. Choose the correct statement for this type of replication? |
| A. | It can be used for the formation of RF |
| B. | The product of gene III binds on a specific site of double stranded genome |
| C. | It creates a nick in the – strand generating a free 3’ hydroxyl |
| D. | This strand is extended by the polymerase and displaces the original – strand |
| Answer» B. The product of gene III binds on a specific site of double stranded genome | |
| 21. |
Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it. |
| A. | This excision is possible in vivo |
| B. | Along with the F factor, host chromosome’s DNA sequences are also excited |
| C. | The sequences of host chromosome which can be accommodated in the F plasmid are small in size |
| D. | These plasmids are termed as F’ plasmids |
| Answer» D. These plasmids are termed as F’ plasmids | |
| 22. |
F (fertility) factor is used in many bacterial systems for different purposes. Choose the incorrect statement with respect to the F factor. |
| A. | It was identified in E. coli for catalysing genetic exchange between cells |
| B. | Cells having F factor are called as female |
| C. | The F factor is transferred from one cell to another via factors present outside |
| D. | They are not related to BAC systems |
| Answer» B. Cells having F factor are called as female | |
| 23. |
Selection of molecules by lack of inserts on the basis of the sacB gene is known as: |
| A. | positive selection |
| B. | negative selection |
| C. | counter selection |
| D. | sacB selection |
| Answer» D. sacB selection | |
| 24. |
Induction of lysogen takes place because of __________ |
| A. | Low levels of cII gene |
| B. | Low level of cI gene |
| C. | Low levels of both cI and cII gene |
| D. | High levels of both cI and cII gene |
| Answer» C. Low levels of both cI and cII gene | |
| 25. |
Choose the incorrect statement for cro and Q proteins. |
| A. | They are required for the lytic life cycle to continue |
| B. | Cro protein inactivates the PRM promoter |
| C. | The Q protein is responsible for the expression of late genes |
| D. | cII protein is required for the lytic life cycle to continue |
| Answer» E. | |
| 26. |
Which of the promoter requires a low level of cI gene for its activation? |
| A. | PR |
| B. | PL |
| C. | PRE |
| D. | PRM |
| Answer» E. | |
| 27. |
Choose the incorrect statement for PAC vectors. |
| A. | In these vectors, the phage packaging signals are removed but the two P1 origin of replication persist |
| B. | They resemble BAC vectors |
| C. | They accept an insert of upto 300 kbp |
| D. | Instability is observed |
| Answer» E. | |
| 28. |
What is the size of the insert that can be accommodated in the head of bacteriophage PI? |
| A. | 30-40 kbp |
| B. | 80-95 kbp |
| C. | 110-115 kbp |
| D. | 200-300 kbp |
| Answer» D. 200-300 kbp | |
| 29. |
Choose the correct statement for cII gene and its related function? |
| A. | The transcription is extended to the region of cII gene and it is termed as late transcription |
| B. | They are responsible for deactivating the promoter for repressor establishment |
| C. | This promoter is responsible to decide whether the life cycle would be lysogenic or lytic |
| D. | cII gene is responsible for other gene which is gene cIII |
| Answer» D. cII gene is responsible for other gene which is gene cIII | |
| 30. |
Choose the correct statement in respect to sacB gene? |
| A. | The cloning site for PAC lies on the side of sacB gene |
| B. | It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose |
| C. | Expression of sacB in the presence of sucrose is beneficial |
| D. | Levan is non-toxic for E. coli |
| Answer» C. Expression of sacB in the presence of sucrose is beneficial | |
| 31. |
Which gene is required for the lysogenic phage to be adopted? |
| A. | cI |
| B. | cII |
| C. | cIII |
| D. | All the three genes are interrelated |
| Answer» E. | |
| 32. |
The immediate early transcripts direct synthesis of which genes? |
| A. | N genes |
| B. | cro genes |
| C. | Both N and cro genes |
| D. | PR and PL genes |
| Answer» D. PR and PL genes | |
| 33. |
M13 vector is tightly packed. Where is the available site to insert DNA? |
| A. | In the gene II |
| B. | Between gene II and IV |
| C. | Between gene III and V |
| D. | Between gene V and VIII |
| Answer» C. Between gene III and V | |
| 34. |
M13-plasmid vectors are often used. Which of the following is not a feature for this? |
| A. | They are known as phagemid, plage or phasmid |
| B. | Replication takes place through the origin of replication of M13 derivatives only |
| C. | Replication from M13 requires proteins to be provided by helper phage |
| D. | Some of the examples of these types of the combination are pUC 118, 119 and 120 |
| Answer» C. Replication from M13 requires proteins to be provided by helper phage | |
| 35. |
Which of the statement doesn’t holds for phage display systems? |
| A. | The filamentous phage is often used as phage display system |
| B. | Coding sequences are inserted in one of the protein coating genes |
| C. | The most common protein coding genes are gene II or gene VIII |
| D. | The inserted is expressed along with other protein coat genes |
| Answer» D. The inserted is expressed along with other protein coat genes | |
| 36. |
The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________ |
| A. | plaques |
| B. | holes |
| C. | colony |
| D. | pothole |
| Answer» B. holes | |
| 37. |
What is the size of the DNA molecule which can be packed by the viral protein coat? |
| A. | 100kbp |
| B. | 200kbp |
| C. | 500kbp |
| D. | No size restriction |
| Answer» E. | |
| 38. |
The plaques formed by M13 infection are called as __________ |
| A. | retarded plaques |
| B. | true plaques |
| C. | pseudoplaques |
| D. | lysogenized plaques |
| Answer» D. lysogenized plaques | |
| 39. |
M13 doesn’t actually lyse the cells. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 40. |
What happens after packaging is carried out in the phage head? |
| A. | A sequence dependent cleavage of DNA is carried out |
| B. | A sequence independent cleavage of DNA is carried out |
| C. | They are linearized before they are infected into the bacterial cells |
| D. | They are circularized before they are infected into the bacterial cells |
| Answer» C. They are linearized before they are infected into the bacterial cells | |
| 41. |
What happens if chain-termination mutation is in the S gene? |
| A. | Cell lysis is blocked |
| B. | Growth of cells containing low levels of packaging proteins is not allowed |
| C. | The lysis of cells is not carried artificially |
| D. | Packaging is not carried out efficiently |
| Answer» B. Growth of cells containing low levels of packaging proteins is not allowed | |
| 42. |
The replication rate remains the same for all the phages irrespective of what sequence is there in the phage. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 43. |
The essential sites for recombination are known as __________ |
| A. | chi sites |
| B. | rec sites |
| C. | gam sites |
| D. | red sites |
| Answer» B. rec sites | |
| 44. |
Which size of the insert is accepted by the cosmids? |
| A. | 10-20 kbp |
| B. | 35-45 kbp |
| C. | 50-60 kbp |
| D. | 100-120 kbp |
| Answer» C. 50-60 kbp | |
| 45. |
F factor integration into the host chromosome is called as __________ |
| A. | F’ plasmid |
| B. | F factor and host chromosome recombination |
| C. | Hfr (High frequency of recombination) |
| D. | Recombinant host chromosome |
| Answer» D. Recombinant host chromosome | |
| 46. |
Which of the following is not included as a step in cloning in bacteriophage PI? |
| A. | The growth of vector is done as a plasmid by using pBR322 origin of replication |
| B. | Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated |
| C. | Incubation with mutant lysogen is done which doesn’t cleaves the recombinants at pac sites |
| D. | Incubation is done with mutant lysogen which doesn’t contains head and tail proteins |
| Answer» D. Incubation is done with mutant lysogen which doesn’t contains head and tail proteins | |
| 47. |
Choose the correct statement for RecBCD nuclease. |
| A. | It promotes rolling circle replication |
| B. | It is blocked by Gam protein |
| C. | It blocks theta mode of replication |
| D. | It is blocked by Red protein |
| Answer» C. It blocks theta mode of replication | |
| 48. |
Once the bacteriophage enters the bacterial cells, some changes are carried out in it. Which of the following is not included? |
| A. | The single stranded molecule entering the cell is single stranded and is called as a + strand |
| B. | The single stranded molecule is converted into double stranded molecule which is called as replicative form (RF) |
| C. | It is carried out in a process similar to normal replication process of E. coli |
| D. | The positive strand is used for the transcription of viral proteins |
| Answer» E. | |
| 49. |
Which of the following is the correct method to check whether the DNA has entered into the cell or not in the case of cosmid? |
| A. | If a transformation has taken place turbid plaques are formed |
| B. | If a transformation has taken place clear plaques are formed |
| C. | If a transformation has taken place, it can be confirmed via ampicillin resistance |
| D. | If a transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also |
| Answer» D. If a transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also | |
| 50. |
The red and gam genes are removed in which type of phages? |
| A. | Substitution phage |
| B. | Replacement and substitution phages both |
| C. | Replacement phage |
| D. | Substitution is preferred over replacement phage |
| Answer» D. Substitution is preferred over replacement phage | |