Explore topic-wise MCQs in Molecular Biology.

This section includes 20 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

What is the holiday junction?

A. The site of strand break
B. The site of heteroduplex DNA formation
C. Formation of a crossing over complex
D. The site of strand invasion
Answer» D. The site of strand invasion
Discussion
2.

Recombination regulates the function of genes.

A. True
B. False
Answer» B. False
Discussion
3.

Homologous recombination does not provide ____________

A. Genetic variation
B. Sequence retrieval
C. Restart of stalled replication
D. Random base incorporation
Answer» E.
Discussion
4.

When two genes on a chromosome are inherited without any record of recombination the genes are said to be ____________

A. Silenced
B. Joined
C. Coordinated
D. Linked
Answer» E.
Discussion
5.

The frequency of crossing over between two genes on the same chromosome is independent of their position.

A. True
B. False
Answer» C.
Discussion
6.

Homologous recombination in germ cells occur in the ____________ phase.

A. Leptotene
B. Zygotene
C. Pachytene
D. Diplotene
Answer» D. Diplotene
Discussion
7.

THE_FREQUENCY_OF_CROSSING_OVER_BETWEEN_TWO_GENES_ON_A_SAME_CHROMOSOME_IS_INDEPENDENT_OF_THEIR_POSITION.?$

A. True
B. False
Answer» C.
Discussion
8.

Homologous recombination does not provide ____________$

A. Genetic variation
B. Sequence retrieval
C. Restart of stalled replication
D. Random base incorporation
Answer» E.
Discussion
9.

When_two_genes_on_a_chromosome_is_inherited_without_any_record_of_recombination_the_genes_are_said_to_be_____________$

A. Silenced
B. Joined
C. Coordinated
D. Linked
Answer» E.
Discussion
10.

What is holiday junction?

A. The site of strand break
B. The site of heteroduplex DNA formation
C. Formation of a crossing over complex
D. The site of strand invasion
Answer» D. The site of strand invasion
Discussion
11.

What is strand invasion?

A. Fork collapse
B. Nick in template strand
C. Formation of lesion
D. Complementary strand pairing
Answer» E.
Discussion
12.

Recombination_regulates_the_function_of_genes.

A. True
B. False
Answer» B. False
Discussion
13.

Homologous recombination in germ cells occur in the ____________ phase?

A. Leptotene
B. Zygotene
C. Pachytene
D. Diplotene
Answer» D. Diplotene
Discussion
14.

Which polymerase is used in PCR based mutagenesis?

A. Deep vent R polymerase
B. pfu polymerase
C. Taq polymerase
D. DNA polymerase
Answer» C. Taq polymerase
Discussion
15.

Which two genes are absent in the E. coli strain CJ236?

A. dut-/Ung-
B. Rec-/RecB-
C. duB-/Ung-
D. dut-/Umg-
Answer» B. Rec-/RecB-
Discussion
16.

Write down the name of scientist who has discovered the method of site directed mutagenesis?

A. Bostein Shortle
B. Craik
C. Grait
D. Joller Smith
Answer» E.
Discussion
17.

Which phage is used in oligonucleotide directed mutagenesis?

A. M13
B. Cosmid
C. Phagemid
D. λ – phage
Answer» B. Cosmid
Discussion
18.

What is the function of Dpm I endonuclease in Tm method of site directed mutagenesis?

A. Joining of blunt ends
B. Addition of dNTPs
C. Sensitive to cleavage of methylated DNA
D. Breaking of DNA strand
Answer» D. Breaking of DNA strand
Discussion
19.

What is the formula for annealing temperature for polymerase chain reaction primer?

A. Tm = 81.5 + 0.41 (%AT) – (675/N)
B. Tm = 81.5 + 0.41 (%GC) – (675/N)
C. Tm = 81.2 + 0.41 (%GC) – (672/N)
D. Tm = 81.5 + 0.42 (%AT) – (675/N)
Answer» C. Tm = 81.2 + 0.41 (%GC) ‚Äö√Ñ√∂‚àö√ë‚àö¬® (672/N)
Discussion
20.

Which of the following properties is improved by site directed mutagenesis?

A. Physical property
B. Chemical property
C. Kinetic property
D. Integrity
Answer» D. Integrity
Discussion