MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Pulse field gel electrophoresis was developed by ____________ |
| A. | Collins and John |
| B. | Kary Mullis |
| C. | Patrick O’ Farrell |
| D. | Schwartz and Cantor |
| Answer» E. | |
| 2. |
Which of the following is not a character of polyacrylamide gel? |
| A. | Inert |
| B. | Ionic strength |
| C. | Stable over a wide range of pH |
| D. | Separate upto a few 100 bp of DNA |
| Answer» E. | |
| 3. |
Electrophoresis cannot be used to separate _______________ |
| A. | DNA |
| B. | RNA |
| C. | Amino acid |
| D. | Protein |
| Answer» D. Protein | |
| 4. |
WHICH_OF_THE_FOLLOWING_IS_NOT_A_CHARACTER_OF_POLYACRYLAMIDE_GEL??$ |
| A. | Inert |
| B. | Ionic strength |
| C. | Stable over a wide range of pH |
| D. | Separate upto a few 100 bp of DNA |
| Answer» E. | |
| 5. |
Pulse_field_gel_electrophoresis_was_developed_by_____________$ |
| A. | Collins and John |
| B. | Kary Mullis |
| C. | Patrick O’ Farrell |
| D. | Schwartz and Cantor |
| Answer» E. | |
| 6. |
Electrophoresis cannot be used to separate ______________? |
| A. | DNA |
| B. | RNA |
| C. | Amino acid |
| D. | Protein |
| Answer» D. Protein | |
| 7. |
Agarose can be extracted from which of the following? |
| A. | Gracilaria esculentus |
| B. | Lycazusican esculentum |
| C. | Ficum benghalensis |
| D. | Agrostis stolonifera |
| Answer» B. Lycazusican esculentum | |
| 8. |
Which of the following will migrate faster? The condition is the molecular weight of the following is equal. |
| A. | Supercoiled circular DNA |
| B. | Nicked circular DNA |
| C. | Single stranded DNA |
| D. | Double stranded DNA |
| Answer» B. Nicked circular DNA | |
| 9. |
Pulse field gel electrophoresis separates DNA molecules of size ___________ |
| A. | 10 – 20 bp |
| B. | 20 – 30 Kb |
| C. | 30 – 50 Kb |
| D. | 40 – 50 bp |
| Answer» D. 40 ‚Äö√Ñ√∂‚àö√ë‚àö¬® 50 bp | |
| 10. |
The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA? |
| A. | Stacked between histone molecules |
| B. | Binds to the nucleotide base |
| C. | Intercalated between the stacked bases |
| D. | Binds to the phosphodiester backbone |
| Answer» D. Binds to the phosphodiester backbone | |
| 11. |
If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis? |
| A. | 3 |
| B. | 4 |
| C. | 5 |
| D. | 6 |
| Answer» C. 5 | |
| 12. |
The polymerization of the gel used in PAGE occurs between polyacrylamide and ____________ |
| A. | N, N – acrylamide |
| B. | Bisacrylamide |
| C. | N – methyleneacrylamide |
| D. | N, N – methylene bisacrylamide |
| Answer» E. | |
| 13. |
Which of the following cannot be used for the separation of nucleic acids? |
| A. | SDS – PAGE |
| B. | PAGE |
| C. | Northern blotting |
| D. | PAGE |
| Answer» B. PAGE | |