MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
________________ is a modification of RT – PCR. |
| A. | Overlap extension PCR |
| B. | Inverse PCR |
| C. | Thermal cycle sequencing PCR |
| D. | RACE |
| Answer» E. | |
| 2. |
The cDNA library provides several advantages over RT – PCR. |
| A. | True |
| B. | False |
| Answer» C. | |
| 3. |
The digestion of mRNA during RT – PCR is carried out by the enzyme ____________ |
| A. | Exonuclease |
| B. | RNase H |
| C. | Bal 31 |
| D. | Endonuclease |
| Answer» C. Bal 31 | |
| 4. |
In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________ |
| A. | 5’ end of RNA |
| B. | 3’ end of RNA |
| C. | 3’ end of cDNA |
| D. | 5’ end of cDNA |
| Answer» D. 5’ end of cDNA | |
| 5. |
Which of the following serves as the first primer in RT – PCR for eukaryotic RNA? |
| A. | Oligo A |
| B. | Oligo T |
| C. | Oligo G |
| D. | Oligo C |
| Answer» C. Oligo G | |
| 6. |
Reverse transcriptase produces DNA from RNA. |
| A. | True |
| B. | False |
| Answer» C. | |
| 7. |
________________ is a modification of RT – PCR?# |
| A. | Overlap extension PCR |
| B. | Inverse PCR |
| C. | Thermal cycle sequencing PCR |
| D. | RACE |
| Answer» E. | |
| 8. |
The cDNA library provides several advantages over RT – PCR _________$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 9. |
From which organism is the enzyme reverse transcriptase isolated? |
| A. | Bacteria |
| B. | Fungi |
| C. | Virus |
| D. | Prions |
| Answer» D. Prions | |
| 10. |
How many primers are used in the process of reverse transcriptase amplification? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 11. |
The digestion of mRNA during RT – PCR is carried out by the enzyme ____________$ |
| A. | Exonuclease |
| B. | RNase H |
| C. | Bal 31 |
| D. | Endonuclease |
| Answer» C. Bal 31 | |
| 12. |
In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________$ |
| A. | 5’ end of RNA |
| B. | 3’ end of RNA |
| C. | 3’ end of cDNA |
| D. | 5’ end of cDNA |
| Answer» D. 5‚Äö√Ñ√∂‚àö√ë‚àö¬• end of cDNA | |
| 13. |
Which of the following serves as the first primer in RT – PCR for eukaryotic RNA?$ |
| A. | Oligo A |
| B. | Oligo T |
| C. | Oligo G |
| D. | Oligo C |
| Answer» C. Oligo G | |
| 14. |
Reverse transcriptase produces DNA from RNA _______ |
| A. | True |
| B. | False |
| Answer» C. | |