MCQOPTIONS
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MCQOPTIONS
This section includes 20 Mcqs, each offering curated multiple-choice questions to sharpen your Gene Manipulation knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Rad54-3 allele stabilizes clones containing ____________ |
| A. | Plant DNA |
| B. | Viral DNA |
| C. | Human DNA |
| D. | Bacterial DNA |
| Answer» D. Bacterial DNA | |
| 2. |
Rad52 mutated strains grow slowly than RAD strains. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 3. |
Rad52 mutation can reduce the frequency of _________ in YACs. |
| A. | Temperature change |
| B. | Deletions |
| C. | Mutations |
| D. | Hybridization |
| Answer» C. Mutations | |
| 4. |
Deletions in the clones of YAC vectors can occur both during transformation process and _______________ of transformants. |
| A. | Meiotic division |
| B. | Mitotic growth |
| C. | Ligation |
| D. | Digestion |
| Answer» C. Ligation | |
| 5. |
Many clones of the YACs are __________ |
| A. | Contaminated |
| B. | Small |
| C. | Unstable |
| D. | Indistinguishable |
| Answer» D. Indistinguishable | |
| 6. |
RAD52_MUTATION_CAN_REDUCE_THE_FREQUENCY_OF___________IN_YACS.?$ |
| A. | Temperature change |
| B. | Deletions |
| C. | Mutations |
| D. | Hybridization |
| Answer» C. Mutations | |
| 7. |
Rad54-3 allele stabilizes clones containing ____________$ |
| A. | Plant DNA |
| B. | Viral DNA |
| C. | Human DNA |
| D. | Bacterial DNA |
| Answer» D. Bacterial DNA | |
| 8. |
Rad52 mutated strains grow slowly than RAD strains.$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
How can YAC DNA be purified from yeast chromosomes? |
| A. | Gel electrophoresis |
| B. | CHEF |
| C. | Centrifugation |
| D. | PFGE |
| Answer» E. | |
| 10. |
There is a high rate of loss of some YACs during Translation |
| A. | Transcription |
| B. | Mitotic growth |
| C. | Meiosis |
| D. | Promoters |
| Answer» D. Promoters | |
| 11. |
Which of the following is stimulated by the spheroplasting step of YAC transformation system? |
| A. | Mutations |
| B. | Chimera |
| C. | Endotoxins |
| D. | Promoters |
| Answer» C. Endotoxins | |
| 12. |
Deletions in the clones of YAC vectors can occur both during transformation process and _______________ of transformants? |
| A. | Meiotic division |
| B. | Mitotic growth |
| C. | Ligation |
| D. | Digestion |
| Answer» C. Ligation | |
| 13. |
Many clones of the YACs are _________? |
| A. | Contaminated |
| B. | Small |
| C. | Unstable |
| D. | Indistinguishable |
| Answer» D. Indistinguishable | |
| 14. |
Chimeras can possibly be detected by which technique? |
| A. | Hybridization |
| B. | Electroporation |
| C. | UV radiation |
| D. | Spectroscopy |
| Answer» B. Electroporation | |
| 15. |
Chimeras in YAC libraries may arise due to _________ |
| A. | Increased temperature |
| B. | Poor handling |
| C. | Inactivated enzymes |
| D. | Co-ligation |
| Answer» E. | |
| 16. |
10-60% of clones in existing YAC libraries represent _______________ |
| A. | Hybridization |
| B. | Improper annealing |
| C. | Chimeric sequences |
| D. | Inactivated genes |
| Answer» D. Inactivated genes | |
| 17. |
Polyamines prevent _____________ |
| A. | RNA degradation |
| B. | DNA degradation |
| C. | Hybridization |
| D. | Protein degradation |
| Answer» C. Hybridization | |
| 18. |
Average insert size can be increased of a YAC vector by which of the following techniques? |
| A. | PFGE |
| B. | CHEF |
| C. | PCR |
| D. | Electroporation |
| Answer» B. CHEF | |
| 19. |
Less than what size of YAC is its centromere function impaired? |
| A. | 2 kb |
| B. | 10 kb |
| C. | 20 kb |
| D. | 5 kb |
| Answer» D. 5 kb | |
| 20. |
Which of the following can be used to clone very large fragments of DNA? |
| A. | M13 |
| B. | Lambda phage |
| C. | Agrobacterium |
| D. | Yeast artificial chromosome |
| Answer» E. | |