MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 18 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The process by which a probe is used to screen a library is known as ___________ |
| A. | Hybridization |
| B. | Southern blotting |
| C. | Colony hybridization |
| D. | Western blotting |
| Answer» D. Western blotting | |
| 2. |
Northern blotting is performed for ____________ |
| A. | Determining the size of DNA |
| B. | Determining the size of RNA |
| C. | Quantification of RNA |
| D. | Sequencing of RNA |
| Answer» D. Sequencing of RNA | |
| 3. |
In case of northern blotting the hybridization occurs between two RNA molecules. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
During electrophoresis denaturation of the double stranded DNA is brought about by __________ |
| A. | Treatment with alkali |
| B. | Application of current |
| C. | Treatment with EtBr |
| D. | Application of heat |
| Answer» B. Application of current | |
| 5. |
Radiolabelling is generally brought about by addition of radioactive phosphorus. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
Fluorescent labeling is an important phenomenon in case of hybridization and manipulations in molecular biology. |
| A. | True |
| B. | False |
| Answer» C. | |
| 7. |
DURING_ELECTROPHORESIS_DENATURATION_OF_THE_DOUBLE_STRANDED_DNA_IS_BROUGHT_ABOUT_BY___________?$ |
| A. | Treatment with alkali |
| B. | Application of current |
| C. | Treatment with EtBr |
| D. | Application of heat |
| Answer» B. Application of current | |
| 8. |
Northern blotting is performed for ____________$ |
| A. | Determining the size of DNA |
| B. | Determining the size of RNA |
| C. | Quantification of RNA |
| D. | Sequencing of RNA |
| Answer» D. Sequencing of RNA | |
| 9. |
In_case_of_northern_blotting_the_hybridization_occurs_between_two_RNA_molecules__________$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 10. |
The_process_by_which_a_probe_is_used_to_screen_a_library_is_known_as____________ |
| A. | Hybridization |
| B. | Southern blotting |
| C. | Colony hybridization |
| D. | Western blotting |
| Answer» D. Western blotting | |
| 11. |
Radiolabelling is generally brought about by addition of radioactive phosphorus __________? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 12. |
Fluorescent labeling is an important phenomenon in case of hybridization and manipulations in molecular biology____________ |
| A. | True |
| B. | False |
| Answer» C. | |
| 13. |
What is the function of polynucleotide kinase? |
| A. | Addition of γ – phosphate at 3’ – OH. |
| B. | Addition of γ – phosphate at 5’ – OH. |
| C. | Removal of γ – phosphate at 3’ – OH. |
| D. | Removal of γ – phosphate at 5’ – OH. |
| Answer» C. Removal of ‚âà√≠‚Äö√¢‚Ä¢ ‚Äö√Ñ√∂‚àö√ë‚àö¬® phosphate at 3‚Äö√Ñ√∂‚àö√ë‚àö¬• ‚Äö√Ñ√∂‚àö√ë‚àö¬® OH. | |
| 14. |
In how many ways a DNA can be labeled? |
| A. | 2 |
| B. | 3 |
| C. | 4 |
| D. | 5 |
| Answer» B. 3 | |
| 15. |
Why is a probe labeled? |
| A. | Improve visibility |
| B. | Improve stability |
| C. | Improve location identification |
| D. | Improve binding capability |
| Answer» D. Improve binding capability | |
| 16. |
What is a probe? |
| A. | Chemically synthesized DNA |
| B. | Purified DNA |
| C. | Fragmented DNA duplex |
| D. | Either purified or synthesized single stranded DNA |
| Answer» E. | |
| 17. |
Which of the following is not termed as hybridization? |
| A. | DNA and cDNA |
| B. | DNA and mRNA |
| C. | DNA from different species |
| D. | DNA from male and female of same species |
| Answer» E. | |
| 18. |
Which of the following does not affect the formation of hybrid DNA? |
| A. | Ionic strength |
| B. | Pressure |
| C. | Temperature |
| D. | Homologous DNA |
| Answer» C. Temperature | |