MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 23 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The repressor for the β-galactosidase gene is encoded by ___________ |
| A. | XmaI |
| B. | PstI |
| C. | Lac I |
| D. | PvuII |
| Answer» D. PvuII | |
| 2. |
Which one of the following is the first engineered plasmid vector? |
| A. | pBR322 |
| B. | pBR320 |
| C. | pUC18 |
| D. | pSC101 |
| Answer» E. | |
| 3. |
What is the characteristic of lacZ gene of pUC18 vector among the following? |
| A. | Encodes for antibiotic resistance |
| B. | Encodes for β-galactosidase enzyme |
| C. | Encodes for β-lactamase enzyme |
| D. | Encodes for β-galactoside transferase enzyme. |
| Answer» C. Encodes for β-lactamase enzyme | |
| 4. |
Which gene in the pUC18 vector confers antibiotic resistance to the transformed cells? |
| A. | LacZ |
| B. | PvuII |
| C. | AmpR |
| D. | AntII |
| Answer» D. AntII | |
| 5. |
What is the temperature at which bacteria can takes up the plasmid? |
| A. | 42˚C |
| B. | -42˚C |
| C. | 40˚C |
| D. | -40˚C |
| Answer» C. 40˚C | |
| 6. |
The host bacterium takes up a plasmid in presence of ______________ |
| A. | Monovalent cations |
| B. | Monovalent anions |
| C. | Divalent cations |
| D. | Divalent anions |
| Answer» D. Divalent anions | |
| 7. |
What is the length of the polylinker segment of plasmid? |
| A. | Less than 100 base pair |
| B. | Less than 10 base pair |
| C. | Less than 70 base pair |
| D. | Less than 50 base pair |
| Answer» B. Less than 10 base pair | |
| 8. |
Under relaxed conditions how many copies of plasmid are present in the cell? |
| A. | 10 – 100 copies |
| B. | 100 – 500 copies |
| C. | 1 – 300 copies |
| D. | 10 – 700 copies |
| Answer» E. | |
| 9. |
WHICH_GENE_IN_THE_PUC18_VECTOR_CONFERS_ANTIBIOTIC_RESISTANCE_TO_THE_TRANSFORMED_CELLS??$ |
| A. | LacZ |
| B. | PvuII |
| C. | AmpR |
| D. | AntII |
| Answer» D. AntII | |
| 10. |
WHAT_IS_THE_TEMPERATURE_AT_WHICH_BACTERIA_CAN_TAKES_UP_THE_PLASMID??$ |
| A. | 42ÀöC |
| B. | -42ÀöC |
| C. | 40ÀöC |
| D. | -40ÀöC |
| Answer» C. 40‚àö√Ñ‚àö‚àÇC | |
| 11. |
What is the characteristic of lacZ gene of pUC18 vector among the following?$ |
| A. | Encodes for antibiotic resistance |
| B. | Encodes for β-galactosidase enzyme |
| C. | Encodes for β-lactamase enzyme |
| D. | Encodes for β-galactoside transferase enzyme. |
| Answer» C. Encodes for ‚âà√≠‚Äö√¢¬ß-lactamase enzyme | |
| 12. |
The repressor for the β-galactosidase gene is encoded by ___________$ |
| A. | XmaI |
| B. | PstI |
| C. | Lac I |
| D. | PvuII |
| Answer» D. PvuII | |
| 13. |
What is incorrect about plasmid? |
| A. | Helps in reproduction |
| B. | Contains stress resistant genes |
| C. | Serves as the transformation vehicle |
| D. | They are the genetic material of the bacteria |
| Answer» E. | |
| 14. |
What is the expanded form of pBR in pBR322? |
| A. | Plasmid Boliver and Rodriguez |
| B. | Plasmid Baltimore and Rodriguez |
| C. | Plasmid bacterial recombination |
| D. | Plasmid bacterial replication |
| Answer» B. Plasmid Baltimore and Rodriguez | |
| 15. |
Which_one_of_the_following_is_the_first_engineered_plasmid_vector? |
| A. | pBR322 |
| B. | pBR320 |
| C. | pUC18 |
| D. | pSC101 |
| Answer» E. | |
| 16. |
The host bacterium takes up a plasmid in presence of _____________? |
| A. | Monovalent cations |
| B. | Monovalent anions |
| C. | Divalent cations |
| D. | Divalent anions |
| Answer» D. Divalent anions | |
| 17. |
The full for of pUC is polylinker university cloning. |
| A. | True |
| B. | False |
| Answer» C. | |
| 18. |
What is length of the polylinker segment of plasmid? |
| A. | Less than 100 base pair |
| B. | Less than 10 base pair |
| C. | Less than 70 base pair |
| D. | Less than 50 base pair |
| Answer» B. Less than 10 base pair | |
| 19. |
Under relaxed condition how many copies of plasmid are present in the cell? |
| A. | 10 – 100 copies |
| B. | 100 – 500 copies |
| C. | 1 – 300 copies |
| D. | 10 – 700 copies |
| Answer» E. | |
| 20. |
Who were the scientists who discovered the plasmid pBR322? |
| A. | Rodriguez and Bolivar |
| B. | Joller smith |
| C. | Herbert Boyer |
| D. | Stanley Cohen and Joller smith |
| Answer» B. Joller smith | |
| 21. |
How many restriction sites are contained by a plasmid? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | More than 1 |
| Answer» E. | |
| 22. |
How the plasmid clones can be screened? |
| A. | By selectable markers |
| B. | By bacterial resistance gene |
| C. | For restriction site |
| D. | By ARS sequence |
| Answer» B. By bacterial resistance gene | |
| 23. |
Plasmid replication is dependent on the host cell. |
| A. | True |
| B. | False |
| Answer» C. | |