Explore topic-wise MCQs in Molecular Biology.

This section includes 27 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture.

A. True
B. False
Answer» C.
Discussion
2.

Strand separation for DNA sequencing is done to ____________

A. sort the fragments according to their size
B. sort the fragments according to their molecular weight
C. obtain consecutive sequence as per termination
D. obtain specific lanes of sequencing
Answer» D. obtain specific lanes of sequencing
Discussion
3.

The four reaction mixtures that were prepared, contained ____________

A. dCTP, dTTP, dGTP, dATP
B. dCTP, ddTTP, dGTP, ddATP
C. ddCTP, dTTP, ddGTP, dATP
D. dCTP, ddTTP, dGTP, dATP
Answer» E.
Discussion
4.

With respect to Sanger’s enzymatic method of sequencing pick the odd one out.

A. Radioactive dideoxyribonucleotides
B. Primers
C. Klenow fragment
D. Restriction digestion
Answer» E.
Discussion
5.

Which enzyme is used for the replication in case of Sanger’s method of sequencing?

A. Polymerase I
B. Smaller subunit polymerase I
C. Polymerase III
D. Larger subunit polymerase I
Answer» E.
Discussion
6.

The dideoxy method is also known as _____________

A. Maxem and Gilbert method
B. Autosequencing
C. Sanger’s enzymatic sequencing
D. Pyrosequencing
Answer» D. Pyrosequencing
Discussion
7.

The process of electrophoresis is the key to sequencing.

A. True
B. False
Answer» B. False
Discussion
8.

Which step is not involved in base-specific cleavage of DNA fragment?

A. Modification of concerned base
B. Removal of modified base from DNA strand
C. Induction of random strand break
D. End labeled DNA fragments of variable lengths produced
Answer» D. End labeled DNA fragments of variable lengths produced
Discussion
9.

What are the basic base-specific cleavage sites used in Maxem and Gilbert method?

A. A, T, G, C
B. C, T, A+G, T+C
C. A, G, A+T, G+C
D. G, C, A+G, C+T
Answer» E.
Discussion
10.

The 32P is added at the 3’ end by polynucleotidyl kinase.

A. True
B. False
Answer» C.
Discussion
11.

End labeled DNA sequencing is known as dideoxy method of sequencing.

A. True
B. False
Answer» C.
Discussion
12.

THE_PROCESS_OF_ELECTROPHORESIS_IS_THE_KEY_TO_SEQUENCING.?$

A. True
B. False
Answer» B. False
Discussion
13.

Which enzyme is used for the replication in case of Sanger’s method of sequencing?$#

A. Polymerase I
B. Smaller subunit polymerase I
C. Polymerase III
D. Larger subunit polymerase I
Answer» E.
Discussion
14.

The_dideoxy_method_is_also_known_as_……………………………$#

A. Maxem and Gilbert method
B. Autosequencing
C. Sanger’s enzymatic sequencing
D. Pyrosequencing
Answer» D. Pyrosequencing
Discussion
15.

The position of band during electrophoresis indicates the dNTP used as chain terminator in that mixture.

A. True
B. False
Answer» C.
Discussion
16.

Strand separation for DNA sequencing is done to ……………………………$

A. sort the fragments according to their size
B. sort the fragments according to their molecular weight
C. obtain consecutive sequence as per termination
D. obtain specific lanes of sequencing
Answer» D. obtain specific lanes of sequencing
Discussion
17.

ddNTP acts as the chain radioactive markers.

A. True
B. False
Answer» C.
Discussion
18.

The four reaction mixtures that were prepared, contained ……………………………$

A. dCTP, dTTP, dGTP, dATP
B. dCTP, ddTTP, dGTP, ddATP
C. ddCTP, dTTP, ddGTP, dATP
D. dCTP, ddTTP, dGTP, dATP
Answer» E.
Discussion
19.

With_respect_to_Sanger’s_enzymatic_method_of_sequencing_pick_the_odd_one_out.$

A. Radioactive dideoxyribonucleotides
B. Primers
C. Klenow fragment
D. Restriction digestion
Answer» E.
Discussion
20.

Which step is not involved in base – specific cleavage of DNA fragment?#

A. Modification of concerned base
B. Removal of modified base from DNA strand
C. Induction of random strand break
D. End labeled DNA fragments of variable lengths produced
Answer» D. End labeled DNA fragments of variable lengths produced
Discussion
21.

What are the basic base – specific cleavage sites used in Maxem and Gilbert method?$

A. A, T, G, C
B. C, T, A+G, T+C
C. A, G, A+T, G+C
D. G, C, A+G, C+T
Answer» E.
Discussion
22.

The denatured strands cannot be separated.

A. True
B. False
Answer» C.
Discussion
23.

Restriction digestion is the only process to achieve sequencing by the Maxem and Gilbert method.

A. True
B. False
Answer» C.
Discussion
24.

The end labeled fragment is cleaved in how many pieces?

A. 2
B. 3
C. 4
D. 5
Answer» B. 3
Discussion
25.

The 32P is added at the 3’ end by polynucleotidyl kinase$

A. True
B. False
Answer» C.
Discussion
26.

End labeled DNA sequencing is known as dideoxy method of sequencing

A. True
B. False
Answer» C.
Discussion
27.

Which of the following is not required for DNA sequencing?

A. Restriction digestion
B. Electrophoresis
C. Cloning
D. Polymerase chain reaction
Answer» D. Polymerase chain reaction
Discussion