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This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The gaps formed by the hydrolyzing the RNA from RNA:DNA hybrid is filled by DNA polymerase I and the nicks are ligated by T4 ligase. |
| A. | True |
| B. | False |
| Answer» C. | |
| 2. |
Which of the following is not used for degrading RNA from RNA:DNA hybrid in replication? |
| A. | RNase A |
| B. | RNase H |
| C. | Polymerase I |
| D. | Exonuclease |
| Answer» B. RNase H | |
| 3. |
Primer synthesis short stretches of DNA for replication. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
WHICH_OF_THE_FOLLOWING_IS_NOT_USED_FOR_DEGRADING_RNA_FROM_RNA:DNA_HYBRID_IN_REPLICATION??$ |
| A. | RNase A |
| B. | RNase H |
| C. | Polymerase I |
| D. | Exonuclease |
| Answer» B. RNase H | |
| 5. |
The_gaps_formed_by_the_hydrolyzing_the_RNA_from_RNA:DNA_hybrid_is_filled_by_DNA_polymerase_I_and_the_nicks_are_ligated_by_T4_ligase.$ |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
Primer synthesis short stretches of DNA for replication? |
| A. | True |
| B. | False |
| Answer» C. | |
| 7. |
In the case of a circular DNA synthesis how many replication forks are observed? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 8. |
Who was the first person to analyse the process of replication and on which organism? |
| A. | Arthur Kornberg: E. coli |
| B. | John Cairns: E. coli |
| C. | Arthur Kornberg: Bacillus subtilis |
| D. | John Cairns: Bacillus subtilis |
| Answer» C. Arthur Kornberg: Bacillus subtilis | |
| 9. |
Which of the following does not affect DNA replication? |
| A. | Antiparallel nature of DNA |
| B. | End specificity of polymerase |
| C. | SSB protein |
| D. | Helicase |
| Answer» D. Helicase | |
| 10. |
Replication fork is the junction between the two ___________ |
| A. | Unreplicated DNA |
| B. | Newly synthesized DNA |
| C. | Newly separated DNA strands and newly synthesized DNA strands |
| D. | Newly separated DNA strands and the unreplicated DNA |
| Answer» E. | |
| 11. |
Which of the following is correctly matched with its subsequent role? |
| A. | Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex |
| B. | Polymerase I- larger fragment responsible for exonuclease activity |
| C. | DnaA protein- responsible for “melting” of the DNA double helix during replication |
| D. | DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand |
| Answer» D. DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand | |
| 12. |
Pick the correct pair with respect to primers used in DNA replication. |
| A. | RNA primer- for prokaryotes only |
| B. | DNA primer-for eukaryotes only |
| C. | DNA primer- for both prokaryotes and eukaryotes |
| D. | RNA primer- for both prokaryotes and eukaryotes |
| Answer» E. | |
| 13. |
Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________ |
| A. | Walter Flemming on root tip cells of Vicia faba |
| B. | J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba |
| C. | Walter Flemming on root tip cells of Phaseolus vulgaris |
| D. | J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Phaseolus vulgaris |
| Answer» C. Walter Flemming on root tip cells of Phaseolus vulgaris | |