MCQOPTIONS
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MCQOPTIONS
This section includes 20 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What is the significance of “c” in cDNA library? |
| A. | Copy DNA |
| B. | Cut DNA |
| C. | Cell DNA |
| D. | Clone DNA |
| Answer» B. Cut DNA | |
| 2. |
The ordered steps for the construction of library involves __________ |
| A. | iv, v, i, iii, ii |
| B. | v, iv, i, iii, ii |
| C. | i, ii, iii, iv, v |
| D. | ii, iii, iv, v, iView Answer |
| Answer» B. v, iv, i, iii, ii | |
| 3. |
Partial digestion is preferred for the fragmentation of DNA. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
Which enzyme is used to avoid ligation of separate DNA? |
| A. | Kinase |
| B. | Phosphatase |
| C. | Endonuclease |
| D. | Ligase |
| Answer» C. Endonuclease | |
| 5. |
What is the state of phage cloning vector that is used for the construction of library? |
| A. | Packaged phage |
| B. | Unpackaged phage |
| C. | Packaged and unpackaged phage |
| D. | Packaged and unpackaged phage are used but unpackaged phage is favored |
| Answer» C. Packaged and unpackaged phage | |
| 6. |
WHAT_IS_THE_STATE_OF_PHAGE_CLONING_VECTOR_THAT_IS_USED_FOR_THE_CONSTRUCTION_OF_LIBRARY??$ |
| A. | Packaged phage |
| B. | Unpackaged phage |
| C. | Packaged and unpackaged phage |
| D. | Packaged and unpackaged phage are used but unpackaged phage is favored |
| Answer» C. Packaged and unpackaged phage | |
| 7. |
Partial digestion is preferred for the fragmentation of DNA.$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Which_enzyme_is_used_to_avoid_ligation_of_separate_DNA?$ |
| A. | Kinase |
| B. | Phosphatase |
| C. | Endonuclease |
| D. | Ligase |
| Answer» C. Endonuclease | |
| 9. |
What is the significance of SI nuclease? |
| A. | DNA synthesis |
| B. | Cleavage of hooks |
| C. | Degrades RNA |
| D. | Annealing the primer |
| Answer» C. Degrades RNA | |
| 10. |
mRNA can be readily separated from lysed eukaryotic cells adding magnetic beads. What is the sequence of this magnetic bead? |
| A. | Oligo G |
| B. | Oligo A |
| C. | Oligo T |
| D. | Oligo C |
| Answer» D. Oligo C | |
| 11. |
What is the significance of “c” in cDNA library?$ |
| A. | Copy DNA |
| B. | Cut DNA |
| C. | Cell DNA |
| D. | Clone DNA |
| Answer» B. Cut DNA | |
| 12. |
The_ordered_steps_for_the_construction_of_library_involves___________ |
| A. | Vector preparation |
| B. | Amplification |
| C. | Ligation and introduction into the host |
| D. | Isolation of genomic DNA |
| Answer» B. Amplification | |
| 13. |
What is the characteristic of the DNA that is used for the construction of library? |
| A. | Naked DNA |
| B. | Plasmid |
| C. | Plasmid and naked DNA |
| D. | Plasmid is preferred over naked DNA |
| Answer» D. Plasmid is preferred over naked DNA | |
| 14. |
During storage of DNA fragments for making library, larger fragments are having more chances of getting lost. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 15. |
cDNA is synthesized from __________ |
| A. | Protein chain |
| B. | tRNA |
| C. | mRNA |
| D. | DNA |
| Answer» D. DNA | |
| 16. |
Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid? |
| A. | RNase A |
| B. | RNase H |
| C. | DNase |
| D. | Exonuclease |
| Answer» C. DNase | |
| 17. |
For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector? |
| A. | Less than 100 bases |
| B. | 100 to 1000 bases |
| C. | Less than 100 to mega bases |
| D. | Less than 10 bases |
| Answer» D. Less than 10 bases | |
| 18. |
Which enzyme is involved in the synthesis of the DNA over an RNA template? |
| A. | DNA polymerase |
| B. | Reverse transcriptase |
| C. | Klenow fragment |
| D. | RNA polymerase |
| Answer» C. Klenow fragment | |
| 19. |
In the following type of libraries pick the odd one out. |
| A. | A DNA library |
| B. | B DNA library |
| C. | cDNA library |
| D. | Z DNA library |
| Answer» D. Z DNA library | |
| 20. |
How many types of DNA libraries are possible? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |