MCQOPTIONS
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MCQOPTIONS
This section includes 63 Mcqs, each offering curated multiple-choice questions to sharpen your Bioinformatics knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
In Grid representation, in a translational scan the mobile molecule B moves through the grid representing the static molecule A and a signal describing shape complementarity, fc, is generated for each mapping. Mathematically the correlation function, fc, of fA and fB is given by ______ where N is the number of grid points along the cubic axes i, j, and k and α, β, and γ are the translational vectors of the mobile molecule B relative to the static one A. |
| A. | fc = \(\sum_{i=1,j=1,k=1}^N\) (fA,i,j,k * fBi+αj+βk+ɣ) |
| B. | fc = \(\sum_{i=0,j=0,k=0}^N\) (fA,i,j,k * fBi+αj+βk+ɣ) |
| C. | fc = \(\sum_{i=1,j=1,k=1}^N\) (fA,0,0,0 * fBi+αj+βk+ɣ) |
| D. | fc = \(\sum_{i=0,j=0,k=0}^N\) (f0,i,j,k * fBi+αj+βk+ɣ) |
| Answer» B. fc = \(\sum_{i=0,j=0,k=0}^N\) (fA,i,j,k * fBi+αj+βk+ɣ) | |
| 52. |
The burial of surface area (or the maximization of surface contact) is an approximation of the effect of shape complementarity. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 53. |
Which of the following is untrue about Shape Complementarity? |
| A. | The complementarity between two proteins or a protein and ligand can be described by surface contact or overlap |
| B. | The complementarity between two proteins or a protein and ligand can be described by the overall buried surface area of two molecules in contact |
| C. | The complementarity between two proteins or a protein and ligand can be described by the number of adjacent surface points (atoms or residues) |
| D. | The hydrophobic effect barely has impact on protein folding |
| Answer» E. | |
| 54. |
Which of the following is untrue about Amino acid conservation? |
| A. | It has been known for some time that conservation of residues at the surface of a protein family is often related to function |
| B. | Conservation of residues at the core of a protein family is often related to function |
| C. | This may be an enzyme active-site or binding site |
| D. | Unlike hydrophobicity or electrostatic potential, displaying residue conservation on the molecular surface has no physical or chemical basis |
| Answer» C. This may be an enzyme active-site or binding site | |
| 55. |
WHICH_OF_THE_FOLLOWING_IS_INCORRECT_ABOUT_PURIFICATION_OF_PROTEIN_COMPLEXES_FOLLOWED_BY_MASS_SPECTROMETRY??$ |
| A. | Isolating protein complexes from cells allows identification of interactions between ensembles of proteins instead of just pairs |
| B. | Systematic purification of complexes on a large scale is done by tagging hundreds of genes with an epitope |
| C. | UnLike in the yeast-two-hybrid assay, this does not involve chimeric genes |
| D. | Affinity purification based on the epitope will then extract all the proteins attached to the bait protein from cell lysates |
| Answer» D. Affinity purification based on the epitope will then extract all the proteins attached to the bait protein from cell lysates | |
| 56. |
Which of the following is incorrect about Yeast-two-hybrid screens? |
| A. | The yeast-two-hybrid system uses the transcription of a reporter gene driven by the Gal4 transcription factor to monitor whether or not two proteins are interacting |
| B. | The DNA-binding domain chimeric protein will not bind upstream of the reporter gene |
| C. | If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed |
| D. | Disadvantages of the method are that only pairwise interactions are tested, and not interactions that can only take place when multiple proteins come together, as well as a high false positive rate |
| Answer» C. If the activation domain chimeric protein interacts with the DNA-binding domain chimeric protein, the reporter gene will be transcribed | |
| 57. |
For proteins in stable complexes the average sequence identity is 46%, while for proteins in transient interactions it is 41%. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 58. |
Membership in a stable complex also differs from transient interaction in terms of evolutionary constraints upon sequence divergence. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 59. |
Correlation of gene expression for pairs of transiently interacting proteins is ______ compared to randomly chosen pairs of proteins. |
| A. | not significant |
| B. | only marginally significant |
| C. | totally significant |
| D. | significant to much extent |
| Answer» C. totally significant | |
| 60. |
Sets of proteins that are part of stable complexes and sets of proteins involved in transient interactions ____ in terms of the similarity in gene expression among the set of proteins. |
| A. | are similar |
| B. | differ |
| C. | are same |
| D. | show similar function |
| Answer» C. are same | |
| 61. |
Stable complexes consist of proteins that are _____ associated with each other, like many ____ proteins for instance. |
| A. | temporarily, oligomeric |
| B. | temporarily, monomeric |
| C. | permanently, oligomeric |
| D. | permanently, monomeric |
| Answer» D. permanently, monomeric | |
| 62. |
There exist three types of interactions between domains. Which of the following is not one of them? |
| A. | Stable complex |
| B. | Transient interaction |
| C. | Multi-domain protein |
| D. | Unstable interaction |
| Answer» E. | |
| 63. |
The physical contacts between domains are crucial for the functioning of the cellular machinery. |
| A. | True |
| B. | False |
| Answer» B. False | |