MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
In the case of a polyvalent display, the proteins can be cloned directly into a viral vector as a fusion to a capsid protein. |
| A. | False |
| B. | True |
| Answer» C. | |
| 2. |
In the case of polyvalent-display, all gp III are conjugated with peptide and this results in occasional loss of infectivity. |
| A. | False |
| B. | True |
| Answer» C. | |
| 3. |
Which of the following is used to create a large library of mutants for a given protein gene? |
| A. | Protein assay |
| B. | Phage display |
| C. | X-ray crystallography |
| D. | Error-prone PCR |
| Answer» E. | |
| 4. |
In the case of a monovalent display, the protein fusion can be constructed in a phagemid vector that carries a copy of the viral capsid gene. |
| A. | False |
| B. | True |
| Answer» C. | |
| 5. |
Which of the following proteins are involved in initiating phage assembly and maintaining the stability of the viral particle? |
| A. | gp III and gp VIII |
| B. | gp VIII and gp II |
| C. | gp VI and gp IV |
| D. | gp VII and gp IX |
| Answer» E. | |
| 6. |
Which of the following is not true for error-prone PCR? |
| A. | It uses Taq polymerase which lacks proofreading ability |
| B. | Occurs under imperfect PCR conditions |
| C. | Involves annealing incompatible base-pairs to each other |
| D. | It is not a mutagenesis method |
| Answer» E. | |
| 7. |
Which of the following is the major coat protein of M13? |
| A. | gp VI |
| B. | gp V |
| C. | gp IV |
| D. | gp VIII |
| Answer» E. | |
| 8. |
M13 is a filamentous phage and it contains a circular single-stranded DNA genome. |
| A. | False |
| B. | True |
| Answer» C. | |
| 9. |
Which of the following bacteriophage is used in phage-display technology? |
| A. | T4 |
| B. | |
| C. | T2 |
| D. | M13 |
| Answer» E. | |
| 10. |
In the case of monovalent display, the valency of display depends on the ratio of phage containing phagemid vector and helper phage at the time of infection. |
| A. | False |
| B. | True |
| Answer» C. | |
| 11. |
Who pioneered phage-display technology? |
| A. | Kary Mullis |
| B. | Charles Chamberland |
| C. | Robert brown |
| D. | George Smith |
| Answer» E. | |
| 12. |
Which of the following is not a method of directed evolution? |
| A. | Phage display |
| B. | DNA shuffling |
| C. | Error-prone PCR |
| D. | Natural selection |
| Answer» E. | |
| 13. |
Which step is performed after the mutation of genes in directed evolution? |
| A. | X-ray crystallography |
| B. | Protein assay |
| C. | Protein screening |
| D. | Expression of mutant genes |
| Answer» E. | |
| 14. |
The starting point of both rational and irrational protein design is a wild-type protein. |
| A. | False |
| B. | True |
| Answer» C. | |
| 15. |
Only one cycle of directed evolution cycle is sufficient to obtain the desired protein molecule. |
| A. | True |
| B. | False |
| Answer» C. | |