MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Dwell volume is defined as |
| A. | the volume of solvent contained in a liquid chromatographic column |
| B. | the time required for the gradient to reach the column |
| C. | the volume of the column between the point at which solvents are mixed and the beginning of the column |
| D. | none of these |
| Answer» D. none of these | |
| 2. |
Void volume refers to the |
| A. | total volume of eluent in the column the remainder being taken up by the packing material |
| B. | the volume of solvent contained in a liquid chromatographic column |
| C. | the time required for the gradient to reach the column |
| D. | the volume of the column between the point at which solvents are mixed and the beginning of the column |
| Answer» B. the volume of solvent contained in a liquid chromatographic column | |
| 3. |
In normal phase HPLC, there is a |
| A. | non polar solvent/polar column |
| B. | polar solvent/non-polar column |
| C. | non polar solvent/non-polar column |
| D. | any of the above |
| Answer» B. polar solvent/non-polar column | |
| 4. |
An isocratic elution in HPLC is one in which the composition of the solvent |
| A. | remains constant |
| B. | changes continuously |
| C. | changes in a series of steps |
| D. | none of these |
| Answer» B. changes continuously | |
| 5. |
In reversed phase HPLC, there is a |
| A. | non polar solvent/polar column |
| B. | polar solvent/non-polar column |
| C. | non polar solvent/non-polar column |
| D. | any of the above |
| Answer» C. non polar solvent/non-polar column | |
| 6. |
Which of the following statements is true for a refractive index detector in HPLC? |
| A. | It is more sensitive than a UV detector |
| B. | It can only be used for isocratic elutions |
| C. | It does not respond to many solutes |
| D. | none of above |
| Answer» D. none of above | |
| 7. |
A gradient elution in HPLC is one in which the composition of the solvent |
| A. | remains constant |
| B. | is changed continuously or in a series of steps |
| C. | both (a) and (b) |
| D. | none of the above |
| Answer» C. both (a) and (b) | |
| 8. |
An eluotropic series |
| A. | ranks solvents by their relative abilities to displace solutes from a given absorbent |
| B. | ranks column packing material by their relative abilities to retain solutes on the column |
| C. | is a measure of the solvent adsorption energy |
| D. | none of the above |
| Answer» B. ranks column packing material by their relative abilities to retain solutes on the column | |
| 9. |
HPLC stands for |
| A. | High Pressure Liquid Chromatography |
| B. | High Performance Liquid Chromatography |
| C. | both (a) and (b) |
| D. | Highly Placed Liquid Chromatography |
| Answer» D. Highly Placed Liquid Chromatography | |
| 10. |
The eluent strength is a measure of |
| A. | solvent adsorption energy |
| B. | solvent absorption energy |
| C. | solvent diffusivity |
| D. | solvent mixing index |
| Answer» B. solvent absorption energy | |
| 11. |
For a typical adsorbent such as silica gel, the most popular pore diameters are |
| A. | 10 and 50 A |
| B. | 60 and 100 A |
| C. | 100 and 150 A |
| D. | 150 and 200 A |
| Answer» C. 100 and 150 A | |
| 12. |
HPLC methods include |
| A. | liquid/liquid (partition) chromatography |
| B. | liquid/solid (adsorption) chromatography |
| C. | ion exchange and size exclusion chromatography |
| D. | all of the above |
| Answer» E. | |
| 13. |
Which can be used as a mobile phase in HPLC applications? |
| A. | Any compound with solubility in liquid |
| B. | Any compound with limited solubility in liquid |
| C. | Any compound with non-solubility in liquid |
| D. | Any of the above |
| Answer» B. Any compound with limited solubility in liquid | |
| 14. |
Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)? |
| A. | Greater sensitivity and reusable columns |
| B. | Ideal for ionic species and large molecules |
| C. | Sample recovery |
| D. | All of the above |
| Answer» E. | |
| 15. |
Column efficiency is measured in terms of number of plates which is |
| A. | inversely related to the square of the peak width |
| B. | directly related to the square of the peak width |
| C. | inversely related to the cube root of the peak width |
| D. | directly related to the square of the peak width |
| Answer» B. directly related to the square of the peak width | |