MCQOPTIONS
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MCQOPTIONS
This section includes 9 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by |
| A. | SDS-PAGE electrophoresis |
| B. | gel filtration chromatography |
| C. | combining information from (a)and (b) |
| D. | isoelectric focusing |
| Answer» D. isoelectric focusing | |
| 2. |
Proteins are separated in an SDS-PAGE experiment on the basis of their |
| A. | positively charged side chains |
| B. | molecular weight |
| C. | negatively charged side chains |
| D. | different isoelectric points |
| Answer» C. negatively charged side chains | |
| 3. |
In a gel filtration column |
| A. | smaller proteins enter the beads more readily |
| B. | large proteins elute first |
| C. | both (a) and (b) |
| D. | large proteins enter the beads more readily |
| Answer» D. large proteins enter the beads more readily | |
| 4. |
In SDS-PAGE, the protein sample is first |
| A. | treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis |
| B. | fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. |
| C. | treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis |
| D. | none of the above |
| Answer» B. fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. | |
| 5. |
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in |
| A. | both proteins migrate to the anode |
| B. | histones migrate to the anode and myoglobin migrates to the cathode |
| C. | histones migrate to the cathode and myoglobin migrates to the anode |
| D. | both proteins migrate to the cathode |
| Answer» D. both proteins migrate to the cathode | |
| 6. |
In isoelectric focusing, proteins are separated on the basis of their |
| A. | relative content of positively charged residue only |
| B. | relative content of negatively charged residue only |
| C. | size |
| D. | relative content of positively and negatively charged residue |
| Answer» E. | |
| 7. |
In an SDS-PAGE |
| A. | proteins are denatured by the SDS |
| B. | proteins have the same charge-to-mass ratio |
| C. | smaller proteins migrate more rapidly through the gel |
| D. | all of the above |
| Answer» E. | |
| 8. |
In a native PAGE, proteins are separated on the basis of |
| A. | net negative charge |
| B. | net charge and size |
| C. | net positive charges size |
| D. | net positive charge |
| Answer» C. net positive charges size | |
| 9. |
Proteins can be visualized directly in gels by |
| A. | staining them with the dye |
| B. | using electron microscope only |
| C. | measuring their molecular weight |
| D. | none of these |
| Answer» B. using electron microscope only | |