MCQOPTIONS
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MCQOPTIONS
This section includes 22 Mcqs, each offering curated multiple-choice questions to sharpen your Separation Processes knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Proteins can be visualized directly in gels by |
| A. | staining them with the dye |
| B. | using electron microscope only |
| C. | measuring their molecular weight |
| D. | none of these |
| Answer» B. using electron microscope only | |
| 2. |
In isoelectric focusing, proteins are separated on the basis of their |
| A. | relative content of positively charged residue only |
| B. | relative content of negatively charged residue only |
| C. | size |
| D. | relative content of positively and negatively charged residue |
| Answer» E. | |
| 3. |
In SDS-PAGE, the protein sample is first |
| A. | treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis |
| B. | fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. |
| C. | treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis |
| D. | none of the above |
| Answer» B. fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. | |
| 4. |
In an SDS-PAGE |
| A. | proteins are denatured by the SDS |
| B. | proteins have the same charge-to-mass ratio |
| C. | smaller proteins migrate more rapidly through the gel |
| D. | all of the above |
| Answer» E. | |
| 5. |
In a native PAGE, proteins are separated on the basis of |
| A. | net negative charge |
| B. | net charge and size |
| C. | net positive charges size |
| D. | net positive charge |
| Answer» C. net positive charges size | |
| 6. |
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by |
| A. | SDS-PAGE electrophoresis |
| B. | gel filtration chromatography |
| C. | combining information from (a)and (b) |
| D. | isoelectric focusing |
| Answer» D. isoelectric focusing | |
| 7. |
In a gel filtration column |
| A. | smaller proteins enter the beads more readily |
| B. | large proteins elute first |
| C. | both (a) and (b) |
| D. | large proteins enter the beads more readily |
| Answer» D. large proteins enter the beads more readily | |
| 8. |
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in |
| A. | both proteins migrate to the anode |
| B. | histones migrate to the anode and myoglobin migrates to the cathode |
| C. | histones migrate to the cathode and myoglobin migrates to the anode |
| D. | both proteins migrate to the cathode |
| Answer» D. both proteins migrate to the cathode | |
| 9. |
Proteins are separated in an SDS-PAGE experiment on the basis of their |
| A. | positively charged side chains |
| B. | molecular weight |
| C. | negatively charged side chains |
| D. | different isoelectric points |
| Answer» C. negatively charged side chains | |
| 10. |
What cannot be a reason for using electrophoresis? |
| A. | Comparing two sets of DNA |
| B. | Organizing DNA by shape of backbone |
| C. | Organizing DNA fragments from largest to smallest |
| D. | Organizing DNA in order we can see |
| Answer» C. Organizing DNA fragments from largest to smallest | |
| 11. |
When is electrophoresis not used? |
| A. | Separation of proteins |
| B. | Separation of amino acids |
| C. | Separation of Lipids |
| D. | Separation of nucleic acids |
| Answer» D. Separation of nucleic acids | |
| 12. |
The electrophoretic mobility denoted as µ is mathematically expressed as: |
| A. | VE |
| B. | E/V |
| C. | 1/EV |
| D. | V/E |
| Answer» E. | |
| 13. |
WHAT_CANNOT_BE_A_REASON_FOR_USING_ELECTROPHORESIS??$ |
| A. | Comparing two sets of DNA |
| B. | Organizing DNA by shape of backbone |
| C. | Organizing DNA fragments from largest to smallest |
| D. | Organizing DNA in order we can see |
| Answer» C. Organizing DNA fragments from largest to smallest | |
| 14. |
WHEN_IS_ELECTROPHORESIS_NOT_USED??$ |
| A. | Separation of proteins |
| B. | Separation of amino acids |
| C. | Separation of Lipids |
| D. | Separation of nucleic acids |
| Answer» D. Separation of nucleic acids | |
| 15. |
Which of the following factors does not influence electrophoretic mobility? |
| A. | Molecular weight |
| B. | Shape of molecule |
| C. | Size of molecule |
| D. | Stereochemistry of molecule |
| Answer» E. | |
| 16. |
The electrophoretic mobility denoted as µ is mathematically expressed as:$ |
| A. | VE |
| B. | E/V |
| C. | 1/EV |
| D. | V/E |
| Answer» E. | |
| 17. |
If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is: |
| A. | SDS PAGE |
| B. | Affinity Electrophoresis |
| C. | Electro focusing |
| D. | Free flow electrophoresis |
| Answer» B. Affinity Electrophoresis | |
| 18. |
What does the electrophoresis apparatus consist of? |
| A. | Gel, buffer chamber and fire pack |
| B. | Buffer chamber and electrophoresis unit |
| C. | Electrophoresis unit and gel separator |
| D. | Power pack and electrophoresis unit |
| Answer» E. | |
| 19. |
Which of the following statements is true about migration of biomolecules? |
| A. | The rate of migration is directly proportional to the resistance of medium |
| B. | Rate of migration is directly proportional to current |
| C. | Low voltage is used for separation of high mass molecules |
| D. | Rate of migration is inversely proportional to current |
| Answer» C. Low voltage is used for separation of high mass molecules | |
| 20. |
The speed of migration of ions in electric field depends upon: |
| A. | Shape and size of molecule |
| B. | Magnitude of charge and shape of molecule |
| C. | Magnitude of charge shape and mass of molecule |
| D. | Magnitude of charge and mass of molecule |
| Answer» C. Magnitude of charge shape and mass of molecule | |
| 21. |
Electrophoresis was developed by: |
| A. | Tswett |
| B. | Tsvedberg |
| C. | Tiselius |
| D. | Sanger |
| Answer» D. Sanger | |
| 22. |
Which technique separates charged particles using electric field? |
| A. | Hydrolysis |
| B. | Electrophoresis |
| C. | Protein synthesis |
| D. | Protein denaturing |
| Answer» C. Protein synthesis | |