MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Repressor molecules bind to __________ |
| A. | Operator |
| B. | Promoter |
| C. | Enhancer |
| D. | Hormone response element |
| Answer» B. Promoter | |
| 2. |
Which of the following enzyme is required for end to end joining of DNA? |
| A. | DNA ligase |
| B. | Restriction endonuclease |
| C. | RNA polymerase |
| D. | DNA polymerase |
| Answer» B. Restriction endonuclease | |
| 3. |
What is a DNA library? |
| A. | A DNA fragment inserted into a vector |
| B. | A general collection of all genes sequenced thus far |
| C. | All DNA fragments identified with a probe |
| D. | A collection of DNA fragments that make up the entire genome of a particular organism |
| Answer» E. | |
| 4. |
In which stage of genetic engineering a probe is used? |
| A. | Cleaving DNA |
| B. | Recombining DNA |
| C. | Cloning |
| D. | Screening |
| Answer» E. | |
| 5. |
E.cor1 is a __________ |
| A. | DNA ligase enzyme |
| B. | Restriction endonuclease |
| C. | A vector used for insulin synthesis |
| D. | A plasmid used as a vector |
| Answer» C. A vector used for insulin synthesis | |
| 6. |
The extra chromosomal, self-replicating, closed, double stranded and circular DNA molecule is generally termed as __________ |
| A. | Chromosome |
| B. | Plasmid |
| C. | Genomic DNA |
| D. | Bacteriophage |
| Answer» C. Genomic DNA | |
| 7. |
To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added? |
| A. | MgCl2 |
| B. | CaCl2 |
| C. | NaCl |
| D. | HCl |
| Answer» C. NaCl | |
| 8. |
Generally a plasmid vector contains how many elements? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 9. |
Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector? |
| A. | Alkaline phosphatase and terminal transferase |
| B. | Exonuclease III and alkaline phosphatase |
| C. | Bacteriophage lambda exonuclease and terminal transferase |
| D. | Exonuclease III and terminal transferase |
| Answer» D. Exonuclease III and terminal transferase | |
| 10. |
Which of the following is true about restriction endonucleases? |
| A. | Type I and II requires ATP to move along DNA |
| B. | Type I, II and III requires ATP to move along DNA |
| C. | Type II requires no ATP and cleaves DNA within recognition sequence |
| D. | Type II requires ATP and cleaves DNA within recognition sequence |
| Answer» D. Type II requires ATP and cleaves DNA within recognition sequence | |
| 11. |
Which of the following can be termed as a restriction modification system? |
| A. | Restriction endonuclease + methylase |
| B. | DNA ligase + methylase |
| C. | Restriction endonuclease + acetylase |
| D. | DNA ligase + acetylase |
| Answer» B. DNA ligase + methylase | |
| 12. |
Which of the following enzyme is responsible for making a DNA copy from RNA? |
| A. | Reverse transcriptase |
| B. | DNA polymerase |
| C. | RNA polI |
| D. | RNA polII |
| Answer» B. DNA polymerase | |
| 13. |
Which of the following does not act as a restriction enzyme? |
| A. | EcorI |
| B. | BamHI |
| C. | HindIII |
| D. | polydeoxyribonucleotide synthase |
| Answer» E. | |