MCQOPTIONS
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MCQOPTIONS
This section includes 137 Mcqs, each offering curated multiple-choice questions to sharpen your Life Sciences knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is NOT the major function of the serum? |
| A. | Promotion of tuber and bulb formation |
| B. | Stimulate cell growth |
| C. | Enhance cell attachment |
| D. | Provide transport proteins |
| Answer» B. Stimulate cell growth | |
| 2. |
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are |
| A. | transcriptional regulators and are essential for viral replication |
| B. | not necessary for viral replication |
| C. | coding for virus coat protein |
| D. | important for packaging of viral DNA into viral head |
| Answer» B. not necessary for viral replication | |
| 3. |
Range of optimum glucose concentration present in the culture media is |
| A. | 5.5 - 55 mmol/litre |
| B. | 55 - 75 mmol/litre |
| C. | 75-105 mmol/litre |
| D. | 105-150 mmol/litre |
| Answer» B. 55 - 75 mmol/litre | |
| 4. |
Which of the following is/are the method of transfection for making transgenic animals? |
| A. | Transfer of whole nuclei |
| B. | Transfer of whole individual chromosomes or fragment |
| C. | Transfer of DNA |
| D. | All of the above |
| Answer» E. | |
| 5. |
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via |
| A. | oxidative pathway |
| B. | anaerobic pathway |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» B. anaerobic pathway | |
| 6. |
Which of the following is the word used to describe what happens when the nucleus of a sperm joins with the nucleus of an egg cell? |
| A. | Sex |
| B. | Fertilisation |
| C. | Intercourse |
| D. | Reproduction |
| Answer» C. Intercourse | |
| 7. |
Cloning is a method by which numbers of genetically identical organisms are derived from a single organism by |
| A. | vegetative propagation |
| B. | vegetative initiation |
| C. | vegetative termination |
| D. | none of these |
| Answer» B. vegetative initiation | |
| 8. |
Retroviruses have advantage for being used as vector for animal cells because |
| A. | they cover a wide host range |
| B. | infection does not lead to cell death, infected cells produce virus over an indefinite period |
| C. | viral gene expression is driven by strong promoters |
| D. | all of the above |
| Answer» E. | |
| 9. |
Fusion of karyoplast with the enucleated cell is achieved in presence of |
| A. | cytochalasin B |
| B. | polyethylene glycol |
| C. | both (a) and (b) |
| D. | alcohol |
| Answer» C. both (a) and (b) | |
| 10. |
Cells which have undergone transformation frequently become |
| A. | anchorage independent |
| B. | anchorage dependent |
| C. | stable |
| D. | unstable |
| Answer» B. anchorage dependent | |
| 11. |
Embryo transfer (ET) refers to the technique by which fertilized ova are collected from the reproductive tract of a genetically |
| A. | superior female and transferred to the inferior |
| B. | inferior female and transferred to the superior |
| C. | balanced female |
| D. | none of the above |
| Answer» B. inferior female and transferred to the superior | |
| 12. |
Chromosomes may be isolated from metaphase cells by |
| A. | hypertonic lysis |
| B. | hypotonic lysis |
| C. | either (a) or (b) |
| D. | isotonic lysis |
| Answer» C. either (a) or (b) | |
| 13. |
The technique of organ culture may be divided on the basis of employing |
| A. | solid medium |
| B. | liquid medium |
| C. | both (a) and (b) |
| D. | semi-solid medium |
| Answer» D. semi-solid medium | |
| 14. |
The human fibroplast is a classical example of |
| A. | stable primary cell lines |
| B. | established cell lines |
| C. | cell transformations |
| D. | none of these |
| Answer» B. established cell lines | |
| 15. |
Superovulation is primarily the result of |
| A. | decreased atresia in medium and large follicles (>1.7 mm) |
| B. | increased atresia in medium and large follicles (>1.7 mm) |
| C. | increased atresia in the large follicles (<1.7 mm) |
| D. | decreased atresia in small follicles (<1.7 mm) |
| Answer» B. increased atresia in medium and large follicles (>1.7 mm) | |
| 16. |
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) |
| A. | 0.05-5 nmol of O2/cell/h |
| B. | 5 - 10 nmol of O2 /cell/h |
| C. | 0.05-5 pmol of O2/cell/h |
| D. | 5 - 10 pmol of O2/cell/h |
| Answer» D. 5 - 10 pmol of O2/cell/h | |
| 17. |
In which year, Dolly the first mammalian clone was born? |
| A. | 1995 |
| B. | 1997 |
| C. | 1996 |
| D. | 1998 |
| Answer» D. 1998 | |
| 18. |
Range of optimum glutamine concentration present in the culture media is |
| A. | 1-2 mmol/litre |
| B. | 2-7 mmol/litre |
| C. | 7-15 mmol/litre |
| D. | 15 - 20 mmol/litre |
| Answer» C. 7-15 mmol/litre | |
| 19. |
In transgenic fish, the genes are introduced by |
| A. | microinjection in fish |
| B. | viruses |
| C. | transfer of whole nuclei |
| D. | all of these |
| Answer» B. viruses | |
| 20. |
When the cells are enucleated and centrifuged, nuclei |
| A. | detach from cell and settle at the bottom |
| B. | detach from cell and remain in the supernatant |
| C. | nuclei remain attached to the cell |
| D. | none of the above |
| Answer» B. detach from cell and remain in the supernatant | |
| 21. |
Which of the following viruses are used as vectors for transferring genes to animal cells? |
| A. | SV 40 virus |
| B. | Bovine papilloma virus (BVP) |
| C. | Retroviruses |
| D. | All of these |
| Answer» E. | |
| 22. |
The number of follicles at any particular stage is governed by |
| A. | the rate of entry of dormant follicles |
| B. | the rate of growth of follicles |
| C. | the rate of loss of follicles |
| D. | all of the above |
| Answer» E. | |
| 23. |
Animal pharming can be defined as |
| A. | growing animals for farming |
| B. | programming animals to produce novel products |
| C. | generating transgenic animals for farming |
| D. | none of the above |
| Answer» C. generating transgenic animals for farming | |
| 24. |
Which of the following best describes artificial insemination? |
| A. | Transplanting an embryo into the uterus |
| B. | Fertilization of an egg in a test tube |
| C. | Selectively breeding healthy animals |
| D. | Taking the sperm and placing it directly |
| Answer» E. | |
| 25. |
Some types of variation are due to changes in the genetic material. What is this type of change called? |
| A. | Fertilisation |
| B. | Mutation |
| C. | Radiation |
| D. | Sterilisation |
| Answer» C. Radiation | |
| 26. |
Toxicity due to accumulation of ammonia can be overcome |
| A. | by substituting glutamine by glutamate |
| B. | by controlled addition of glutamine at low level |
| C. | by removal of ammonia or ammonium from culture medium |
| D. | all of the above |
| Answer» E. | |
| 27. |
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called |
| A. | established cell lines |
| B. | primary cell lines |
| C. | secondary cell lines |
| D. | propagated cell lines |
| Answer» B. primary cell lines | |
| 28. |
What are the main constituents of culture for animal cell growth? |
| A. | Glucose and Glutamine |
| B. | Growth factors |
| C. | Cytokines |
| D. | All of these |
| Answer» B. Growth factors | |
| 29. |
Which protein has been produced generating a transgenic sheep that is used for replacement therapy for individuals at risk from emphysema? |
| A. | Plasminogen activator (tPA) |
| B. | α-anti trypsin (AAT) |
| C. | Casein |
| D. | Amyloid precursor proteins |
| Answer» C. Casein | |
| 30. |
For the transfer of whole individual chromosomes, they are isolated from the cells at |
| A. | prophase |
| B. | metaphase |
| C. | telophase |
| D. | anaphase |
| Answer» C. telophase | |
| 31. |
Which of the following is not the explantation technique? |
| A. | Slide culture |
| B. | Carrel flask culture |
| C. | Roller test tube culture |
| D. | Adherent primary culture |
| Answer» E. | |
| 32. |
Transgenic goats produce a variant of human tissue type plasminogen activator protein in |
| A. | blood |
| B. | urine |
| C. | milk |
| D. | muscles |
| Answer» D. muscles | |
| 33. |
For the rapid genetic progress, embryo transfer (ET) |
| A. | will be as potent a tool as AI (artificial insemination) |
| B. | will not be as potent a tool as AI (artificial insemination) |
| C. | ET can't be used at all |
| D. | will be a superior tool than AI |
| Answer» C. ET can't be used at all | |
| 34. |
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of |
| A. | 6 amino acids |
| B. | 8 amino acids |
| C. | 10 amino acids |
| D. | 12 amino acids |
| Answer» E. | |
| 35. |
Which of the following are produced in high copy number? |
| A. | YEp |
| B. | YRp |
| C. | Both (a) and (b) |
| D. | YCp |
| Answer» D. YCp | |
| 36. |
Maximum amount of foreign DNA introduced into SV 40 viruses is |
| A. | 10 kb |
| B. | 5 kb |
| C. | 2.5 kb |
| D. | 1 kb |
| Answer» D. 1 kb | |
| 37. |
Which of the following is/are commonly used promoter of S. cerevisiae? |
| A. | Galactokinase (GAL 1) |
| B. | Alcohol dehydrogenase II (ADH 2) |
| C. | Phosphoglycerate kinase (PGK) |
| D. | All of the above |
| Answer» E. | |
| 38. |
Production of transgenic animals require transfections of |
| A. | eggs or embryos |
| B. | stem cells |
| C. | red blood cells |
| D. | all of these |
| Answer» B. stem cells | |
| 39. |
How does CO2 help in the cell metabolism during cell culture? |
| A. | It participates in the de novo synthesis of purines and pyrimidines |
| B. | Helps in the cells respiration |
| C. | For monitoring pH of the culture |
| D. | All of the above |
| Answer» B. Helps in the cells respiration | |
| 40. |
Enucleation of the cells can be achieved by treating the cells with |
| A. | polyethylene glycol |
| B. | cytochalasin B |
| C. | both (a) and (b) |
| D. | alcohol |
| Answer» C. both (a) and (b) | |
| 41. |
Which of these carries the thousands of genes needed to allow animal and plant cells to pass on inherited characteristics? |
| A. | Chromosomes |
| B. | Chromatogram |
| C. | Chromatography |
| D. | Chromium |
| Answer» B. Chromatogram | |
| 42. |
For culturing, plasma from the adult chicken is preferred to mammalian plasma because |
| A. | it forms a clear and solid coagulum even after dilution |
| B. | it is too opaque |
| C. | it doesn't produce solid clots |
| D. | it forms a semi solid coagulum |
| Answer» B. it is too opaque | |
| 43. |
The major problem associated with the isolation of free cells and cell aggregates from organs is that of |
| A. | releasing the cells from their supporting matrix |
| B. | inhibiting the cells from their supporting matrix |
| C. | disintegrating the cells from their supporting matrix |
| D. | none of the above |
| Answer» B. inhibiting the cells from their supporting matrix | |
| 44. |
Karyoplast is |
| A. | cells devoid of cell wall |
| B. | nuclei |
| C. | nuclei with only some residual plasma membrane |
| D. | cell with nucleus |
| Answer» D. cell with nucleus | |
| 45. |
pH of culture medium is initially controlled by |
| A. | presence of CO2 |
| B. | presence of bicarbonate buffer |
| C. | addition of bases |
| D. | none of these |
| Answer» C. addition of bases | |
| 46. |
Transgenic goats have been used to produce which of the following protein that is used for dissolving blood clots? |
| A. | Amyloid precursor protein |
| B. | α1-anti trypsin (AAT) |
| C. | Casein |
| D. | A variant of human tissue-type plasminogen activator |
| Answer» E. | |
| 47. |
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping? |
| A. | Down's syndrome |
| B. | Turner's syndrome |
| C. | Klinefelter's syndrome |
| D. | all of these |
| Answer» E. | |
| 48. |
Which of these established cell lines originate from a mouse embryo? |
| A. | 3T3 |
| B. | BHK |
| C. | HeLa |
| D. | BTK |
| Answer» B. BHK | |
| 49. |
Excess CO2 suppress cell growth and productivity by |
| A. | inhibiting respiration |
| B. | altering intracellular pH by diffusing across cell membrane |
| C. | both (a) and (b) |
| D. | altering pH of the medium |
| Answer» D. altering pH of the medium | |
| 50. |
Which one of the following is the most stable vector? |
| A. | YEp |
| B. | YRp |
| C. | YAC |
| D. | YIp |
| Answer» D. YIp | |